A. Honigmann

Generic comparison of lumen nucleation and fusion in epithelial organoids with and without hydrostatic pressure

The paper compares lumen nucleation and fusion dynamics in three epithelial organoid systems under varying hydrostatic pressure conditions, revealing conserved self-organisation rules.

Quantitative imaging of species-specific lipid transport in mammalian cells

The paper quantifies lipid transport between organelles in mammalian cells, revealing non-vesicular transport as a key mechanism for species-specific lipid sorting.

Predicting Organoid Morphology Through a Phase Field Model: Insights into Cell Division and Lumenal Pressure

This paper uses a phase field model to predict organoid morphology by simulating cell division and lumenal pressure, identifying key parameters that determine shape and structure.

Cell-substrate distance fluctuations of confluent cells enable fast and coherent collective migration

The paper examines how cell-substrate distance fluctuations affect collective migration in confluent monolayers of MDCKII cells and their ZO1/2-depleted variants.

Intra-condensate demixing of TDP-43 inside stress granules generates pathological aggregates

This study shows that TDP-43 aggregation in stress granules requires up-concentration and oxidative stress, leading to intra-condensate demixing and pathological aggregates.

Assembly of tight junction belts by surface condensation and actin elongation

The paper investigates how surface phase transitions control protein condensation to form tight junction belts through ZO-scaffold proteins and actin polymerization.

Tight junctions regulate lumen morphology via hydrostatic pressure and junctional tension

This study investigates how tight junctions regulate lumen morphology through hydrostatic pressure and junctional tension in MDCKII cysts.

Polarity-driven three-dimensional spontaneous rotation of a cell doublet

This study investigates how myosin polarity drives spontaneous 3D rotation in cell doublets embedded in an extracellular matrix.

Wetting of junctional condensates along the apical interface promotes tight junction belt formation

The paper shows that PATJ-mediated wetting of junctional condensates at the apical membrane drives the formation of tight junction belts.

Dynamic matrices with DNA-encoded viscoelasticity for advanced cell and organoid culture

This paper introduces DyNAtrix, a DNA-encoded hydrogel that allows precise control over viscoelastic properties for 3D cell and organoid cultures.

Reciprocal regulation between cell mechanics and ZO-1 guides tight junction assembly and epithelial morphogenesis

This study shows that ZO-1 regulates cell mechanics and tight junction assembly, with reciprocal effects on epithelial morphogenesis and junction integrity.

Cellular segregation in co-cultures driven by differential adhesion and contractility on distinct time scales

This study investigates how differential adhesion and contractility drive time-dependent segregation in epithelial co-cultures, showing contractility dominates short-term sorting while adhesion determines the final state.

Tight junction ZO proteins maintain tissue fluidity, ensuring efficient collective cell migration

This study shows ZO proteins maintain tissue fluidity and control proliferation to enable efficient collective cell migration.

Optimization of mouse embryonic stem cell culture for organoid and chimeric mice production.

This study compares four mESC culture conditions, finding that CHIR99021/LIF suspension culture best balances effort, pluripotency, and homogeneity for organoid and chimeric mouse production.

Phase separation of zonula occludens proteins drives formation of tight junctions

This study shows ZO proteins undergo phase separation to form tight junctions by creating membrane-bound compartments that organize junction components.

Transcription organizes euchromatin similar to an active microemulsion

The paper shows transcriptionally inactive euchromatin moves away from active euchromatin, forming RNA-enriched microenvironments that resemble an active microemulsion.

Expansion stimulated emission depletion microscopy (ExSTED)

The paper combines expansion microscopy with STED to achieve 30-fold higher resolution than conventional microscopy, enabling ultra-structural imaging at <10 nm lateral and ~50 nm isotropic.