This study generates hiPSC-derived myofibres with the m.3243A>G mutation to model mitochondrial disease, showing metabolic and physiological changes related to mutation load.

bioRxiv · Jun 11, 2024 · PreprintCell Biology

Human pluripotent stem cell-derived hepatocyte-like cells for hepatitis D virus studies

H. Chi, B. Qu, A. Prawira, L. Maurer, J. Hu, R. M. Fu, F. A. Lempp, Z. Zhang, D. Grimm, X. Wu, S. Urban, V. L. Dao Thi

This study shows that hepatocyte-like cells derived from human pluripotent stem cells are permissive to hepatitis D virus infection and can be used to study HDV biology and test therapies.

bioRxiv · Oct 13, 2023 · PreprintMicrobiology

Antiviral Type III CRISPR signalling via conjugation of ATP and AdoMet

H. Chi, V. Hoikkala, S. Gruschow, S. Graham, S. Shirran, M. F. White

This study identifies SAM-AMP as a novel second messenger in type III-B CRISPR systems, used for antiviral signaling by conjugating ATP to S-adenosyl methionine.

bioRxiv · Jun 26, 2023 · PreprintMicrobiology

Flavivirus prM interacts with MDA5 and MAVS to inhibit RLR antiviral signaling

L. Sui, Y. Zhao, W. Wang, H. Chi, T. Tian, P. Wu, J. Zhang, Y. Zhao, Z.-K. Wei, Z. Hou, G. Zhou, G. Wang, Z. Wang, Q. Liu

This study identifies how flavivirus prM proteins interact with MDA5 and MAVS to inhibit RLR antiviral signaling, revealing species-dependent immune evasion mechanisms.

bioRxiv · Apr 25, 2022 · PreprintMicrobiology

A subset of Memory B-derived antibody repertoire from 3-dose vaccinees is ultrapotent against diverse and highly transmissible SARS-CoV-2 variants, including Omicron

K. Wang, Z. Jia, L. Bao, L. Wang, L. Cao, H. Chi, Y. Hu, Q. Li, Y. Jiang, Q. Zhu, Y. Deng, P. Liu, N. Wang, L. Wang, M. Liu, , X. Wang

Antibodies from 3-dose vaccinated individuals neutralize Omicron and other variants, with some being ultrapotent and broadly reactive.

bioRxiv · Dec 24, 2021Microbiology

SAVED by a toxin: Structure and function of the CRISPR Lon protease.

C. Rouillon, N. Schneberger, H. Chi, M. F. Peter, M. Geyer, W. Boenigk, R. Seifert, M. F. White, G. Hagelueken

The paper reports the crystal structure of CRISPR-Lon, a protein that activates by binding cA4 and cleaves CRISPR-T to release a nuclease-like fragment.

bioRxiv · Dec 6, 2021Biochemistry

Exploring the microbiome-wide lysine acetylation, succinylation and propionylation in human gut microbiota

X. Zhang, K. Cheng, Z. Ning, J. Mayne, K. Walker, H. Chi, C. Farnsworth, K. Lee, D. Figeys

The paper develops a method to analyze lysine acylations in the human gut microbiome, identifying thousands of modified peptides and showing differential distribution across microbial species.

bioRxiv · Dec 14, 2020Microbiology

SARS-CoV-2 envelope protein causes acute respiratory distress syndrome (ARDS)-like pathological damage and constitutes an antiviral target

Z. Gao, B. Xia, X. Shen, Y. He, X. Pan, Y. Wang, F. Yang, S. Fang, Y. Wu, X. Zuo, Z. Xie, X. Jiang, H. Chi, Q. Meng, H. Zhou, , J. Li

This study shows the SARS-CoV-2 envelope protein causes ARDS-like damage and identifies channel inhibitors as potential antiviral targets.

bioRxiv · Jun 27, 2020Microbiology