F. Leng

A Fluorescence-Based, T5 Exonuclease-Amplified DNA Cleavage Assay for Discovering Bacterial DNA Gyrase Poisons

This paper introduces a fluorescence-based HTS assay using T5 exonuclease to detect bacterial DNA gyrase poisons, validated with a 50-compound library.

FoxP3 recognizes microsatellites and bridges DNA through multimerization

This study shows FoxP3 forms multimers to bridge DNA through TnG repeats, revealing a new DNA recognition mechanism involving microsatellites.

Anticancer drug doxorubicin spontaneously reacts with GTP and dGTP

This study shows doxorubicin forms covalent conjugates with GTP and dGTP, which are less cytotoxic and cannot enter nuclei, suggesting doxorubicin may target nucleotides involved in cellular energy processes.

FoxP3 can fold into two distinct dimerization states with divergent functional implications for T cell homeostasis

This study shows FoxP3 can form two dimerization states, with head-to-head dimerization supporting function and swap-dimerization impairing it.

Ionic strength modulates HU protein-induced DNA supercoiling

This study investigates how ionic strength affects HU protein-induced DNA supercoiling and compaction using single-molecule techniques.

Negative DNA supercoiling makes protein-mediated looping deterministic and ergodic within the bacterial doubling time

The paper shows that negative DNA supercoiling reduces stochasticity in protein-mediated DNA looping, making it deterministic and ergodic within bacterial doubling time.

Suramin potently inhibits binding of the mammalian high mobility group protein AT-hook 2 to DNA

This study identifies suramin as a potent inhibitor of HMGA2-DNA binding, showing it disrupts HMGA2 function and induces differentiation of brain tumor stem cells.

Mechanism and resistance for antimycobacterial activity of a fluoroquinophenoxazine compound

This study investigates the antimycobacterial mechanism of FP-11g, a fluoroquinophenoxazine compound, and identifies resistance mechanisms in Mycobacterium smegmatis.