W. Ouyang

Wireless, Multimodal Monitoring of Organ Health Using 3D-Printed, Barbed, Bioresorbable Stretchable Microneedle Sensor Arrays

This paper introduces a wireless, 3D-printed microneedle sensor array for multimodal organ monitoring, capable of measuring metabolism, oxygenation, and electrophysiology with spatiotemporal resolution.

Artificial Sweeteners in US-Marketed Oral Nicotine Pouch Products: Correlation with Nicotine Contents and Effects on Product Preference

This study identifies artificial sweeteners in US nicotine pouches and shows they increase nicotine consumption and reduce aversion, suggesting they enhance product appeal.

ESPressoscope: a small and powerful platform for in situ microscopy

The paper introduces ESPressoscope, a low-cost, portable digital microscope platform for in situ imaging, demonstrating its versatility and accessibility for various biological and ecological applications.

A multiplexed time-resolved fluorescence resonance energy transfer ultrahigh-throughput screening assay for targeting SMAD4-SMAD3-DNA complex

This paper develops a multiplexed TR-FRET assay to screen small molecule inhibitors targeting the SMAD4-SMAD3-DNA complex, identifying gambogic acid and gambogenic acid as potential hits.

ISG15-modification of the Arp2/3 complex restricts pathogen spread

This study shows ISG15 modifies the Arp2/3 complex to restrict pathogen spread by slowing actin filament formation and stabilizing structures, but pathogens like Listeria exploit dysregulated ISGylation to enhance spread.

Uncovering molecular grammars of intrinsically disordered regions that organize nucleolar fibrillar centers

The paper identifies molecular grammars in intrinsically disordered regions of nucleolar proteins that drive phase separation to form nucleolar substructures.

Subcellular mapping of the protein landscape of SARS-CoV-2 infected cells for target-centric drug repurposing

The paper uses immunofluorescence to analyze host protein changes in SARS-CoV-2 infection and identifies three drugs that reduce antiviral activity.

Functional stemness-related genes revealed by single-cell profiling of naïve and stimulated human CD34+ cells from CB and mPB

This study identifies stemness-related genes in human CD34+ cells from different sources and culture conditions using scRNA-seq, revealing pathways involved in stemness maintenance and potential drug targets for HSC expansion.

An Open-Source Modular Framework for Automated Pipetting and Imaging Applications

This paper presents an open-source framework for automated pipetting and imaging using affordable tools, enabling reproducible and customizable lab experiments.

Variable Induction of Pro-inflammatory Cytokines by Commercial SARS CoV-2 Spike Protein Reagents: Potential Impacts of LPS on In Vitro Modeling and Pathogenic Mechanisms In Vivo

This study shows that commercial SARS CoV-2 spike protein reagents vary in their ability to induce pro-inflammatory cytokines, likely due to contamination with endotoxin (LPS).

DeepImageJ: A user-friendly environment to run deep learning models in ImageJ

DeepImageJ provides a user-friendly environment for using pre-trained deep learning models in ImageJ for biomedical image analysis tasks.

Sequencing of clinical samples reveals that adaptation keeps establishing during H7N9 virus infection in humans

This study analyzes H7N9 virus genome mutations in human clinical samples to identify potential adaptations during infection.

Restoration of β-globin expression with optimally designed lentiviral vector for β-thalassemia treatment in Chinese patients

This paper develops a lentiviral vector for β-thalassemia treatment that restores β-globin expression in Chinese patients with minimal tumorigenesis risk.

Transcriptome analyses of β-thalassemia -28 (A>G) mutation using isogenic cell models generated by CRISPR/Cas9 and asymmetric single-stranded oligodeoxynucleotides (assODN)

This study uses CRISPR/Cas9 and assODN to create an isogenic cell model of the HBB -28 (A>G) mutation and analyzes its transcriptome effects, revealing altered gene expression and pathway activation.

Mapping the nucleolar proteome reveals a spatiotemporal organization related to intrinsic protein disorder

The paper maps the human nucleolar proteome, identifies a new subcompartment, and shows intrinsic disorder levels are higher in nucleolar and mitotic proteins.

Serum-free culture system for spontaneous human mesenchymal stem cell spheroids formation

This study develops a serum-free method to generate human mesenchymal stem cell spheroids using knockout serum replacement, showing similar transcriptomes to FBS-based spheroids and retained MSC markers.