Author

J. Lescar

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 5 Microbiology· 5 Plant Biology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 9 Research Focus:host-pathogen & immune response· 6 Assay:proteomics· 5 Assay:structural biology· 4 Research Focus:drug response / drug discovery· 3 Organism:plant (other)· 3 Organism:human· 2 Approach:mechanistic / dynamical model· 2

Frequent Co-Authors

A. El Sahili· 4 shared B. C. Goh· 3 shared J. Lin· 3 shared F. Samsudin· 2 shared G. Yue· 2 shared J. P. Tam· 2 shared K. Purushotorman· 2 shared M. K. McQueen· 2 shared

Activity by Year

2020-2022 · peak 5/yr

Co-Author Collaboration Network

10 top collaborators · 16 mutual co-author links

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4 shared papersBiochemistry

A. El Sahili

Mutual co-authors in cluster

Author profile →

11 papers

Recent·Most cited

Substrate-binding Glycine Residues are Major Determinants for Hydrolase and Ligase Activity of Plant Legumains

X. Hemu, N.-Y. Chan, H. T. Liew, S. Hu, X. Zhang, A. Serra, J. Lescar, C.-F. Liu, J. P. Tam

The paper identifies conserved glycine residues as key determinants distinguishing peptide asparaginyl ligases (PALs) from asparaginyl endopeptidases (AEPs) in plant legumains.

bioRxiv · Sep 26, 2022Plant Biology

Erythrocyte signalling is critical for Plasmodium falciparum invasion

J. J. M. Yong, X. Gao, P. Prakash, S. K. Lai, M. W. Chen, J. J. L. Neo, J. Lescar, H. Y. Li, P. R. Preiser

This study shows that Plasmodium falciparum merozoite invasion depends on host cAMP-Ca2+ signalling, with RH5-Basigin interaction triggering cAMP rise and Ca2+ influx.

bioRxiv · Jul 18, 2022 · PreprintMicrobiology

Engineering SARS-CoV-2 cocktail antibodies into a bispecific format improves neutralizing potency and breadth

Z. Ku, X. Xie, J. Lin, P. Gao, A. E. Sahili, H. Su, Y. Liu, X. Ye, X. Li, X. Fan, B. C. Goh, W. Xiong, H. Boyd, A. E. Muruato, H. Deng, , Z. An

This study engineers bispecific antibodies against SARS-CoV-2, showing that one design outperforms a cocktail in neutralizing activity and breadth against variants.

bioRxiv · Feb 1, 2022 · PreprintMicrobiology

Mapping the allosteric effects that define functional activity of SARS-CoV-2 specific antibodies

N. K. Tulsian, P. V. Raghuvamsi, X. Qian, G. Yue, B. D/O Shunmuganathan, F. Samsudin, Y. H. Wong, L. Jianqing, K. Purushotorman, M. K. McQueen, B. Wang, J. Lescar, C.-I. Wang, G. Anand, P. J. Bond, P. A. MacAry

This study identifies site-specific allosteric effects in SARS-CoV-2 Spike protein induced by different neutralizing antibodies, revealing dynamic features that distinguish weak from strong neutralizers.

bioRxiv · Dec 27, 2021 · PreprintBiochemistry

Structural basis for substrate recognition, ligation and activation by a hyperactive Asn peptide ligase from Viola yedoensis

J. Lescar, S. Hu, A. El Sahili, S. Kishore, X. Hemu, C. F. Liu, J. P. Tam, B. C. Goh, Y. H. Wong, Z. Wang

The paper captures VyPAL2, a plant peptide ligase with a bound substrate, revealing the molecular basis for substrate recognition and ligation.

bioRxiv · Dec 9, 2021 · PreprintBiochemistry

Identification of an inhibitory pocket in falcilysin provides a new avenue for malaria drug development

G. Wirjanata, J. Lin, J. Dziekan, A. El Sahili, Z. Chung, S. Tjia, N. E. B. Zulkifli, J. Boentoro, R. Tham, S. J. Lai, K. Go, Y. Han, A. Partridge, D. Olsen, N. Prabhu, , Z. Bozdech

This study identifies falcilysin as a target of anti-malaria drugs and reveals a shared inhibitory pocket that could guide new drug development.

bioRxiv · Apr 8, 2021Microbiology

SARS-CoV-2 S protein ACE2 interaction reveals novel allosteric targets

G. S. Anand, R. Palur, N. K. Tulsian, F. Samsudin, X. Qian, K. Purushotorman, G. Yue, M. K. McQueen, J. Lescar, P. Bond, P. A. McAry

The paper investigates how ACE2 binding to SARS-CoV-2 Spike protein allosterically affects proteolysis at distal cleavage sites and highlights potential therapeutic targets.

bioRxiv · Oct 13, 2020Biochemistry

The SiaABC threonine phosphorylation pathway controls biofilm formation in response to carbon availability in Pseudomonas aeruginosa

W.-H. Poh, J. Lin, B. Colley, N. Müller, B. C. Goh, D. Schleheck, A. El Sahili, A. Marquardt, Y. Liang, S. Kjelleberg, J. Lescar, S. A. Rice, J. Klebensberger

This study identifies the SiaABC threonine phosphorylation pathway as a regulator of biofilm formation in Pseudomonas aeruginosa in response to carbon availability and surfactant stress.

bioRxiv · Apr 16, 2020Microbiology

HflX controls hypoxia-induced non-replicating persistence in slow growing mycobacteria

J. Y. G. NGAN, S. PASUNOOTI, W. TSE, W. MENG, S. F. C. NGAN, S. W. NG, M. T. JAAFAR, H. JIA, S. L. S. CHO, J. LIM, H. Q. V. KOH, N. ABDULGHANI, K. PETHE, K. S. Sze, J. Lescar, S. Alonso

This study shows HflX in slow-growing mycobacteria enables hypoxia-induced non-replicating persistence, contributing to drug tolerance and dormancy.

bioRxiv · Mar 13, 2020 · PreprintMicrobiology

Crystal structures of full length DENV4 NS2B-NS3 reveal the dynamic interaction between NS2B and NS3 upon binding to protease inhibitors

W. W. Phoo, A. El Sahili, C. W. Liew, J. Lescar, S. Vasudevan, D. Luo, M. W. Chen

The paper reports crystal structures of DENV4 NS2B-NS3 constructs with and without protease inhibitors, showing how different linkers affect the enzyme's conformation and activity.

bioRxiv · Jan 27, 2020Biochemistry

Structure of a consensus chitin-binding domain revealed by solution NMR

D. Heymann, H. Mohanram, A. Kumar, C. S. Verma, J. Lescar, A. Miserez

The paper determines the 3D structure of a chitin-binding domain from a squid CBP using NMR, revealing how the R&R motif binds chitin and providing insights for biomimetic material design.

bioRxiv · Jan 8, 2020Biochemistry