L. S. Qi

This study develops a frugal CRISPR kit for K-12 education, enabling hands-on genome engineering experiments without traditional lab equipment or high costs.

This study uses super-resolution microscopy to reveal the nanoscale organization of SARS-CoV-2 replication organelles, showing distinct vgRNA and dsRNA clusters and changes over infection stages.

The paper introduces casmini-tool, a database for designing dCasMINI guide RNAs with off-target prediction and PAM profile analysis for CRISPRi/a applications.

This study shows SARS-CoV-2 evades NK cell killing by downregulating NKG2D ligands via Nsp1, reducing NK cell cytotoxicity against infected cells.

This study uses CRISPR/Cas9 to knockout Ndrg2 in dendritic cells, enhancing their regenerative capacity for wound healing in mouse models.

This paper develops a CRISPRi/a approach to reactivate FOXP3 and silence XIST from XCI in human breast cancer cells, showing enhanced epigenetic changes and therapeutic potential.

This study uses multi-color super-resolution imaging to analyze the spatial organization of coronavirus RNA during infection and assess antiviral drug effects.

The paper introduces Dropception, a method for high-throughput single-cell encapsulation and phenotyping using double emulsion picoreactors and FACS.

This paper develops a CRISPR-Cas13-based strategy called PAC-MAN to inhibit SARS-CoV-2 and influenza A virus in human cells, showing it can degrade viral sequences and reduce replication.

This study uses CRISPRi to systematically analyze coding and non-coding elements in E. coli, revealing conditional essentiality and novel insights into promoter and TFBS targeting.

This paper introduces LiveFISH, a method to visualize real-time chromosomal rearrangements in living cells, and demonstrates its application in detecting gene-editing induced translocations.

This study shows that mechanotransduction in breast cancer occurs without YAP, due to compact nuclear morphologies in vivo.