Tuberculosis Research and Epidemiology

Rv0783c of Mycobacterium tuberculosis acts as a proton-motive force dependent multidrug efflux transporter involved in the efflux of structurally unrelated antibiotics and enhancing biofilm formation

This study identifies Rv0783c as a multidrug efflux transporter in Mycobacterium tuberculosis that enhances antibiotic resistance and biofilm formation.

Serum nutritional-metabolic recovery during tuberculosis treatment across BMI-defined undernutrition strata: A longitudinal proteomic reanalysis

This study reanalyzes longitudinal serum proteomics data from tuberculosis patients to assess how nutritional-metabolic recovery during treatment varies by baseline BMI, finding no significant BMI-dependent trajectories but an overall increase in a 36-protein summary axis.

ESX-5 Deletions in Mycobacterium tuberculosis Alter Macrophage Cytokine Signaling and Bacterial Heavy Metal Response

This study shows that ESX-5 deletions in Mtb reduce cytokine secretion in macrophages via a post-transcriptional mechanism and alter heavy metal response genes.

Enhancement of a STING Agonist Vaccine for Tuberculosis Using Locally Supercharged MS2 Viral Capsids

This paper develops a vaccine platform using MS2 viral capsids to deliver an Mtb antigen and STING agonist, reducing the required dose and cost while maintaining efficacy.

The Mycobacterium tuberculosis ESX effector promotes pyroptosis-dependent pathogenicity and dissemination

This study identifies EccB5 as a key Mtb effector that drives pyroptosis and enhances virulence by interacting with GSDMD and caspase-1.

Distinct transcriptional remodeling of immune and metabolic pathways in severe undernutrition during latent tuberculosis infection does not consistently predict tuberculosis progression across independent cohorts

The paper examines transcriptional changes in severe undernutrition during latent TB infection and finds inconsistent associations with TB progression across cohorts.

DDU384: A new preclinical candidate for the treatment of Tuberculosis

This paper identifies DDU384, a new compound with potent in vivo efficacy against tuberculosis in murine models, showing potential to improve current treatment options.

Early tuberculosis treatment remodels blood mRNA and small RNA, and a baseline host signature is associated with early culture conversion

This study identifies blood-based biomarkers that predict early tuberculosis culture conversion by analyzing mRNA and small RNA changes during treatment.

Two potent, functionally redundant MazF toxins inactivate the 16S rRNA anti-Shine-Dalgarno sequence and are differentially expressed in Mycobacterium tuberculosis

This study identifies two MazF toxins in Mycobacterium tuberculosis that target the 16S rRNA anti-Shine-Dalgarno sequence, showing differential expression under stress conditions.

Phylogenomic screening reveals idsA2 loss conferring nitroimidazole cross-resistance in Mycobacterium tuberculosis

A length- and mutation-load-corrected homoplasy screen of 1,813 MDR-TB genomes flags idsA2; its loss raises pretomanid MIC fourfold, delamanid and ethambutol twofold, via isoprenoid flux redirection.

Mycobacterium tuberculosis suppresses protective Th17 responses during infection

This study identifies factors by which Mycobacterium tuberculosis suppresses Th17 responses during infection, showing that ESX-1 and PDIM inhibit IL-23 production in dendritic cells.

Sphingosine-1-Phosphate Restrains Foamy Macrophage Formation and Modulates Inflammation in Multidrug-Resistant Tuberculosis

This study investigates the role of sphingosine-1-phosphate (S1P) in modulating foamy macrophage formation and inflammation in multidrug-resistant tuberculosis (MDR-TB).

Rv2061c, a Putative F420-Dependent Oxidoreductase, is Required for Tolerance to Malachite Green and Other Antimicrobials and Oxidative Stress Protection in Mycobacterium tuberculosis

This study identifies Rv2061c as a critical F420-dependent oxidoreductase that protects Mycobacterium tuberculosis from antimicrobials and oxidative stress.

High-throughput genetic interaction analysis in Mycobacterium tuberculosis identifies highly synergistic drug targets

This study uses CRISPRi screening in Mycobacterium tuberculosis to identify synergistic drug targets, finding that genetic interactions predict drug-drug synergies and identifying Pks13 as a key synergistic generalist.

Pulmonary Tuberculosis Is Associated with Body Site-Specific Alterations in the Respiratory and Gut Microbiome in Uganda

This study examines how pulmonary tuberculosis affects the respiratory and gut microbiomes in Ugandan adults, finding site-specific taxonomic differences but mixed results on community composition.

Excessive sample reagent incubation time increases the risk of false rifampin resistance results in Xpert(R) MTB/RIF Ultra tests performed on paucibacillary Mycobacterium tuberculosis sputum.

This study shows that extended incubation of paucibacillary sputum samples beyond 4 hours in sample reagent increases false rifampin resistance results in Xpert MTB/RIF Ultra tests.

A tissue time machine to forward-predict single-timepoint data applied to tuberculosis granulomas

The paper introduces a method to predict longitudinal outcomes from single-timepoint data using a virtual library of granulomas, applied to tuberculosis in non-human primates.

Discoidins are cytosolic lectins that recognize methylated L-rhamnose epitopes on mycobacterial glycolipids and glycopeptidolipids upon vacuole damage

The structural context of mutations in proteins predicts their effect on antibiotic resistance

The paper predicts antibiotic resistance mutations in Mycobacterium tuberculosis by integrating structural context with mutation data, finding that structural clustering improves prediction accuracy.

Development of inducible genetic systems to finely tune efficacy of InhA-targeting anti-tuberculous drugs

This paper develops CRISPRi-based inducible systems to fine-tune antibiotic susceptibility in Mycobacterium tuberculosis by targeting key genes involved in drug activation.

In-Depth Immune Profiling Reveals Cell-Type, Sex and Infection-Stage Dependent Differences for Tuberculosis Control

This study profiles the immune environment in male and female mice following TB infection and vaccination, identifying sex-specific immune markers and known protective responses.

ESX-1 remodels the Mycobacterium tuberculosis antigen storage compartment in macrophages

The paper investigates how Mtb remodels antigen storage compartments in macrophages using a split fluorescent protein reporter and subcellular spatial proteomics.

Scale- and genotype-dependent spatial concordance of Mycobacterium bovis in cattle and badgers

The paper examines spatial concordance of Mycobacterium bovis genotypes between cattle and badgers at different scales and finds it varies by genotype combination and spatial scale.

Mycobacterium tuberculosis inactivates host oxysterols to impair macrophage cholesterol homeostasis

This study identifies Mtb's enzymatic inactivation of host oxysterols as a mechanism to disrupt macrophage cholesterol homeostasis and generate treatment-responsive metabolic signatures in TB.

MTB-KB: A Curated Knowledgebase of Mycobacterium tuberculosis Related Studies

MTB-KB consolidates fragmented MTB research into a structured knowledgebase with a knowledge graph, enabling exploration of MTB-host interactions and treatment strategies.

Evolutionary dynamics of the insertion sequence IS6110 in the Mycobacterium tuberculosis complex

The paper analyzes the evolutionary dynamics of IS6110 in Mycobacterium tuberculosis complex strains using ancestral state reconstruction and identifies patterns of insertion and selection.

Mycobacterium tuberculosis requires riboflavin biosynthesis for survival in the host

This study shows that Mycobacterium tuberculosis needs riboflavin biosynthesis for survival during all stages of infection, suggesting it as a drug target.

Deep Learning-Based Spatial Immunoprofiling of Multiplex Immunofluorescence Images Distinguishes Tuberculosis Disease States in Diversity Outbred Mice

This study uses deep learning to analyze spatial immune patterns in tuberculosis-infected mice, identifying distinct granuloma features associated with asymptomatic, acute, and chronic disease states.

Polyketide synthase 12 is an in vivo essential source of novel mycolyl lipids in Mycobacterium tuberculosis

The paper identifies pks12 as an in vivo essential gene in Mtb by combining genomic, metabolomic, and phenotypic analyses, revealing its role in mycolyl lipid synthesis and host-pathogen interface structure.

Human memory CD4+ T cells recognize non-infected macrophage bystanders exposed to Mycobacterium tuberculosis-infected cells

The paper shows human memory CD4+ T cells recognize both Mtb-infected and bystander macrophages, with some TCRs specifically recognizing infected cells.