M. Sauer

Traction Force Microscopy with DNA FluoroCubes

This paper introduces FluoroCubes as fiducial markers for traction force microscopy, enabling high-resolution force mapping with improved sensitivity and compatibility with TIRF microscopy.

Decoding the molecular interplay of endogenous CD20 and Rituximab with fast volumetric nanoscopy

The paper develops TDI-DNA-PAINT and LLS microscopy to study CD20-rituximab interactions in B cells, revealing oligomeric states and polarization effects.

Continuous endosomes form functional subdomains and orchestrate rapid membrane trafficking in trypanosomes

The paper investigates the endosomal system in Trypanosoma brucei, revealing it as a continuous membrane system rather than distinct compartments, enabling rapid membrane trafficking.

CARs are organized in nanodomains in the plasma membrane of T cells that accumulate at tumor contact sites

This study uses super-resolution microscopy to show CARs form nanodomains on T-cell membranes, accumulating at tumor sites, with transposon-engineered CARs showing higher density and faster anti-tumor activity.

Trifunctional linkers enable improved visualization of actin by expansion microscopy

The paper introduces TRITON trifunctional linkers to improve actin visualization in expansion microscopy, achieving better signal retention and anchoring.

ROCKETS - a novel one-for-all toolbox for light sheet microscopy in drug discovery

ROCKETS provides a standardized protocol for processing and imaging mouse tissues with LSFM, enabling comprehensive biodistribution analysis of immunotherapies.

Visualizing proteins by expansion microscopy

This paper introduces ONE microscopy, a technique that enables nanoscale imaging of single proteins and molecular complexes, revealing structural details in cells and disease samples.

Sub-10 nm fluorescence imaging

This study identifies challenges in achieving sub-10 nm resolution in fluorescence imaging and proposes a method combining time-resolved detection and photoswitching fingerprint analysis to overcome these challenges.

Isotropic Three-Dimensional Dual-Color Super-Resolution Microscopy with Metal-Induced Energy Transfer

The paper combines MIET imaging with dSTORM to achieve isotropic 3D super-resolution and dual-color imaging of sub-cellular structures.

Super-Resolution Imaging by Dual Iterative Structured Illumination Microscopy

This paper introduces diSIM, a dual iterative structured illumination microscopy technique that improves 3D multicolor super-resolution imaging in fixed and living cells with enhanced spatiotemporal resolution.

Bioorthogonal labeling of transmembrane proteins with non-canonical amino acids allows access to masked epitopes in live neurons

The paper develops a method to label and image masked epitopes of transmembrane proteins in live neurons using non-canonical amino acids and genetic code expansion.

Structural basis for broad coronavirus neutralization

This study identifies a monoclonal antibody that broadly neutralizes multiple beta-coronaviruses by targeting a conserved epitope, providing a structural basis for designing pan-coronavirus vaccines.

Multiple-labeled antibodies behave like single emitters in photoswitching buffer

This study examines how the degree of labeling affects the blinking behavior of multiple-labeled antibodies in dSTORM imaging, finding that spatial resolution remains consistent regardless of labeling.

Confocal Laser-Scanning Fluorescence-Lifetime Single-Molecule Localisation Microscopy

This paper introduces FL-SMLM, a technique combining FL-CLSM and SMLM to achieve fluorescence-lifetime super-resolution imaging with single-molecule localization.

Nanoscale imaging of bacterial infections by sphingolipid expansion microscopy

This study uses sphingolipid expansion microscopy to image bacterial infections at nanoscale resolution, revealing details of intracellular bacterial membranes previously only accessible via electron microscopy.

Using Expansion Microscopy to visualize and characterize the morphology of mitochondrial cristae

This paper uses expansion microscopy to visualize mitochondrial cristae morphology and localize proteins within the intermembrane space using a MtCK-GFP marker.

Molecular resolution imaging by post-labeling expansion single molecule localization microscopy (Ex-SMLM)

This paper introduces post-labeling expansion single molecule localization microscopy (Ex-SMLM) to overcome limitations in super-resolution imaging by preserving ultrastructural details and improving labeling efficiency.

Tracking down the molecular architecture of the synaptonemal complex by expansion microscopy

The paper uses expansion microscopy combined with structured illumination microscopy to study the molecular structure of the synaptonemal complex with high resolution.

Bioorthogonal labeling with tetrazine-dyes forsuper-resolution microscopy

This study explores the use of tetrazine-dyes in super-resolution microscopy via Diels-Alder reactions with TCO-modified ncAAs, showing enhanced fluorescence and specific labeling capabilities.

Imaging beyond the super-resolution limits using ultrastructure expansion microscopy (UltraExM)

This paper introduces UltraExM, a method that enables subdiffraction-resolution imaging of preserved ultrastructures using standard optical microscopy, revealing new insights into centriolar structures.

Boosting The Localization Precision Of dSTORM By Biocompatible Metal-Dielectric Coated Glass Coverslips

This paper improves dSTORM localization precision by using biocompatible metal-dielectric coated glass coverslips, enhancing signal-to-noise ratio by a factor of two.