B. Koch

A general method for the development of multicolor biosensors with large dynamic ranges

The paper introduces a method to create multicolor biosensors with large dynamic ranges using FRET pairs based on fluorescent proteins and HaloTag.

Live-cell fluorescence lifetime multiplexing using synthetic fluorescent probes

The paper demonstrates that eight pairwise combinations of 18 synthetic fluorescent probes are suitable for fluorescence lifetime multiplexing in mammalian cells, enabling imaging of up to six biological targets.

HaloTag9: an engineered protein tag to improve fluorophore performance

HaloTag9 improves fluorophore performance with higher brightness and fluorescence lifetime, enabling live-cell multiplexing and biosensor generation.

A quantitative map of nuclear pore assembly reveals two distinct mechanisms

The paper maps nuclear pore complex assembly in human cells, revealing two distinct molecular mechanisms with different orders of component addition.

Mps2 links Csm4 and Mps3 to form a telomere-associated LINC complex in budding yeast

The paper identifies Mps2 as a mediator forming a heterotrimeric telomere-associated LINC complex in budding yeast meiosis, linking Csm4 and Mps3.

Synergistic Material-Topography Combinations to Achieve Immunomodulatory Osteogenic Biomaterials

The paper identifies synergistic material-topography combinations that direct hMSC fate and modulate macrophage phenotype for bone regeneration.

The thrombopoietin receptor agonist eltrombopag inhibits human cytomegalovirus replication via iron chelation

This paper shows eltrombopag inhibits HCMV replication via iron chelation, effective against drug-resistant strains and synergistic with ganciclovir.

A general strategy to develop cell permeable and fluorogenic probes for multi-colour nanoscopy

The paper develops a strategy to create fluorogenic probes for live-cell nanoscopy with high cell permeability and low background signal.

Absolute quantification of cohesin, CTCF and their regulators in human cells

The paper measures absolute copy numbers and dynamics of cohesin, CTCF, and related proteins in human cells, revealing that some genomic enrichment sites are unoccupied in single cells.

The anaphase-promoting complex regulates the degradation of the inner nuclear membrane protein Mps3 in budding yeast

This study shows that the APC/C complex regulates Mps3 degradation at the inner nuclear membrane in budding yeast, linking protein quality control to nuclear morphology and cell division.

A quantitative map of human Condensins provides new insights into mitotic chromosome architecture

The paper quantifies Condensin I and II dynamics during mitosis, proposing a three-step loop model for chromosome compaction.