W. Peng

An emerging PB2-627 polymorphism increases the pandemic potential of avian influenza virus by breaking through ANP32 host restriction in mammalian and avian hosts

This paper identifies a PB2-627V mutation in avian influenza viruses that enhances pandemic potential by overcoming host restriction through ANP32A proteins in both avian and mammalian hosts.

LRRK2 G2019S mutation suppresses differentiation of Th9 and Treg cells via JAK/STAT3

This study shows that the LRRK2 G2019S mutation impairs Th9 and Treg cell differentiation while enhancing Th2 differentiation through JAK/STAT3 signaling in T cells.

Architecture of the spinach plastid-encoded RNA polymerase

This study presents the cryo-EM structure of the spinach plastid-encoded RNA polymerase complex, revealing its composition and spatial arrangement of subunits and associated proteins.

FASlpr gene dosage tunes the extent of lymphoproliferation and T cell differentiation in lupus

This study shows that FASlpr gene dosage affects lymphoproliferation and T cell differentiation in lupus models, with heterozygous Faslpr leading to increased autoantibodies and Th1 cell skewing.

HIV-1 binds dynein directly to hijack microtubule transport machinery

This study shows HIV-1 directly binds dynein to hijack microtubule transport for motility, bypassing traditional cargo adaptors.

Multiple genetic variants at the SLC30A8 locus affect local super-enhancer activity and influence pancreatic β cell survival and function

The paper identifies multiple genetic variants at the SLC30A8 locus that influence enhancer activity and β cell function, with some variants affecting gene expression and insulin secretion.

Reverse engineering the anti-MUC1 hybridoma antibody 139H2 by mass spectrometry-based de novo sequencing

This paper reverse engineers the anti-MUC1 hybridoma antibody 139H2 using mass spectrometry-based sequencing and validates its properties through various assays.

Direct mass spectrometry-based detection and antibody sequencing of Monoclonal Gammopathy of Undetermined Significance from patient serum - a case study.

This paper detects MGUS using LC-MS and determines M-protein sequences from serum, providing molecular detail for monoclonal gammopathies.

The lung employs an intrinsic surfactant-mediated inflammatory response for viral defense

This study shows that surfactant proteins mediate an intrinsic inflammatory response in the lung to defend against SARS-CoV-2 infection.

Engineering non-cytotoxic delivery of proteins by T cells via fusion to NPC2

This study demonstrates that fusing proteins to NPC2 enables non-cytotoxic protein delivery by T cells through the perforin pathway.

The ChlorON Series: Turn-on Fluorescent Protein Sensors for Imaging Labile Chloride in Living Cells

The paper introduces the ChlorON series of fluorescent protein sensors for imaging labile chloride in living cells with a turn-on response at physiological pH.

Discovery of a monomeric green fluorescent protein sensor for chloride by structure-guided bioinformatics

The paper identifies a new chloride-sensitive fluorescent protein, GFPxm163, through bioinformatics and validates its properties for cellular imaging.

Minor differences in peptide presentation between chicken MHC class I molecules can explain differences in disease susceptibility

The paper shows that differences in peptide binding between chicken MHC class I alleles BF2*1901 and BF2*1501 explain their differing susceptibility to Mareks disease.

Glycan shield of the ebolavirus envelope glycoprotein GP

This study profiles the site-specific glycosylation patterns of ebolavirus GP using mass spectrometry, revealing complex N-, O-, and C-linked glycans that contribute to the glycan shield and restrict antibody neutralization.

Salmonella enterica serovar Typhimurium chitinases modulate the intestinal glycome and promote small intestinal invasion.

This study shows Salmonella chitinases enhance intestinal invasion by modulating host glycans, with chitinase-deficient strains showing reduced invasion and delayed dissemination.

Double nicking by RNA-directed Cascade-nCas3 for high-efficiency large-scale genome engineering

This paper introduces a CRISPR-nCas3 system for efficient genome editing in bacteria, enabling high-efficiency deletion of large genomic regions.

Structure-based design of a highly stable, covalently-linked SARS-CoV-2 spike trimer with improved structural properties and immunogenicity

This paper designs a modified SARS-CoV-2 spike trimer, VFLIP, with enhanced stability, immunogenicity, and structural fidelity to the native spike.

Mass spectrometry-based sequencing of the anti-FLAG-M2 antibody using multiple proteases and a dual fragmentation scheme

This paper introduces a method for de novo sequencing of monoclonal antibodies using multiple proteases and dual fragmentation, achieving high accuracy in sequence coverage.

Evidence of Superior and Inferior Sinoatrial Nodes in the Mammalian Heart

The paper identifies two distinct sinoatrial nodes in mammalian hearts, sSAN and iSAN, which control fast and slow heart rates under sympathetic and parasympathetic stimulation, respectively.

Discovery of the first selective M4 muscarinic acetylcholine receptor antagonists with in vivo anti-parkinsonian and anti-dystonic efficacy

This paper discovers the first selective M4 muscarinic acetylcholine receptor antagonists and confirms their anti-parkinsonian and anti-dystonic efficacy in rodent models.

Structures of an intact yeast V-ATPase alone and in complex with bacterial effector VopQ

This study reports cryo-EM structures of the intact yeast V-ATPase and its complex with the bacterial effector VopQ, revealing interactions and catalytic conformations.

Single-cell transcriptome profiling of an adult human cell atlas of 15 major organs

The paper constructs an adult human cell atlas from 84,363 cells across 15 organs, identifying 252 cell subtypes and revealing inter- and intra-organ heterogeneity.

The common neoantigens in colorectal cancer are predicted and validated to be presented or immunogenic

The paper predicts and validates common neoantigens in colorectal cancer that are both presented and immunogenic, identifying potential universal targets for immunotherapy.

Fluorescence biosensor for real-time interaction dynamics of host proteins with HIV-1 capsid tubes

This paper develops a fluorescence biosensor to quantify host protein interactions with HIV-1 capsid tubes in real-time using total internal reflection fluorescence microscopy.

Characterization and repurposing of the endogenous Type I-F CRISPR-Cas system of Zymomonas mobilis for genome engineering

This study characterizes and repurposes the endogenous Type I-F CRISPR-Cas system of Zymomonas mobilis to create an efficient genome engineering toolkit with 100% efficiency in various genetic modifications.

High-resolution cryo-EM structures of the E. coli hemolysin ClyA oligomers

This study determines high-resolution cryo-EM structures of E. coli hemolysin ClyA oligomers, revealing new oligomeric states and mechanistic insights into pore assembly.