P. Cramer

The pioneer transcription factor ELF2 remodels the nucleosome near transcription start sites

This study reveals how ELF2, a pioneer transcription factor, remodels nucleosomes near transcription start sites by unwrapping DNA and exposing chromatin, facilitating transcription initiation.

Three-step mechanism of promoter escape by RNA polymerase II

The paper identifies a three-step mechanism for RNA polymerase II promoter escape using in vitro reconstitution and structural analysis.

Epstein-Barr Virus Orchestrates Spatial Reorganization and Immunomodulation within the Classic Hodgkin Lymphoma Tumor Microenvironment

This study uses spatial multi-omics to compare EBV-positive and EBV-negative cHL, identifying distinct TME features and immunological signatures linked to EBV.

A YIPF5-GOT1A/B complex directs a transcription independent function of ATF6 in ER export

The paper identifies YIPF5 and GOT1A/B as components of ER export and shows ATF6 has a transcription-independent role in ER export inhibition by FLI-06.

Visualizing translation dynamics at atomic detail inside a bacterial cell

This study uses cryo-electron tomography to visualize translation dynamics in Mycoplasma pneumoniae, revealing ribosome states and showing how antibiotics affect translation.

Mechanism of molnupiravir-induced SARS-CoV-2 mutagenesis

This paper identifies the molecular mechanism by which molnupiravir induces SARS-CoV-2 RNA mutations through its interaction with the viral RNA-dependent RNA polymerase.

Scaling of cellular proteome with ploidy

The paper investigates how gene expression scales with ploidy in budding yeast and identifies a conserved mTORC1-S6K-Tup1 pathway that regulates proteome remodeling.

Structural basis of nucleosome transcription mediated by Chd1 and FACT

This paper determines cryo-EM structures of Pol II-Spt4/5-nucleosome complexes with bound Chd1 or FACT, revealing their roles in facilitating Pol II passage through nucleosomes.

Mechanism of SARS-CoV-2 polymerase inhibition by remdesivir

This study elucidates how remdesivir inhibits SARS-CoV-2 RNA-dependent RNA polymerase by causing RNA synthesis stalling through a translocation barrier.

Integrator is a genome-wide attenuator of non-productive transcription

The paper identifies genome-wide roles of the Integrator complex in attenuating non-productive transcription by dampening weak promoters and quality-controlling RNAPII complexes.

Multi-particle cryo-EM refinement with M visualizes ribosome-antibiotic complex at 3.7 A inside cells

The paper introduces a software tool, M, for multi-particle cryo-EM refinement that achieves 3.7 Å resolution of a ribosome-antibiotic complex inside bacterial cells.

Structure of replicating SARS-CoV-2 polymerase

This study presents the cryo-EM structure of the SARS-CoV-2 RdRp complex, revealing its mechanism of RNA replication and potential antiviral targets.

Ruler elements in chromatin remodelers set nucleosome array spacing and phasing

The paper identifies a ruler element in chromatin remodelers that determines nucleosome spacing and phasing in Saccharomyces cerevisiae.

Structure of transcribing RNA polymerase II-nucleosome complex

The paper reports the cryo-EM structure of transcribing RNA polymerase II engaged with a nucleosome in yeast, revealing interactions and potential mechanisms for chromatin transcription.

Real-time cryo-EM data pre-processing with Warp

Warp processes cryo-EM data in real-time during acquisition, improving resolution and automating pre-processing steps.