E. Betzig

Oblique Line Scan Illumination Enables Expansive, Accurate and Sensitive Single Protein Measurements in Solution and in Living Cells

This paper introduces Oblique Line Scan (OLS) illumination to enhance single-molecule tracking in living cells and solution, enabling high-throughput and sensitive protein dynamics measurements.

Characterization, Comparison, and Optimization of Lattice Light Sheets

This paper compares and optimizes lattice light sheets to improve resolution and reduce photobleaching in light sheet microscopy.

u-track 3D: measuring and interrogating dense particle dynamics in three dimensions.

u-track 3D provides a software package for analyzing dense 3D particle dynamics in microscopy data by improving visualization, trajectory validation, and interactive exploration.

Adaptive fitness in APC-mutant cells requires chromosome rearrangements

The paper shows that APC-mutant cells gain adaptive fitness through chromosome rearrangements that restore AURKA activity and counteract mitotic defects.

Tiled Reconstruction Improves Structured Illumination Microscopy

This paper introduces a tiled reconstruction method to reduce artifacts in structured illumination microscopy (SIM) images by improving the reconstruction process.

Endocytic Clathrin Coats Develop Curvature at Early Stages of Their Formation

The paper uses live cell imaging to show clathrin-coated pits develop curvature early without requiring a flat-to-curved transition.

Correlative three-dimensional super-resolution and block face electron microscopy of whole vitreously frozen cells

This study develops a platform for 3D correlative cryogenic super-resolution and block-face EM of whole vitreously frozen cells, revealing new protein-ultrastructure relationships.

Augmin-mediated amplification of long-lived spindle microtubules directs plus-ends to kinetochores

The paper shows that Augmin amplifies long-lived microtubules, directing their plus-ends to kinetochores during spindle assembly in human cells.

Aurora B is required for programmed variations of cytokinesis during morphogenesis in the C. elegans embryo

The paper investigates cytokinesis variations during C. elegans embryonic morphogenesis and finds Aurora B kinase is essential for epithelial polarization and tissue formation.

Three-dimensional actin-based protrusions of migrating neutrophils are intrinsically lamellar and facilitate direction changes

This study uses lattice light sheet microscopy to analyze three-dimensional neutrophil pseudopods, revealing their lamellar structure and role in directional changes.