Microtubule and mitosis dynamics

The paper reports the structure of the γ-tubulin ring complex-capped microtubule, revealing how it nucleates and stabilizes 13-protofilament microtubules.

The paper derives expressions for critical concentrations of dynamically unstable polymers, showing how nucleotide hydrolysis and spontaneous nucleation rates affect their assembly.

The paper investigates the role of CetZ1 in Haloferax volcanii morphogenesis, showing that its GTP-dependent self-association and specific structural features are critical for cell shape development.

This study shows that KATANIN-mediated microtubule severing is essential for forming and functioning MTOCs in the liverwort Marchantia polymorpha.

This study investigates the role of PfCAP-H in condensin I complex assembly and karyokinesis during Plasmodium falciparum asexual proliferation, finding it essential for structural integrity and proper cell division.

The paper screens for genes affecting Drosophila female meiosis by testing RNAi constructs in germline-specific promoters to identify defects in chromosome congression and Mps1-GFP sequestration.

This study shows that the BUB-1 TPR domain directly binds phosphorylated KNL-1 motifs, essential for BUB-1-BUB-3 localization and function in C. elegans.

This study investigates whether low-intensity vibration affects microtubule dynamics in vitro and finds no significant changes compared to controls, unlike Taxol treatment.

This study shows Nek2A unclusters extra centrosomes and induces cell death in cancer cells, but not in normal cells, through interaction with KIF2C.

This study shows that gradual Cdk1 activation during G2 phase coordinates mitotic factor production and CDK1/PLK1 activation, and that WEE1 inhibition disrupts this coordination.

This study identifies distinct roles of Aurora A in regulating PAR protein localization during different stages of the C. elegans cell cycle, explaining varied polarity phenotypes observed in RNAi experiments.

This study shows that Kid (KIF22) forms a homodimer, moves processively along microtubules, and transports double-stranded DNA, reclassifying it as a processive and dimeric motor.

The paper investigates the relationship between microtubule length and spindle size in C. elegans meiosis, finding a positive correlation and demonstrating the role of microtubule length in determining spindle dimensions.

This study shows that F-actin, via Cofilin-1 and Arp2/3, promotes microtubule severing during cytokinetic abscission, independent of membrane scission.

This study edits endogenous tubulin to assess how different posttranslational modifications affect axonal growth and regeneration in C. elegans neurons.

This study shows that SAS-6 is essential for centriole formation in mouse embryos but not in mouse embryonic stem cells, highlighting context-dependent requirements.

The paper identifies two molecules that activate nitrotyrosine ligation to detyrosinated α-tubulin by targeting TTLL12, which suppresses this process, suggesting potential therapeutic applications for cancer and related pathologies.

This study identifies CKAP2 as a microtubule growth factor that regulates microtubule dynamics and ensures accurate chromosome segregation, with its loss leading to segregation errors and aneuploidy.

This study examines how human cell populations adapt to different levels of centriole amplification through long-term experimental evolution and fitness analysis.

This paper shows that STIL-mediated loss of BRCA1 functionality leads to DNA damage and centrosome amplification in cancer.

The paper shows that Ca2+ oscillations drive cytoplasmic backflow, which helps maintain spindle position during fertilized mouse egg division.

This study investigates HMMR's non-mitotic role in regulating TPX2 localization and microtubule dynamics in neurons, revealing its impact on neuronal morphology.

This study identifies a specialized Ran-GTP-driven spindle structure in medaka embryos that ensures accurate chromosome segregation during early cleavage divisions.

This paper studies how kinesin-1 motors transport liposome cargo through 2D and 3D microtubule intersections, finding that spatial relationships bias directional outcomes, with a higher straight-through rate at 3D intersections.

This study uses multiscale techniques to reveal the molecular structure of autoinhibited KIF5A, showing how its C-terminal tail contributes to autoinhibition and how mutations affect this state.

This paper shows that dysregulation of CycB:CDK disrupts cell cycle switches, leading to genome doubling, a common event in tumorigenesis.

This study investigates 3-epicaryoptin's anti-cancer effects on MCF-7 cells, showing it induces G2/M arrest and apoptosis by disrupting microtubules.

This study examines the development of tubulin-containing structures in Toxoplasma gondii using advanced imaging techniques, revealing distinct developmental patterns and symmetry changes.

The paper uses mass photometry to show heterogeneous distributions of kinesin-streptavidin complexes, attributing this to mixing ratios and biotinylation efficiency.

This study identifies CAPS as a microtubule-binding protein essential for spindle formation and mitosis, with potential implications for cancer biology.