K. J. Pienta

Multiparameter flow cytometric detection and analysis of rare cells in in vivo models of cancer metastasis

The paper introduces a flow cytometry method to detect and analyze rare CTCs and DTCs in cancer metastasis models, enabling the study of metastatic barriers across multiple steps in a single model system.

MYC-driven increases in mitochondrial DNA copy number occur early and persist throughout prostatic cancer progression

The paper develops a method to spatially quantify mitochondrial DNA copy number in cancer tissues and finds increased mtDNAcn in prostate cancer progression, linked to MYC activity.

Exploration of Mechanisms of Drug Resistance in a Microfluidic Device and Patient Tissues

This study uses a microfluidic device to induce drug resistance in TNBC cells and identifies the NUPR1/HDAC11 axis as a key factor in chemoresistance, correlating high Nupr1 expression with poor clinical outcomes.

Nuclear morphology predicts cell survival to cisplatin chemotherapy

This study identifies nuclear morphology and functional characteristics associated with cancer cell survival after cisplatin chemotherapy, highlighting the role of the polyaneuploid cancer cell (PACC) state in treatment resistance.

Cells in the Polyaneuploid Cancer Cell (PACC) state have increased metastatic potential

The paper identifies Polyaneuploid Cancer Cells (PACCs) as metastasis-competent cells through increased motility, elasticity, and survival in circulation.

Characterization of extracellular vesicles and artificial nanoparticles with four orthogonal single-particle analysis platforms

This study compares four single-particle analysis platforms for sizing, counting, and phenotyping extracellular vesicles and synthetic particles, identifying differences in performance across modalities.