A. Sali

The Molecular Architecture of the Nuclear Basket

This study uses structural biology to reveal the architecture of the nuclear basket in yeast and mammals, showing how Mlp/Tpr proteins form its structural components.

Integrative mapping reveals molecular features underlying the mechanism of nucleocytoplasmic transport

The paper constructs an integrative Brownian dynamics model of nucleocytoplasmic transport, revealing how FG repeat proteins and nuclear transport receptors mediate selective transport through NPCs.

Multi-scale photocatalytic proximity labeling reveals cell surface neighbors on and between cells

The paper introduces a multi-scale proximity labeling method using Eosin Y to map cell surface protein neighborhoods at varying resolutions and validates interactions of EGFR and CAR-T cell synapses.

Bayesian Fluorescence Framework for integrative modeling of biomolecules

The paper introduces BFF, a Bayesian framework for processing fluorescence data to improve biomolecular modeling accuracy and precision using synthetic single-molecule FRET data.

Unique Binding and Stabilization Mechanisms Employed By and Engineered Into Nanobodies.

This study identifies mechanisms enabling nanobodies to bind antigens with high affinity and stability, revealing a conserved region critical for their function and suggesting strategies for their optimization.

Illuminating the Function of the Orphan Transporter, SLC22A10 in Humans and Other Primates

This study identifies SLC22A10 as a nonfunctional pseudogene in humans due to a missense mutation, while great ape orthologs retain functional uptake of estradiol-17β-glucuronide.

Nanobody repertoire generated against the spike protein of ancestral SARS-CoV-2 remains efficacious against the rapidly evolving virus

The paper shows that nanobodies against ancestral SARS-CoV-2 spike protein remain effective against recent variants, including omicron sublineages and emerging XBB/BQ.1.1 variants.

Dynamic molecular mechanism of the nuclear pore complex permeability barrier

The paper investigates the dynamic behavior of the nuclear pore complex permeability barrier in yeast, showing it functions as a dynamic polymer brush rather than a phase-separated condensate.

Structure and dynamics of the essential endogenous mycobacterial polyketide synthasePks13

This paper determines the structure and dynamics of the mycobacterial polyketide synthase Pks13 using cryoEM and complementary techniques, revealing intermediate states and substrate binding.

Structural Basis of Prostaglandin Efflux by MRP4

This study determines the cryo-EM structures of MRP4 bound to prostaglandins and DHEA-S, revealing its unique substrate specificity and transport mechanism.

Comprehensive Structure and Functional Adaptations of the Yeast Nuclear Pore Complex

This study presents the structure of the yeast nuclear pore complex using cryo-EM, revealing its functional adaptations and evolutionary links.

Bayesian metamodeling of complex biological systems across varying representations

This paper introduces Bayesian metamodeling to integrate diverse models of biological systems, demonstrating its application to glucose-stimulated insulin secretion in pancreatic β-cells.

Assembly and levels of P-TEFb depend on reversiblephosphorylation of cyclinT1

The paper shows that P-TEFb assembly and levels depend on reversible phosphorylation of cyclinT1 by PKC and dephosphorylation by PP1, leading to P-TEFb disassembly and CycT1 degradation in quiescent and terminally differentiated cells.

A quantitative map of nuclear pore assembly reveals two distinct mechanisms

The paper maps nuclear pore complex assembly in human cells, revealing two distinct molecular mechanisms with different orders of component addition.

The YPhi Motif Defines the Structure-Activity Relationships of Human 20S Proteasome Activators

The paper identifies the Y-F/Y (Y{Phi}) motif as the human 20S proteasome recognition sequence, revealing structural and functional differences from archaeal systems.

Nanobody Repertoires for Exposing Vulnerabilities of SARS-CoV-2

This paper presents a large repertoire of high-affinity nanobodies against SARS-CoV-2 Spike protein with potent neutralization properties and resistance to viral variants.

Doublecortin engages the microtubule lattice through a cooperative binding mode involving its C- terminal domain

This study reveals how doublecortin binds to microtubules through a cooperative mechanism involving its C-terminal domain and C-tails, with implications for neurological disorders.

CM1-driven assembly and activation of Yeast γ-Tubulin Small Complex underlies microtubule nucleation

This study elucidates the role of the CM1 motif in Spc110p in facilitating γ-Tubulin Small Complex (γTuSC) assembly and γ-Tubulin Ring Complex (γTuRC) formation in yeast.

Structural Basis of CD4 Downregulation by HIV-1 Nef

This paper presents a high-resolution crystal structure of HIV-1 Nef interacting with AP2, revealing how Nef sequesters CD4 for degradation and identifying a dual-function pocket crucial for both MHC recruitment and CD4 binding.