M. Herlyn

Spatiotemporal profiling defines persistence and resistance dynamics during targeted treatment of melanoma

This study uses spatial transcriptomics and deep learning to analyze melanoma treatment resistance, identifying persister states and resistance mechanisms with spatiotemporal resolution.

Dasatinib Resensitizes MAPK Inhibitor Efficacy in Standard-of-Care Relapsed Melanomas

The paper identifies dasatinib as effective in restoring BRAFi/MEKi antitumor efficacy in 70% of resistant PDX models by targeting proteins involved in therapy escape.

Persister state-directed transitioning and vulnerability in melanoma

This study identifies a compound that induces a persister state in melanoma cells, promoting differentiation and sensitizing them to a prodrug, offering a dual-target strategy for treatment.

Metastatic potential in clonal melanoma cells is driven by a rare, early-invading subpopulation

The paper identifies a rare invasive subpopulation of melanoma cells that drives metastasis, independent of environment, through non-genetic gene expression fluctuations.

clampFISH 2.0 enables rapid, scalable amplified RNA detection in situ

clampFISH 2.0 enables rapid, scalable detection of multiple RNA species in single cells and tissue sections by improving signal amplification and reducing time and cost.

PDXNet Portal: Patient-Derived Xenograft model, data, workflow, and tool discovery

The paper introduces the PDXNet Portal, a centralized resource for PDX models and data, offering analysis workflows and facilitating cancer research collaboration.

Systematic Establishment of Robustness and Standards in Patient-Derived Xenograft Experiments and Analysis

The paper tests the reproducibility of PDX drug responses and sequence results across different labs using standardized protocols and workflows.

Suppression of p16 increases nucleotide synthesis via mTORC1

This study shows that p16 suppression increases nucleotide synthesis through mTORC1 activation, promoting tumorigenesis by bypassing senescence.

B cells sustain inflammation and predict response to immune checkpoint blockade in human melanoma

Genome-wide prediction of synthetic rescue mediators of resistance to targeted and immunotherapy

The paper predicts synthetic rescue mediators of resistance to targeted and immunotherapy using tumor transcriptomics and survival data from 10,000 TCGA patients, validating predictions through experimental screens.