W. Huber

Multimodal and spatially resolved profiling identifies distinct patterns of T-cell infiltration in nodal B-cell lymphoma entities

Restraining Wnt activation and intestinal tumorigenesis by a Rab35 dependent GTPase relay

This study identifies Rab35 as a tumor suppressor that modulates Wnt signaling through a GTPase relay, highlighting its role in restraining intestinal tumorigenesis.

A single-sample workflow for joint metabolomic and proteomic analysis of clinical specimens

The paper introduces a single-sample workflow, MTBE-SP3, for simultaneous proteomic and metabolomic analysis, showing it reduces technical variation and enables consistent results across biological matrices.

A single-cell multi-omic and spatial atlas of B cell lymphomas reveals differentiation drives intratumor heterogeneity

This study reveals that cellular differentiation drives intratumor heterogeneity in B-cell lymphomas, with distinct maturation states associated with different survival outcomes.

Proteomic profiling identifies Serpin B9 as mediator of resistance to CAR T-cell and bispecific antibody treatment in B-cell lymphoma

High-throughput profiling of drug interactions in Gram-positive bacteria

This study systematically tests drug combinations in Gram-positive bacteria, identifying many new synergies and antagonisms, including effective combinations against drug-resistant strains.

Deep thermal proteome profiling for detection of proteoforms and drug sensitivity biomarkers

The paper uses thermal proteome profiling to detect proteoforms and drug sensitivity biomarkers in acute lymphoblastic leukemia cell lines.

Pre-analytical processing of plasma and serum samples for combined proteome and metabolome analysis

This study evaluates pre-analytical factors affecting plasma and serum proteome and metabolome stability, recommending standardized protocols for clinical use.

PhD and postdoc training outcomes at EMBL: changing career paths for life scientists in Europe.

This study analyzes the career outcomes of 2284 EMBL PhD and postdoc alumni from 1997 to 2020, showing 55% in academic research, 15% in industry, and 15% in science-related professions, with increased competition for PI roles but continued success in research careers.

Comparing the value of mono- versus coculture for high-throughput compound screening in hematological malignancies

This study compares monoculture and stromal coculture for drug screening in hematological malignancies, finding that coculture reduces drug efficacy for many compounds but increases it for JAK inhibitors.

Combinatorial drug-microenvironment interaction mapping reveals cell-extrinsic drug resistance mechanisms and clinically relevant patient subgroups in CLL

This paper maps drug-microenvironment interactions in CLL to identify resistance mechanisms and patient subgroups, revealing how microenvironmental stimuli and genetic alterations influence drug response.

SAMHD1 mutations in mantle cell lymphoma are recurrent and confer in vitro resistance to nucleoside analogues

Dissecting intratumor heterogeneity of nodal B cell lymphomas on the transcriptional, genetic, and drug response level

This study examines intratumor heterogeneity in B cell lymphomas by analyzing transcriptional, genetic, and drug response profiles across malignant subclones and immune cells.

Biological Plasticity Rescues Target Activity in CRISPR Knockouts

The paper quantifies residual protein expression in CRISPR-Cas9 knockouts using RNA-seq and SPS-MS3, identifying mechanisms of partial protein preservation and functional retention.

Proteome thermal stability reflects organ physiology and identifies drug-target engagement in vivo

This study introduces tissue-TPP and blood-CETSA to measure proteome thermal stability, revealing organ physiology and drug-target engagement in vivo.