M. Hartl

Single-cell profiling uncovers regulatory programs of pathogenic Th2 cells in allergic asthma

This study uses scRNA-seq to identify two distinct subsets of pathogenic Th2 cells in allergic asthma and establishes an in vitro model to study their regulation.

Targeting the catalytic activity of HDAC1 in T cells protects against experimental autoimmune encephalomyelitis

This study shows that inhibiting HDAC1 catalytic activity in T cells prevents experimental autoimmune encephalomyelitis by reducing proinflammatory cytokines and effector functions.

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

This study shows that lysosome positioning influences PLEKHG3 localization and protrusive activity, but protrusion changes can occur without PLEKHG3.

An improved TurboID pipeline in C. elegans by biochemical depletion of endogenously biotinylated carboxylases

The paper improves TurboID labeling in C. elegans by depleting endogenous biotinylated carboxylases to enhance interactome analysis.

In search of the universal method: a comparative survey of bottom-up proteomics sample preparation methods

The paper compares 16 bottom-up proteomics sample preparation methods to identify optimal strategies for protein extraction and MS analysis.

Unexpected Complexity of the Ammonia Monooxygenase in Archaea

The paper identifies two new subunits, AmoY and AmoZ, in the ammonia monooxygenase of ammonia oxidizing archaea, revealing structural and functional complexity.

Mimicking tumor cell heterogeneity of colorectal cancer in a patient-derived organoid-fibroblast model

This paper develops a colorectal cancer organoid model incorporating patient-derived fibroblasts, showing enhanced cellular heterogeneity and morphology compared to mono-cultures.

The CHK-2 antagonizing phosphatase PPM-1.D regulates meiotic entry via catalytic and non-catalytic activities

The paper identifies PPM-1.D as a phosphatase that antagonizes CHK-2 kinase to regulate meiotic entry through both catalytic and non-catalytic mechanisms.

BacPROTACs mediate targeted protein degradation in bacteria

This paper develops BacPROTACs, small-molecule degraders that target bacterial proteins by activating the ClpC:ClpP protease, demonstrating in vivo degradation in mycobacteria.

Peroxiredoxin promotes longevity and H2O2-resistance in yeast through redox-modulation of PKA

This study shows that Tsa1 promotes longevity and H2O2 resistance in yeast by modulating the PKA pathway through redox modifications.

PP2A-Rts1 antagonizes Rck2-mediated hyperosmotic stress signaling in yeast

The paper identifies PP2A-Rts1 as an antagonist of Rck2-mediated hyperosmotic stress signaling in yeast by showing their interaction and regulation of phosphorylation sites.

Production and Purification of Endogenously Modified tRNA-Derived Small RNAs

This study develops a biochemical method to produce and purify specific tRNA-derived small RNAs from human cells to better understand their molecular functions.

B cells sustain inflammation and predict response to immune checkpoint blockade in human melanoma

Within-patient HIV mutation frequencies reveal fitness costs of CpG dinucleotides, drastic amino acid changes and G->A mutations

The paper estimates the fitness costs of 870 HIV mutations in vivo by analyzing mutation frequencies in the pol gene across 160 patients, finding higher costs for non-synonymous, drastic amino acid changes, and CpG-creating mutations.