Calcium signaling and nucleotide metabolism

Targeting Lysosomal MCOLN1/TRPML1 Ion Channels to Alleviate Diabetes Mellitus via a Ca2+- CaMKKβ-AMPK pathway

This study shows that activating TRPML1 ion channels improves glucose uptake and reduces hyperglycemia in a T2DM mouse model by enhancing AMPK phosphorylation through the Ca2+-CaMKKβ pathway.

Lysosomal signaling pathways influence heart rhythm, and regulate atrial function

This study investigates the role of lysosomal Ca2+ signaling in heart rhythm and atrial function, finding that NAADP pathway inhibition affects SAN pacemaker activity and cAMP production in atrial myocytes.

Three separate pancreatic acinar cell receptors employ different combinations of intracellular pathways to Ca2+ signalling

This study identifies distinct intracellular pathways used by three pancreatic acinar cell receptors to generate Ca2+ signals in response to low concentrations of secretagogues.

Alcohol-Evoked Calcium Signaling Drives Distinct Responses in Zebrafish Hepatocytes and Pancreatic Acinar Cells

This study shows alcohol exposure causes shared calcium signaling changes in zebrafish hepatocytes and pancreatic acinar cells, with tissue-specific molecular mechanisms involved.

Ryanodine receptor 1 is dispensable for CD4+ T-cell differentiation and effector function in intestinal inflammation models

This study shows that RYR1 in CD4+ T cells is not essential for their pathogenicity in intestinal inflammation models despite affecting calcium signaling.

TRPML1 loss drives lysosomal calcium failure and astrocyte dysfunction across Alzheimer's Disease progression

This study identifies TRPML1 loss as a cause of lysosomal calcium failure and astrocyte dysfunction in Alzheimer's Disease, showing that restoring TRPML1 can prevent disease progression.

ATG-9-Induced Lysosomal Membrane Permeabilization and Cell Death in a Caenorhabditis elegans model of Mucolipidosis type IV

This study identifies ATG-9-mediated lysosomal membrane permeabilization as a mechanism of cell death in a C. elegans model of Mucolipidosis type IV.

TRPML1 positions lysosomes and regulates actin-membrane linkers in astrocyte processes

This study investigates how TRPML1 regulates lysosome positioning and actin-membrane linkers in astrocyte processes, influencing synaptic contacts.

HIV-1 gp120-induced lysosomal stress responses are controlled by TRPML1 redox sensors

This study shows that TRPML1 redox activation mediates gp120-induced lysosomal stress and iron/redox imbalance, contributing to HAND pathogenesis.

The lysosomal cation channel TRPML1 regulates the oligodendrocyte cytoskeleton

This study shows TRPML1 regulates oligodendrocyte morphology and myelination through actin dynamics and calcium signaling during differentiation.

Protocol for assessing lysosomal ion channel function in mammalian cells using lysosomal patch-clamp technique

This paper provides a protocol for manual whole-endolysosome patch-clamp recordings from enlarged endolysosomes in mammalian cells.

JPT2/HN1L functions as an NAADP-binding protein in a cell type-specific manner

This study shows JPT2/HN1L is essential for NAADP-mediated Ca2+ release in T cells but not in cardiomyocytes, platelets, or mast cells.

CCZ1 is a modulator of TPC2 activity and melanoma cell migration

This study identifies CCZ1 as a modulator of TPC2 activity and shows its role in regulating melanoma cell migration through interactions with RAB7a.

NAADP elicits two-pore channel currents by lifting Lsm12-mediated inhibition of PI(3,5)P2 activation

This study shows NAADP reverses Lsm12 inhibition of PI(3,5)P2-dependent TPC activation, enabling Ca2+ release from acidic organelles.

Domain-Specific Agonist Binding Affinities Explain Structural and Functional Regulation of TRPM2

This study quantifies the binding affinities of TRPM2 domains for ADPR analogs and shows that MHR1/2 drives activation while NUDT9H maintains structural integrity.

Activation of TPC2 amplifies lysosome-mitochondria calcium transfer to regulate energetic stress responses

This study shows TPC2 activation enhances lysosome-mitochondria calcium transfer, affecting mitochondrial function and stress responses, with implications for neuroprotection in stroke.

Transient signaling of free ADP-ribose monitored with an intracellular biosensor

The paper develops a biosensor to measure intracellular ADP-ribose dynamics and finds that ADPR levels are transient, tunable, and critical for TRPM2 activation.

Mechanoresilience of lysosomes conferred by TMEM63A

This study identifies TMEM63A as a mechanosensitive channel in lysosomes that relieves membrane tension by expelling cations and osmoticants, preventing rupture and cell death.

Liquid liquid phase separation of the intrinsically disordered protein JPT2compartmentalizes components of NAADP-evoked Ca2+ signaling

This study shows JPT2, an intrinsically disordered NAADP-binding protein, undergoes liquid-liquid phase separation, forming condensates that recruit components involved in NAADP-evoked Ca2+ signaling.

Lysosomal LRRC8 complex regulates lysosomal pH, morphology and systemic glucose metabolism

This study shows that the lysosomal LRRC8 complex regulates lysosomal pH, morphology, and systemic glucose metabolism by affecting mTOR signaling and autophagic flux.

SARM1, the executioner of axon degeneration, is an ADP-ribosyl transferase and autoMARylation negatively regulates its activation

This study identifies SARM1 as an ADP-ribosyl transferase that auto-MARylates, regulating its activity and subcellular localization in axon degeneration.

TRPML1 signaling at lysosomes-mitochondria nexus drives triple-negative breast cancer mitophagy, metabolic reprogramming and chemoresistance

This study identifies TRPML1 as a key regulator of mitochondrial function and chemoresistance in TNBC by modulating lysosomal-mitochondria signaling and autophagy.

RNF13 mediates pH- and Ca2+-dependent regulation of lysosomal positioning

This study identifies RNF13 as a regulator of lysosomal positioning, showing its activity is controlled by pH and Ca2+ levels through interactions with ALG-2 and ARL8B.

TRPML3 regulates neuronal gene expression in an in vitro model of autophagy and may act as a genetic marker of familial neurodegenerative disorders

The paper investigates TRPML3's role in autophagy-induced gene expression changes in neuronal cells and suggests it may serve as a genetic marker for neurodegenerative disorders.

The lysosomal carrier SLC29A3 supports anti-bacterial signaling and promotes autophagy by activating TRPML1 in mouse dendritic cells

This study shows SLC29A3 in mouse dendritic cells supports anti-bacterial signaling and autophagy by activating TRPML1, which promotes cytokine production and inflammation control.

TMEM165 replenishes lysosomal calcium stores, protects cells against calcium overload, and mediates calcium-induced lysosomal H+ leakage

This study identifies TMEM165 as a lysosomal calcium importer that prevents calcium overload and regulates lysosomal pH.

IP3R2 mediated inter-organelle Ca2+ signaling orchestrates melanophagy

This paper identifies IP3R2 as a negative regulator of melanophagy, showing it controls melanosome degradation through Ca2+ signaling and lysosomal pathways.

Two-pore channel protein 2-mediated calcium release promotes angiopoietin 2 secretion by regulating Rab46-dependent Weibel-Palade body trafficking

The paper shows that TPC2-mediated Ca2+ release regulates Rab46-dependent Weibel-Palade body trafficking, promoting Ang2 secretion in endothelial cells.

Perilysosomal Ca2+ overload impairs autophagic degradation in β-cell lipotoxicity

This study shows that perilysosomal Ca2+ overload impairs autophagy in β-cells under lipotoxic stress, and restoring Ca2+ homeostasis can improve β-cell survival.

Lysosomal calcium signalling contributes to the acute α-adrenergic response via calcium-stimulated adenylyl cyclase 1 and 8

This study shows that lysosomal calcium signaling via NAADP and calcium-stimulated adenylyl cyclase 1 and 8 contributes to the acute α-adrenergic response in atrial myocytes.