T. Isogai

Spatial proteomics of ER tubules reveals CLMN, an ER-actin tether at focal adhesions that promotes cell migration

The paper maps the human ER subdomain proteome, identifies CLMN as an ER-actin tether at focal adhesions, and shows it promotes cell migration by regulating actin dynamics and calcium signaling.

RhoA activation promotes glucose uptake to elevate proliferation in MAPK inhibitor resistant melanoma cells.

This study identifies RhoA activation as a driver of glucose uptake and proliferation in MAPK inhibitor-resistant melanoma cells through ROCK-mediated IRS1 and PI3K pathway activation.

Keratin isoform shifts modulate motility signals during wound healing

The paper shows that keratin isoform shifts during wound healing modulate motility signals by activating myosin motors through isoform-specific interactions with vimentin shuttling kinases.

Granger-causal inference of the lamellipodial actin regulator hierarchy by live cell imaging without perturbation

This study uses Granger-causal inference on live cell imaging data to reconstruct cause-effect relationships among actin regulators in lamellipodia without perturbation, identifying distinct activation zones and roles.

u-track 3D: measuring and interrogating dense particle dynamics in three dimensions.

u-track 3D provides a software package for analyzing dense 3D particle dynamics in microscopy data by improving visualization, trajectory validation, and interactive exploration.

Direct Arp2/3-vinculin binding is essential for cell spreading, but only on compliant substrates and in 3D

The paper investigates the role of actin-binding proteins in cell spreading on 2D and 3D substrates, finding that Arp2/3-vinculin binding is critical for 3D spreading but not 2D.

A unified role for membrane-cortex detachment during cell protrusion initiation

The paper shows that membrane-cortex detachment is essential for initiating both pressure-based and actin-based protrusions, challenging the Brownian ratchet model and unifying protrusion regulation.

CLIC4 is regulated by RhoA-mDia2 signaling through Profilin-1 binding to modulate filopodia length

This study identifies RhoA-mDia2 signaling and Profilin-1 binding as regulators of CLIC4 translocation, which modulates filopodia length through actin dynamics.