W.-D. Hardt

Intraluminal neutrophils limit epithelium damage by reducing pathogen assault on intestinal epithelial cells during Salmonella gut infection

This study shows that intraluminal neutrophils protect the intestinal epithelium from Salmonella-induced damage by reducing pathogen load and physical blockage during infection.

Salmonella cancer therapy metabolically disrupts tumours at the collateral cost of T cell immunity

This study shows that Salmonella-based cancer therapy impairs T cell metabolism, which is linked to its anti-tumor effects.

Gasdermin D is the only Gasdermin that provides non-redundant protection against acute Salmonella gut infection

This study identifies Gasdermin D as the only Gasdermin providing non-redundant protection against acute Salmonella Typhimurium gut infection by restricting bacterial loads, controlling inflammation, and preventing epithelial disruption.

The mobilizable plasmid P3 of Salmonella enterica serovar Typhimurium SL1344 depends on the P2 plasmid for conjugative transfer into a broad range of bacteria in vitro and in vivo

Vaccine-enhanced competition permits rational bacterial strain replacement in the gut

The paper demonstrates that oral vaccination combined with engineered competitor strains can exclude pathogens and replace bacterial strains in the mouse gut.

Fitness advantage of Bacteroides thetaiotaomicron capsular polysaccharide is dependent on the resident microbiota

This study quantifies colonization bottlenecks and growth dynamics of Bacteroides thetaiotaomicron in the gut, showing that capsular polysaccharide provides a fitness advantage dependent on microbiota composition.

Metabolic reconstitution by a gnotobiotic microbiota varies over the circadian cycle

This study shows that a gnotobiotic microbiota affects metabolic processes differently depending on the circadian cycle, influencing energy balance and substrate utilization.

A rationally designed oral vaccine induces Immunoglobulin A in the murine gut that directs the evolution of attenuated Salmonella variants

This paper shows that an oral vaccine inducing IgA in mice can direct Salmonella to evolve into less virulent variants with shorter O-antigens.

Pathogen invasion-dependent tissue reservoirs and plasmid-encoded antibiotic degradation boost plasmid spread in the gut

This study shows that plasmid dissemination in the gut is enhanced by antibiotic degradation in the lumen and cycling between gut and tissue reservoirs in Salmonella Typhimurium.

Microbiota-derived metabolites inhibit Salmonella virulent subpopulation development by acting on single-cell behaviors

This study shows microbiota-derived short-chain fatty acids reduce Salmonella virulent subpopulation frequency by slowing their growth, not by repressing SPI-1 expression at the single-cell level.

Cooperative virulence can emerge via horizontal gene transfer but is stabilized by transmission

This study shows that horizontal gene transfer can enable virulence in Salmonella Typhimurium, but cooperative virulence is stabilized by transmission dynamics and population bottlenecks.

Clinical extended-spectrum beta-lactamase antibiotic resistance plasmids have diverse transfer rates and can spread in the absence of antibiotic selection

This study quantifies the conjugative transfer dynamics of ESBL plasmids in vitro and in vivo, showing variation in transfer efficiency and potential for predicting plasmid spread.

Enchained growth and cluster dislocation: a possible mechanism for microbiota homeostasis

The paper investigates how IgA antibodies affect bacterial growth and clustering in the gut, finding that faster replicating bacteria are more affected by IgA-mediated chain breaking.

Microbiota stability in healthy individuals after single-dose lactulose challenge - a randomized controlled study

This study examines the effect of a single-dose lactulose challenge on gut microbiota stability and E. coli density in healthy individuals, finding no significant changes in E. coli levels or microbiota composition.