M. Zerial

Hep3D: A 3D single-cell digital atlas of the liver to study spatio-temporal tissue architecture

The paper develops Hep3D, a 3D single-cell atlas of the mouse liver, revealing spatiotemporal patterns of cellular structures and the structural role of Kupffer cells in liver homeostasis.

GTPase-dependent cyclic flexibility transitions drive the two-component EEA1-Rab5 molecular motor

This study shows that Rab5 and EEA1 form a two-component molecular motor using GTP hydrolysis to drive cyclic flexibility transitions that perform mechanical work.

Anisotropic expansion of hepatocyte lumina enforced by apical bulkheads

This study identifies apical bulkheads in hepatocytes as mechanical structures that drive anisotropic lumen expansion during liver development.

Quantitative intracellular retention of delivered RNAs through optimized cell fixation and immuno-staining

The paper optimizes cell fixation and immuno-staining to quantitatively retain and visualize exogenously delivered and endogenous nucleic acids in sub-cellular compartments.

Structure of the human FERRY Rab5 effector complex

The paper determines the cryo-EM structure of the human FERRY Rab5 effector complex and shows Fy-2 acts as a binding hub for subunits and mRNA/Rab5.

The novel Rab5 effector FERRY links early endosomes with the translation machinery

This study identifies FERRY as a Rab5 effector that localizes mRNAs and ribosomes to early endosomes, impacting mRNA distribution and causing brain damage when disrupted.

Endosomal escape of delivered mRNA from endosomal recycling tubules visualized at the nanoscale

The paper visualizes mRNA escape from endosomal recycling tubules using super-resolution microscopy and identifies parameters affecting endosomal escape and cytotoxicity in human cells.

Bridging scales in scattering tissues via multifocal two-photon microscopy

This paper introduces multifocal two-photon microscopy to image mesoscopic scattering tissues at high resolution and speed without tissue-clearing methods.

A non-linear system patterns Rab5 GTPase on the membrane

The paper demonstrates that Rab5 GTPase forms membrane patterns through a handover mechanism involving Rabex5/Rabaptin5 and requires an endosomal lipid composition.

Correlative SMLM and electron tomography reveals endosome nanoscale domains

This paper develops a superCLEM workflow combining SMLM and electron tomography to visualize endosome nanoscale domains in HeLa cells, revealing Rab5 organization distinct from cargo molecules.

3D spatially-resolved geometrical and functional models of human liver tissue reveal new aspects of NAFLD progression

This study creates 3D spatial models of human liver tissue to identify new morphometric parameters and topological defects linked to NAFLD progression.

Allosteric structural alterations and auto-regulation of Rab5 GEF activity in Rabex5

This study reevaluates the autoinhibition mechanism of Rabex5 by analyzing structural changes and nucleotide exchange activity, revealing new regulatory roles for Ubiquitin binding domains.

Retrograde transport of Akt by a neuronal Rab5-APPL1 endosome

The paper identifies Rab5-APPL1 endosomes as mediators of retrograde Akt1 transport in hippocampal pyramidal neurons.

Liquid-crystal organization of liver tissue

The paper reveals that hepatocytes in liver tissue follow a long-range liquid-crystal order, challenging the traditional view of random organization and highlighting bi-directional communication between hepatocytes and sinusoids.

Rab5 and Alsin regulate stress-activated cytoprotective signaling on mitochondria

The paper identifies a novel pathway involving Rab5 and Alsin that regulates stress-activated cytoprotective signaling on mitochondria, linking mitochondrial stress response to ALS pathology.