T. Smith

Cryo-EM Structure of the R388 plasmid conjugative pilus reveals a helical polymer characterised by an unusual pilin/phospholipid binary complex

The paper presents the cryo-EM structure of the R388 plasmid conjugative pilus, revealing a unique pilin/phospholipid complex and structural differences from other conjugative pili.

Drivers of Bird Communities in an Urban Neighborhood Vary by Scale

This study examines how bird community metrics relate to vegetation and air pollution at multiple spatial scales in an urban neighborhood, finding positive associations with greenness and negative associations with air pollution.

Genome-wide CRISPR screen identifies KEAP1 perturbation as a vulnerability of ARID1A-deficient cells

The paper identifies KEAP1 as a genetic dependency in ARID1A-deficient clear cell ovarian carcinoma cells through a genome-wide CRISPR screen, showing KEAP1 perturbation leads to growth inhibition.

CZ CELLxGENE Discover: A single-cell data platform for scalable exploration, analysis and modeling of aggregated data

CZ CELLxGENE Discover provides a platform for scalable exploration and analysis of aggregated single-cell transcriptomic data across multiple studies.

AnophelesModel: An R package to interface mosquito bionomics, human exposure and intervention effects with models of malaria intervention impact

AnophelesModel integrates mosquito and human data to assess malaria intervention impacts across regions.

Fusion crystallization reveals the behavior of both the 1TEL crystallization chaperone and the TNK1 UBA domain

The paper describes the use of TELSAM fusion crystallization to determine the structure of the TNK1 UBA domain and its interaction with polyubiquitin.

Sex-specific Proximal Tubular Cell differentiation pathways identified by single-nucleus RNA

This study identifies sex-specific differentiation pathways in proximal tubular cells using single-nucleus RNA sequencing in mice.

PERK-mediated induction of miR-5p and miR-3p arms of miR-616 regulates cell growth by targeting c-MYC

The paper shows that PERK-mediated induction of miR-5p and miR-3p arms of miR-616 regulates cell growth by targeting c-MYC, with miR-616 expression linked to survival outcomes in breast cancer.

Chemical structures of cyclic ADP ribose (cADPR) isomers and the molecular basis of their production and signaling

The paper reports the chemical structures of two cADPR isomers, their production mechanism, and their roles in antiviral signaling and plant immunity suppression.

CD8 T Cell Memory Inflation is Driven by Latent CMV Infection of Lymphatic Endothelial Cells

The paper identifies lymphatic endothelial cells as the source of antigen presentation driving CD8 T cell memory inflation during latent CMV infection.

A system-wide quantitative map of RNA and protein subcellular localisation dynamics

The paper introduces LoRNA and a re-engineered LOPIT method to jointly map RNA and protein subcellular localisation, revealing dynamics during the unfolded protein response.

Preclinical Efficacy of IMM-BCP-01, a Highly Active Patient-Derived Anti-SARS-CoV-2 Antibody Cocktail

The paper identifies and characterizes an antibody cocktail, IMM-BCP-01, that neutralizes SARS-CoV-2 variants in vitro and inhibits infection in vivo through Spike Trimer dissociation and effector responses.

Mapping Molecular Gene Signatures Among Respiratory Viruses Based on Large-Scale and Genome-wide Transcriptomics Analysis

This study identifies gene signatures for five respiratory viruses using genome-wide transcriptomics, revealing shared and distinct gene regulation patterns and potential markers for infection and co-infection.

Global prediction of candidate R-loop binding and R-loop regulatory proteins

The paper develops a random forest classifier to predict R-loop binding proteins using proteomic features and amino acid composition, and validates new R-loop modulating proteins through CRISPR screening.

TELSAM polymers accelerate crystallization of fused target proteins by stabilizing minimal crystal contacts and in the absence of direct inter-TELSAM contacts

This study shows that TELSAM polymers enhance protein crystallization by stabilizing minimal crystal contacts and enabling rapid crystallization without direct inter-TELSAM contacts.

Development of GPC2-directed chimeric antigen receptors using mRNA for pediatric brain tumors

This study develops mRNA chimeric antigen receptor T cells targeting GPC2 on pediatric brain tumors, showing significant tumor regression and prolonged survival without toxicity.

Nucleic acid delivery of immune-focused SARS-CoV-2 nanoparticles drive rapid and potent immunogenicity capable of single-dose protection

This study develops glycan-modified SARS-CoV-2 nanoparticle vaccines that induce potent neutralizing antibodies and single-dose protection against SARS-CoV-2 in multiple animal models.

Reference transcriptomes of porcine peripheral immune cells created through bulk and single-cell RNA sequencing

The paper defines porcine peripheral blood immune cell transcriptomes using bulk and single-cell RNA sequencing, revealing similarities to human data and providing resources for immunogenetic studies.

Unbiased interrogation of memory B cells from convalescent COVID-19 patients reveals a broad antiviral humoral response targeting SARS-CoV-2 antigens beyond the spike protein

Multimodal Analysis for Human ex vivo Studies Shows Extensive Molecular Changes from Delays in Blood Processing

This study shows that delayed blood processing causes significant changes in single-cell transcriptomes and plasma proteomes within 6 hours, potentially confounding disease-related biological variance.

Structural basis for divergent and convergent evolution of catalytic machineries in plant aromatic amino acid decarboxylase proteins

This paper presents crystal structures of four plant AAAD paralogs, revealing structural features and mutations that underlie divergent substrate selectivity and convergent aldehyde synthase activity evolution.

Establishment of a pig influenza challenge model for evaluation of monoclonal antibody delivery platforms

This study establishes a pig influenza model to evaluate monoclonal antibody efficacy against H1N1, showing dose-dependent reduction in viral load and lung pathology.

Tests of hybridisation in Tetragonula stingless bees using multiple genetic markers.

The paper re-examines hybridisation in three Tetragonula stingless bee species using multiple genetic markers and finds no evidence of ongoing gene flow.

ASCL1 regulates neurodevelopmental transcription factors and cell cycle genes in glioblastoma

This study shows ASCL1 regulates key genes in glioblastoma, affecting cell proliferation and survival in a mouse model.

In-Depth Characterization and Validation in BRG1-Mutant Lung Cancers Define Novel Catalytic Inhibitors of SWI/SNF Chromatin Remodeling

This study validates novel BRM/BRG1 ATPase inhibitors as chemical probes for SWI/SNF chromatin remodeling and shows their therapeutic potential in BRG1-mut lung cancers.

Glycosylation of gigaxonin regulates intermediate filaments: Novel molecular insights into giant axonal neuropathy

This study identifies O-GlcNAcylation of gigaxonin as a regulatory mechanism linking metabolism to intermediate filament proteostasis, offering insights into giant axonal neuropathy.

Tuning the course of evolution on the biophysical fitness landscape of an RNA virus

This study predicts the evolutionary pathway of norovirus escaping antibodies using a biophysical fitness model, validated by experimental evolution in bulk culture and microfluidics.