K. Walker

Pollen Partners: The Symbiotic Microbes of Pinus radiata Pollen

This study characterizes the microbiome of Pinus radiata pollen, revealing a fungal-dominated community that is stable across locations and years, with some bacterial taxa showing geographic and temporal variation.

Development of Ingenui-T, a Novel Vein-to-Vein Solution for Rapid Autologous CAR T-Cell Manufacturing Starting From Whole Blood, for the Treatment of Autoimmune Diseases

This paper introduces Ingenui-T, a method for rapid CAR T-cell manufacturing from whole blood, showing comparable or better functional activity than conventional methods.

Endangered Przewalski's horse, Equus przewalskii, cloned from historically cryopreserved cells

The paper reports the cloning of two endangered Przewalski's horse stallions from cryopreserved fibroblast cells, marking the first successful production of multiple healthy clones for an endangered species.

Plant Cellulose as a Substrate for 3D Neural Stem Cell Culture

This study shows that plant-derived cellulose scaffolds support NSC attachment, proliferation, and enhance differentiation into neuronal and astrocytic lineages compared to 2D culture.

Comprehensive assessment of functional effects of commonly used sweeteners on ex vivo human gut microbiome

This study evaluates the functional effects of 21 common sweeteners on human gut microbiomes using metaproteomics, identifying potential prebiotic properties in certain sugar alcohols.

Evaluating live microbiota biobanking using an ex vivo microbiome assay and metaproteomics

This study evaluates the cultivability and functional activity of biobanked microbiota using an ex vivo assay and metaproteomics, showing stability after long-term freezing.

Examining the effects of an anti-Salmonella bacteriophage preparation, BAFASAL, on ex vivo human gut microbiome composition and function using a multi-omics approach

This study evaluates the impact of BAFASAL, a bacteriophage preparation, on human gut microbiome composition and function using multi-omics analyses in an ex vivo setting.

Exploring the microbiome-wide lysine acetylation, succinylation and propionylation in human gut microbiota

The paper develops a method to analyze lysine acylations in the human gut microbiome, identifying thousands of modified peptides and showing differential distribution across microbial species.

Transcriptomic analysis of naïve human embryonic stem cells cultured in three-dimensional PEG scaffolds

This study shows that 3-D PEG scaffolds maintain the naive state of human ESCs and alter gene expression compared to 2-D culture.

Indigenous plants promote insect biodiversity in urban greenspaces

This study shows that indigenous plants, especially graminoids, support higher insect biodiversity in urban greenspaces compared to nonnative species.

Studying the dynamics of the gut microbiota using metabolically stable isotopic labeling and metaproteomics.

The paper develops a metaproteomic workflow and software tool to study gut microbiome functional dynamics using stable isotopic labeling and time series analysis.

Growth phase-dependent responses of an in vitro gut microbiome to metformin

This study shows that metformin affects in vitro gut microbiota differently depending on when it is added during growth phases, with the log phase showing the strongest impact on growth and functional changes.

An in vitro model maintaining taxon-specific functional activities of the gut microbiome

The paper develops an in vitro model called Mipro that maintains gut microbiome functional and compositional profiles, showing strong correlation with in vivo responses to metformin.

Therapeutic Targeting of Casein Kinase 1δ/ε in an Alzheimer's Disease Mouse Model

This study shows that inhibiting CK1δ/ε improves memory and circadian rhythms in Alzheimer's mice by reversing proteomic changes and synaptic plasticity.