E. Entcheva

OptoDyCE-plate as an affordable high throughput imager for all optical cardiac electrophysiology

OptoDyCE-plate enables high-throughput optical cardiac electrophysiology in 96- and 384-well plates, showing drug response variability and pacing rate effects.

CRISPRi Gene Modulation and All-Optical Electrophysiology in Post-Differentiated Human iPSC-Cardiomyocytes

The paper uses CRISPRi and all-optical electrophysiology to study gene modulation effects in post-differentiated human iPSC-CMs, finding specific phenotype changes in cardiac ion channels.

High-throughput optical sensing of peri-cellular oxygen in cardiac cells: system characterization, calibration, and testing

This paper develops and validates a high-throughput system for long-term optical measurement of peri-cellular oxygen in cardiac cells, showing dynamic oxygen changes and differences between hiPSC-CMs and fibroblasts.

Simultaneous widefield voltage and interferometric dye-free optical mapping quantifies electromechanical waves in human iPSC-cardiomyocytes.

This study develops a method to simultaneously measure voltage and mechanical waves in human iPSC-CMs using dye-free optical imaging, revealing new insights into electromechanical coupling and its sensitivity to pharmacological agents.

Portable low-cost macroscopic mapping system for all-optical cardiac electrophysiology

The paper introduces a low-cost system for macroscopic all-optical cardiac electrophysiology in hiPSC-CMs, showing equivalency between electrical and optogenetic stimulation and demonstrating utility for studying cellular uncoupling agents.

Sex-dependent transcriptional control of cardiac electrophysiology by histone acetylation modifiers based on the GTEx database

The paper uses GTEx data to show sex-specific effects of histone modifiers on cardiac ion channels via transcription factors, with male models underestimating female effects.

INTEGRATION OF ENGINEERED "SPARK-CELL" SPHEROIDS FOR OPTICAL PACING OF CARDIAC TISSUE

This study engineers 'spark-cell' spheroids to enable optical pacing of cardiac tissue, showing they can stimulate human stem-cell-derived cardiomyocytes faster than direct genetic modification.

Optogenetic currents in myofibroblasts acutely alter electrophysiology and conduction of co-cultured cardiomyocytes

This study shows that optogenetic activation of myofibroblasts in co-cultures with cardiomyocytes reduces conduction velocity and shortens action potential duration, indicating electrical coupling between cell types.

Microfluidics-enabled 96-well perfusion system for high-throughput tissue engineering and long-term all-optical electrophysiology

This paper introduces a high-throughput microfluidics system for perfusion and validates its use in long-term all-optical electrophysiology of human cells.

Hysteresis in calcium rhythm patterns during electric field stimulation of cardiac tissue

The paper investigates hysteresis in calcium rhythm patterns during electric field stimulation of cardiac tissue, revealing transitions between instability patterns and providing insights into their rules.

Syncytium cell growth increases IK1 contribution in human iPS-cardiomyocytes

The paper shows that higher density cultures of hiPS-CMs increase Ik1 expression, leading to more stable electrophysiology and reduced spontaneous activity.

All-optical electrophysiology refines populations of in silico human iPS-CMs for drug evaluation

This paper combines all-optical electrophysiology with in silico models to refine human hiPS-CMs for drug evaluation, showing accurate prediction of drug effects and adverse events.

Optical interrogation of sympathetic neuronal effects on macroscopic cardiac monolayer dynamics

This study uses optical methods to investigate how sympathetic neurons affect cardiac monolayer dynamics, focusing on neurotransmitter release, neuronal density effects, and optogenetics applications.

Multimodal on-axis platform for all-optical electrophysiology with near-infrared probes in human stem-cell-derived cardiomyocytes

The paper introduces an all-optical platform for high-throughput electrophysiology in human stem-cell-derived cardiomyocytes using optogenetic stimulation and optical imaging.

Adeno-associated virus mediated gene delivery: Implications for scalable in vitro and in vivo cardiac optogenetic models

This study compares AAV serotypes 1, 6, and 9 for cardiac optogenetic applications, finding AAV1/6 are more effective in vitro while AAV9 is superior in vivo due to receptor differences.

OptoGap: an optogenetics-enabled assay for quantification of cell-cell coupling in multicellular cardiac tissue

The paper introduces OptoGap, a method to quantify cell-cell coupling in cardiac tissue by optogenetically stimulating nonexcitable cells and measuring the response in coupled excitable cells.