J. Rappsilber

Molecular basis for condensin enrichment at pericentromeres

This study identifies a molecular mechanism by which condensin is recruited to pericentromeres through a conserved interaction between Ycg1 and Sgo1, ensuring proper chromosome segregation.

Molecular basis of mRNA delivery to the bacterial ribosome

This study investigates how bS1 facilitates mRNA delivery to the ribosome and enhances translation initiation by RNAP in bacteria.

PLK1-Mediated Phosphorylation Cascade Activates the Mis18 Complex to Ensure Centromere Inheritance

The paper identifies PLK1-mediated phosphorylation of Mis18 and Mis18BP1 as essential for activating the Mis18 complex to ensure CENP-A loading and centromere inheritance.

Control of iron homeostasis by a regulatory protein-protein interaction in Bacillus subtilis: The FurA (YlaN) acts as an antirepressor to the ferric uptake regulator Fur

The paper identifies FurA as an antirepressor of Fur in Bacillus subtilis, showing it prevents Fur from binding DNA under iron limitation, thus regulating iron uptake genes.

The sterol transporter STARD3 transports sphingosine at ER-lysosome contact sites

The paper identifies STARD3 as a sphingosine transporter at ER-lysosome contact sites, showing its role in sphingosine integration into ceramide.

Direct recruitment of Mis18 to interphase spindle poles promotes CENP-A chromatin assembly

The paper shows that Mis18 directly binds to SPB components to recruit Mis18C, promoting CENP-A chromatin assembly independently of centromeres.

Ki-67 is necessary during DNA replication for forks protection and genome stability

This study shows Ki-67 is necessary for DNA replication, genome stability, and prevents DNA damage and interferon response when depleted.

AI-guided cryo-EM probes a thermophilic cell-free system with succinyl-coA manufacturing capability

The paper characterizes a thermophilic cell-free system producing succinyl-CoA using cryo-EM and biochemical methods, revealing its molecular structure and functional organization.

Structure and mechanism of a novel cytomegaloviral DCAF mediating interferon antagonism

This study identifies E27 as a viral protein that targets STAT2 for degradation via CRL4 ubiquitin ligases, revealing its structural mechanism of interferon antagonism.

Proteasome-dependent truncation of the negative heterochromatin regulator Epe1 mediates antifungal resistance

The paper shows that proteasome-dependent truncation of Epe1 mediates antifungal resistance in fission yeast by altering H3K9 methylation and gene expression.

Visualizing translation dynamics at atomic detail inside a bacterial cell

This study uses cryo-electron tomography to visualize translation dynamics in Mycoplasma pneumoniae, revealing ribosome states and showing how antibiotics affect translation.

The phospho-docking protein 14-3-3 regulates microtubule-associated proteins in oocytes including the chromosomal passenger Borealin

This study identifies microtubule-associated proteins regulated by 14-3-3 in Drosophila oocytes, showing its role in Borealin localization and function through phosphorylation-dependent binding.

Structural Basis for Mis18 Complex Assembly: Implications for Centromere Maintenance

This study characterizes the structure of the Mis18 complex and identifies structural determinants essential for its assembly and centromere maintenance.

Molecular Basis for CPC-Sgo1 Interaction: Implications for Centromere Localisation and Function of the CPC

This study identifies the molecular mechanism of CPC-Sgo1 interaction and shows its role in centromere localization and function through structural and functional analyses.

Mapping the invisible chromatin transactions of prophase chromosome remodelling

The paper maps early chromatin transactions during mitotic entry in chicken cells using proteomics and imaging, revealing dynamic changes in chromatin associations and protein dynamics.

Structural insights into Cullin4-RING ubiquitin ligase remodelling by Vpr from simian immunodeficiency viruses

This paper investigates how SIVmus Vpr interacts with CRL4DCAF1 to ubiquitylate SAMHD1, revealing structural and mechanistic insights into viral counteraction of host restriction factors.

STING Nuclear Partners Contribute to Innate Immune Signalling Responses

The paper identifies nuclear STING partners that contribute to innate immune signaling by interacting with IRF3/7 transcription factors and enhancing antiviral responses.

Non-redundant functions of H2A.Z.1 and H2A.Z.2 in chromosome segregation and cell cycle progression.

This study identifies non-redundant roles of H2A.Z.1 and H2A.Z.2 in cell cycle regulation and chromosome segregation in human cells.

Megadalton-sized dityrosine aggregates of α-synuclein retain high degrees of structural disorder and internal dynamics.

This study examines megadalton-sized dityrosine aggregates of α-synuclein, revealing their structural disorder and dynamics, and their role in inhibiting classical amyloid formation.

The proteomic landscape of centromeric chromatin reveals an essential role for the Ctf19CCAN complex in meiotic kinetochore assembly

The paper uses proteomics to identify meiosis-specific kinetochore remodelling and reveals the essential role of the Ctf19cCCAN complex in meiotic kinetochore assembly.

Isolation of protein N-terminal peptides by charge-mediated position- elective enrichment using strong cation exchange chromatography

The paper introduces a method to enrich and profile protein N-terminal peptides using TrypN digestion and CHAMP-SCX chromatography, achieving high specificity and sensitivity in human cell lysates.

Stress-induced translation inhibition through rapid displacement of scanning initiation factors

The paper shows stress-induced translation inhibition in yeast through rapid displacement of scanning initiation factors and selective mRNA degradation.

The C-terminal helix of BubR1 is essential for CENP-E-dependent chromosome alignment

This study identifies the molecular interaction between BubR1 and CENP-E required for CENP-E recruitment to kinetochores and chromosome alignment during mitosis.

Proteomics Using Protease Alternatives to Trypsin Benefits from Sequential Digestion with Trypsin

The paper evaluates protease alternatives to trypsin for protein identification in S. pombe, showing that sequential digestion with trypsin improves identification rates for several proteases.

Architecture of the Mto1/2 microtubule nucleation complex

The paper investigates the structure and organization of the Mto1/2 complex, revealing its multimeric architecture and the role of specific domains in dimerization.

Structural Basis for CAL1-Mediated Centromere Maintenance

This study reveals how Drosophila CAL1 targets CENP-A to centromeres via structural interactions with CENP-A/H4 and CENP-C, bypassing the need for the Mis18 complex.

Direct Nucleosome Binding of Borealin Secures Chromosome Association and Function of the Chromosomal Passenger complex

This study identifies Borealin as the key mediator of Chromosomal Passenger Complex (CPC) chromosome association through direct nucleosome binding, revealing its critical role in chromosome segregation.

An integrated workflow for cross-linking/mass spectrometry

The paper introduces a workflow combining sequential digestion and Xi software to improve cross-linking mass spectrometry detection of protein interactions.

Sequential digestion with Trypsin and Elastase in cross-linking/mass spectrometry

The paper introduces a sequential digestion method using trypsin and elastase to improve cross-linking/mass spectrometry analysis of protein complexes by accessing previously inaccessible regions.

Defining the RNA Interactome by Total RNA-Associated Protein Purification

This paper introduces TRAPP, a method to identify RNA-binding proteins genome-wide, and demonstrates its application in yeast and bacteria, revealing novel RNA-protein interactions and their changes under stress.