X. Zhao

Deep Profiling of Plasma Proteoforms with Engineered Nanoparticles for Top-down Proteomics

This study uses engineered nanoparticles to enrich low-abundant proteins in human plasma, enabling top-down proteomics to detect proteoforms at lower concentrations and expand proteome depth.

PCM1 conveys centrosome asymmetry to polarized endosome dynamics in regulating daughter cell fate

This study shows that Pcm1 regulates asymmetric endosome dynamics and RGP fate by coordinating Rab5b and Rab11a endosome markers and Par-3/dynein complexes in zebrafish and human brain organoids.

TLCellClassifier: Machine Learning Based Cell Classification for Bright-Field Time-Lapse Images

TLCellClassifier improves cell detection, tracking, and classification in time-lapse images using CNN, metric learning, and LSTM, outperforming existing tools in accuracy and efficiency.

Potent and broadly neutralizing antibodies against sarbecoviruses elicited by single ancestral SARS-CoV-2 infection

This study identifies two broadly neutralizing antibodies against SARS-CoV-2 and related sarbecoviruses from individuals exposed only to ancestral SARS-CoV-2.

FAST-STEM: A human pluripotent stem cell engineering toolkit for rapid design-build-test-learn development of human cell-based therapeutic devices

FAST-STEM accelerates human pluripotent stem cell engineering by enabling rapid transgene integration and differentiation into multiple cell types.

Androgen receptor activity inversely correlates with immune cell infiltration and immunotherapy response across multiple cancer lineages

The paper examines the relationship between androgen receptor activity and immune cell infiltration, showing that lower AR activity correlates with increased immune activity and better immunotherapy response across multiple cancers.

Discovery and Synthesis of GS-7682, a Novel Prodrug of a 4'-CN-4-Aza-7,9-Dideazaadenosine C-Nucleoside with Broad-Spectrum Potency Against Pneumo- and Picornaviruses and Efficacy in RSV-Infected African Green Monkeys.

This paper describes the discovery and synthesis of GS-7682, a prodrug with broad antiviral activity against RSV and other viruses, showing efficacy in RSV-infected monkeys.

Caspase-8-mediated CYLD cleavage boots LPS-induced endotoxic shock.

This study identifies Caspase-8-mediated CYLD cleavage as a mechanism promoting LPS-induced endotoxic shock and reveals CP25 as a potential biomarker.

Biologically informed machine learning modeling of immune cells to reveal physiological and pathological aging process

The paper constructs a transcriptomic atlas of immune cells across human lifespan and develops a biological age prediction model using scRNA-seq and bulk RNA-seq data.

Cryo-electron tomographic investigation of native hippocampal glutamatergic synapses

The paper develops methods to image glutamatergic synapses in hippocampal tissue using cryo-electron tomography, enabling high-resolution visualization of synaptic structures.

Granzyme B-based CAR T cells block metastasis by eliminating circulating tumor cells

This study develops GrB-CAR T cells targeting mHSP70 to eliminate CTCs and inhibit metastasis in solid tumors.

Insights into Cellular Evolution: Temporal Deep Learning Models and Analysis for Cell Image Classification

This study compares temporal deep learning models for classifying sequences of cell colony images to understand cellular state transitions.

Role of Hepatocyte RIPK1 in Maintaining Liver Homeostasis during Metabolic Challenges

This study identifies RIPK1 in hepatocytes as critical for maintaining liver homeostasis during metabolic challenges, showing its role in preventing inflammation and apoptosis in mice.

HER2 Prognostic Significance and Therapeutic Implications in Gastric Cardia Adenocarcinoma: Insights from Multi-Omics Profiling

This study identifies HER2 as a prognostic marker in GCA and suggests anti-inflammatory and immune checkpoint inhibition as therapeutic strategies for HER2-negative patients.

SUMO Promotes DNA Repair Protein Collaboration to Support Alterative Telomere Lengthening in the Absence of PML

This study shows SUMO promotes DNA repair protein collaboration for telomere maintenance in ALT cancer cells without PML bodies.

Architecture and flexibility of native kinetochores revealed by structural studies utilizing a thermophilic yeast

This study reveals the architecture and flexibility of native kinetochores using structural analyses of purified kinetochores from thermophilic yeast.

High-Resolution Spatial Transcriptomic Atlas of Mouse Soleus Muscle: Unveiling Single Cell and Subcellular Heterogeneity in Health and Denervation

Schlafen-11 and -9 are innate immune sensors for intracellular single-stranded DNA

The paper identifies Schlafen-11 and -9 as innate immune sensors that recognize intracellular single-stranded DNA containing CGT motifs, leading to cytokine expression and cell death.

Potent and broadly neutralizing antibodies against sarbecoviruses induced by sequential COVID-19 vaccination

This study identifies potent and broadly neutralizing antibodies against SARS-CoV-2 and related sarbecoviruses from a vaccinated donor, showing their efficacy against multiple variants and providing insights for therapeutic development.

3D Bioprinting of Human Neural Tissues with Functional Connectivity

This study develops a 3D bioprinting platform to create human neural tissues with functional connectivity, showing differentiation into neurons and astrocytes and forming functional circuits.

Control of ciliary transcriptional programs during spermatogenesis by antagonistic transcription factors

This study identifies XAP5 and XAP5L as antagonistic transcription factors that regulate ciliary gene expression during spermatogenesis, with XAP5 promoting and XAP5L inhibiting ciliary transcription factors at different stages.

Genomic signatures associated with the evolutionary loss of egg yolk in parasitoid wasps

This study identifies genomic signatures of yolk loss in parasitoid wasps, showing independent Vg gene loss events and associated functional biases in related genes.

An ancient transcription activator is required for the multiciliogenesis program

This study identifies Edf1 as an essential transcription activator for multiciliogenesis, showing its role in ciliary gene expression and its impact on hydrocephalus in mice.

Nuclear cytoophidia assembly represses transcriptional activity to control skeletal development and homeostasis

This study identifies nuclear cytoophidia as regulators of Impdh2 transcriptional activity, impacting skeletal development and homeostasis through effects on endochondral ossification and osteoclast function.

Single phage proteins sequester TIR- and cGAS-generated signaling molecules

This study identifies Tad1 and Tad2 as phage proteins that sequester cyclic nucleotides produced by TIR and cGAS systems to inhibit anti-phage immunity.

Continuity of Mitochondrial Budding: Insights from BS-C-1 Cells by in-situ Cryo-Electron Tomography

The paper uses in-situ Cryo-ET to study mitochondrial budding in BS-C-1 cells, finding no Drp1 or ER involvement and identifying new mitochondrial-vesicle interactions.

Induction of immortal-like and functional CAR-T cells by defined factors

This study identifies factors that induce CAR-T cells with stem-like properties, enhancing their long-term persistence and antitumor efficacy in vivo.

One infusion of engineered long-lasting and multifunctional T cells cures asthma in mice

This paper shows that engineered T cells can cure asthma in mice by targeting eosinophils and blocking inflammatory cytokines.

Molecular mechanism of NAD+ binding to the Nudix homology domains of DBC1

This study investigates how NAD+ binds to DBC1's Nudix homology domains using NMR, ITC, and molecular docking, revealing key residues and binding affinity.

Functional specialization of cytochrome b5 prior to the invention of syringyl lignin biosynthesis

The study shows that cytochrome b5 D (CB5D) evolved before the divergence of land plant lineages and specialized to support F5H in syringyl lignin biosynthesis.