S. Crotty

SARS-CoV-2 monoclonal antibody treatment followed by vaccination shifts human memory B cell epitope recognition suggesting antibody feedback

This study shows that SARS-CoV-2 mAb treatment shifts memory B cell epitope recognition, affecting their response to vaccination.

Early antiviral CD4 and CD8 T cell responses and antibodies are associated with upper respiratory tract clearance of SARS-CoV-2

The paper examines early T cell and antibody responses in COVID-19 patients and finds they correlate with reduced SARS-CoV-2 viral RNA in the upper respiratory tract.

Focusing antibody responses to the fusion peptide in rhesusmacaques

This study uses continuous immunogen delivery in rhesus macaques to focus antibody responses on the HIV fusion peptide, identifying key residues for vaccine design.

Mll1 pioneers histone H3K4me3 deposition and promotes formation of CD8+ T stem cell memory

This study identifies Mll1 as a key factor in establishing CD8+ T stem cell memory by promoting H3K4me3 deposition and regulating Pol II elongation during TCR stimulation.

IL-2 secreting T helper cells promote EF B cell maturation via intrinsic regulation of B cell mTOR/AKT/Blimp-1 axis.

The paper identifies IL-2's role in promoting B cell maturation by regulating the mTOR/AKT/Blimp-1 axis through T helper cell interactions.

Structural basis for antibody-mediated neutralization of Lymphocytic choriomeningitis virus

This study presents the cryoEM structure of LCMV surface glycoprotein and shows that the antibody M28 neutralizes the virus by stabilizing its prefusion state, offering a therapeutic candidate for LCMV infection.

NVX-CoV2373 vaccination induces functional SARS-CoV-2-specific CD4+ and CD8+ T cell responses

This study shows NVX-CoV2373 vaccine induces CD4+ and CD8+ T cell responses, with CD4+ T cells correlating with neutralizing antibody titers.

Humoral and cellular immune memory to four COVID-19 vaccines

This study evaluates immune memory responses to four COVID-19 vaccines over six months, finding stable T and B cell memory but declining neutralizing antibodies with mRNA vaccines.

Omicron BA.1 and BA.2 neutralizing activity elicited by a comprehensive panel of human vaccines

This study evaluates neutralizing activity against Omicron BA.1 and BA.2 variants elicited by various vaccines and finds that booster doses enhance neutralizing titers and breadth across all vaccine platforms.

SARS-CoV-2 vaccination induces immunological memory able to cross-recognize variants from Alpha to Omicron

This study shows that SARS-CoV-2 vaccines induce T cell responses that cross-recognize variants from Alpha to Omicron, with high preservation of CD4+ and CD8+ T cell epitopes.

Long-lasting germinal center responses to a priming immunization with continuous proliferation and somatic mutation

This study shows that germinal center B cells can persist and continue to mutate for 29 weeks after a single immunization, leading to long-lasting antibody responses.

Distinguishing COVID-19 infection and vaccination history by T cell reactivity

This study develops a T cell-based method to distinguish between SARS-CoV-2 infection and vaccination by analyzing T cell reactivity to specific epitopes, achieving high classification accuracy across different groups.

Mucosal and systemic responses to SARS-CoV-2 vaccination in infection naive and experienced individuals

This study examines immune responses to SARS-CoV-2 vaccination in individuals with and without prior infection, finding higher antibody levels and neutralizing titers in previously infected individuals.

Single-cell lineage trajectories and chromatin regulators that initialize antiviral CD8 T cell ontogeny

The paper defines single-cell trajectories and identifies chromatin regulators that establish antiviral CD8 T cell heterogeneity during acute and chronic infections.

Broadly neutralizing antibodies to SARS-related viruses can be readily induced in rhesus macaques

This study shows that rhesus macaques can be immunized to produce broadly neutralizing antibodies against multiple SARS-related viruses, highlighting differences in B cell repertoires between humans and macaques.

From Structure to Sequence: Identification of polyclonal antibody families using cryoEM

This paper uses cryoEM and next-generation sequencing to identify monoclonal antibodies from polyclonal sera and validate their antigen-binding properties.

Vaccine genetics of IGHV1-2 VRC01-class broadly neutralizing antibody precursor naive human B cells

The paper screens naive B cells for VRC01-class broadly neutralizing antibody precursors using a germline-targeting immunogen and single-cell BCR sequencing, finding common germline variation affecting gene usage.

Negligible impact of SARS-CoV-2 variants on CD4+ and CD8+ T cell reactivity in COVID-19 exposed donors and vaccinees.

The paper analyzes T cell responses to SARS-CoV-2 variants and finds minimal impact on CD4+ and CD8+ T cell reactivity in convalescents and vaccinees.

Polyclonal antibody responses to HIV Env immunogens resolved using cryoEM

The paper uses cryoEM to analyze polyclonal antibody responses to HIV Env immunogens in rhesus macaques, revealing structural differences in antibody binding and immunogenicity of engineered mutations and glycan sites.

Comprehensive analysis of T cell immunodominance and immunoprevalence of SARS-CoV-2 epitopes in COVID-19 cases

The paper identifies SARS-CoV-2 epitopes recognized by CD4+ and CD8+ T cells in COVID-19 patients and develops epitope megapools for their detection.

Immunological memory to SARS-CoV-2 assessed for up to eight months after infection

The paper assesses SARS-CoV-2 immune memory components over eight months post-infection, finding varying kinetics in antibodies, B cells, and T cells.

B cells expressing authentic naive human VRC01-class BCRs can be primed and recruited to germinal centers in multiple independent mouse models

The paper demonstrates that authentic naive human VRC01-class BCRs can be primed and recruited to germinal centers in multiple mouse models, validating germline-targeting vaccine design.

Targeting HIV Env immunogens to B cell follicles in non-human primates through immune complex or protein nanoparticle formulations

This study shows that HIV Env immunogens can be concentrated in B cell follicles using immune complexes or protein nanoparticles, enhancing their localization in lymph nodes and potentially improving immune responses.

CD4+ follicular helper-like T cells are key players in anti-tumor immunity

The paper identifies CD4+ follicular helper-like T cells as critical for enhancing anti-tumor CD8+ T cell responses and tumor growth inhibition.

Normal human lymph node T follicular helper cells and germinal center B cells accessed via fine needle aspirations

This study uses lymph node fine needle aspirations to analyze GC B cells and TFH cells in healthy humans, establishing benchmarks for GC biology under noninflammatory conditions.

Vaccine-induced protection from homologous Tier 2 simian-human immunodeficiency virus challenge in nonhuman primates

This study shows that high serum neutralizing antibody titers from Env protein vaccination protect rhesus macaques from Tier 2 SHIV challenge, with ~1:500 ID50 titers providing over 90% protection.

Slow delivery immunization enhances HIV neutralizing antibody and germinal center responses via modulation of immunodominance

This study shows that slow delivery immunization in rhesus macaques increases HIV-neutralizing antibody titers and germinal center responses by modulating immunodominance.

Evidence that recurrent Group A streptococcus tonsillitis is animmunosusceptibility disease involving antibody deficiency and aberrant Tfh cells

The paper investigates recurrent Group A Streptococcus tonsillitis and finds it involves antibody deficiency and aberrant Tfh cells, with genetic factors contributing to susceptibility.