This paper uses Dynamic Light Scattering to assess phage bioactivity changes over time and optimize storage conditions for clinical phage applications.

bioRxiv · Jul 2, 2023Biophysics

Bacteriophage populations mirror those of bacterial pathogens at sites of infection

N. L. Haddock, L. J. Barkal, P. L. Bollyky

The paper analyzes cell-free DNA from infected bodily fluids to show that bacteriophage sequences mirror the abundance of their bacterial hosts at infection sites.

bioRxiv · May 17, 2023Microbiology

Theranostic gold in a gold cage nanoparticle for photothermal ablation and photoacoustic imaging of skin and oral infections

M. Hajfathalian, C. R. de Vries, J. C. Hsu, A. Amirshaghaghi, Y. C. Dong, Z. Ren, Y. Liu, Y. Huang, Y. Li, S. Knight, P. Jonnalagadda, A. Zlitni, E. Grice, P. L. Bollyky, H. Koo, D. P. Cormode

This study develops a theranostic gold nanoparticle for detecting and treating biofilm infections using photothermal ablation and photoacoustic imaging, showing superior efficacy against oral and wound infections compared to chlorhexidine.

bioRxiv · May 5, 2023 · PreprintBioengineering

FOXP3+ regulatory T cells use heparanase to access IL-2 bound to ECM in inflamed tissues

H. A. Martinez, I. Koliesnik, G. Kaber, N. Nagy, G. Barlow, B. A. Falk, J. K. Reid, C. O. Medina, A. Hargil, I. Vlodavsky, J.-p. Li, M. Perez-Cruz, S.-W. Tang, E. H. Meyer, L. E. Wrenshall, , H. F. Kuipers

The paper shows FOXP3+ Treg use heparanase to access IL-2 bound to ECM in inflamed tissues, enhancing their function and stability.

bioRxiv · Feb 26, 2023Immunology

Pf Bacteriophage Inhibits Neutrophil Migration in the Lung

M. C. Popescu, N. Pennetzdorfer, A. Hargil, G. Kaber, P. L. Bollyky

The paper shows that Pf4 phage inhibits neutrophil migration in the lung by reducing CXCL5 production during bacterial infection.

bioRxiv · Oct 12, 2022 · PreprintImmunology

The Impact of Pf Bacteriophages on the Fitness of Pseudomonas aeruginosa; A Mathematical Modeling Approach

J. D. Pourtois, M. J. Kratochvil, Q. Chen, N. L. Haddock, E. B. Burgener, G. A. De Leo, P. L. Bollyky

This paper uses a mathematical model to show that Pf+ Pseudomonas aeruginosa strains can outcompete Pf- strains under specific antibiotic conditions, promoting antibiotic tolerance.

bioRxiv · Aug 30, 2020 · PreprintMicrobiology

Hyaluronan Synthesis Inhibition Impairs Antigen Presentation and Delays Transplantation Rejection

P. L. Marshall, N. Nagy, G. Kaber, G. L. Barlow, A. Ramesh, B. J. Xie, M. H. Linde, N. L. Haddock, C. A. Lester, Q.-L. Tran, C. R. de Vries, A. Malkovskiy, I. Gurevich, H. Martinez, H. F. Kuipers, , P. L. Bollyky

This study shows that inhibiting hyaluronan synthesis with 4MU impairs antigen presentation and delays transplant rejection in mice and human T-cell reactions.

bioRxiv · Aug 28, 2020Immunology

Filamentous Bacteriophage Delay Healing of Pseudomonas-Infected Wounds

M. S. Bach, C. R. de Vries, A. Khosravi, J. M. Sweere, M. Popescu, A. Hargil, J. D. Van Belleghem, G. Kaber, Q. Chen, E. B. Burgener, D. Liu, Q.-L. Tran, T. Dharmaraj, M. Birukova, V. Sunkari, , P. L. Bollyky

This study shows filamentous bacteriophage delays wound healing in Pseudomonas aeruginosa infections by inhibiting keratinocyte migration, supported by a patient cohort study.

bioRxiv · Mar 10, 2020Microbiology

IL-10 Promotes Endothelial Progenitor Cell Driven Wound Neovascularization and Enhances Healing via STAT3.

S. Balaji, e. steen, x. wang, H. V. Vangapandu, N. Templeman, A. J. Blum, C. M. Moles, H. Li, d. a. narmoneva, T. m. Crombleholme, m. j. butte, p. l. bollyky, s. g. keswani

This study shows IL-10 promotes wound healing by recruiting endothelial progenitor cells through STAT3-dependent regulation of VEGF and SDF-1 in both control and diabetic wounds.

bioRxiv · Sep 8, 2019 · PreprintPhysiology