The Origins of ATP Dependence in Biological Nitrogen Fixation
D. Harris, H. Rucker, A. Garcia, Z.-Y. Yang, S. Chang, H. Feinsilber, B. Kacar, L. Seefeldt
This study experimentally reconstructs an ancestral nitrogenase enzyme and shows it strictly requires ATP hydrolysis for N2 reduction, decoupling cofactor selection from environmental availability.
bioRxiv · Feb 22, 2024 · PreprintEvolutionary Biology