A. Leitner

Interaction between ciliary component proteins from Chlamydomonas revealed by CRISPR/CAS9, cryo-electron tomography and mass spectrometry

The paper investigates protein interactions in cilia using CRISPR/CAS9, cryo-ET, and mass spectrometry, revealing a complex involving FAP263, FAP78, FAP184, and FAP151, and links it to dynein f phosphorylation.

Cross-linking mass spectrometric analysis of the endogenous TREX complex from S. cerevisiae

The paper presents the structural analysis of the endogenous TREX complex in S. cerevisiae using cross-linking mass spectrometry and electron microscopy, revealing its dimeric assembly and structural similarities to recombinant complexes.

A structural vista of phosducin-like PhLP2A-chaperonin TRiC cooperation during the ATP-driven folding cycle

This paper reveals the ATP-driven structural rearrangement of PhLP2A during TRiC-mediated protein folding using cryoEM and biochemical analyses.

Regulatory sites of CaM-sensitive adenylyl cyclase AC8 revealed by cryo-EM and structural proteomics

The paper determines the cryo-EM structure of bovine AC8 bound to CaM and Gs, identifies interaction sites, and infers conformational changes in the regulation mechanism.

ROS induction as a strategy to target persister cancer cells with low metabolic activity in NRAS mutated melanoma

The paper identifies ROS induction as a strategy to target MEK-inhibitor resistant NRAS-mutated melanoma cells by exploiting their quiescent metabolic state and elevated baseline ROS levels.

A hybrid structure determination approach to investigate the druggability of the nucleocapsid protein of SARS-CoV-2

The paper develops a hybrid method combining NMR and mass spectrometry to study RNA binding and druggability of SARS-CoV-2 N-protein, identifying potential drug candidates.

Integrative solution structure of a PTBP1-viral IRES complex reveals strong compaction and ordering with residual conformational flexibility

The paper determines the solution structure of a PTBP1-viral IRES complex using integrative modeling, revealing compaction, ordering, and residual flexibility essential for IRES-mediated translation.

A highly sensitive protein-RNA cross-linking mass spectrometry workflow with enhanced structural modelling potential

This paper improves a CLIR-MS workflow for protein-RNA cross-linking, increasing detected products and enabling structural restraint analysis.

Structural visualization of the tubulin folding pathway directed by eukaryotic chaperonin TRiC

The paper visualizes the tubulin folding pathway mediated by TRiC using cryoEM, showing how TRiC's structure guides the folding of β-tubulin through specific interactions.

Molecular architecture of the human tRNA ligase complex

This study presents biochemical and structural analysis of the human tRNA ligase complex, revealing its molecular architecture and catalytic mechanism.

Enhancer RNAs stimulate Pol II pause release by harnessing multivalent interactions to NELF

This study identifies the molecular mechanism by which eRNAs stimulate Pol II pause release by interacting with NELF through unpaired guanosines.

The human GID complex engages two independent modules for substrate recruitment

This study identifies two distinct modules in the human GID complex for substrate recruitment, revealing different roles for WDR26 and GID4 in ubiquitination and substrate recognition.

Insights into autophagosome biogenesis from structural and biochemical analyses of the ATG2A-WIPI4 complex

The paper characterizes the ATG2A-WIPI4 complex's role in autophagosome biogenesis through structural and biochemical analyses.

Integrative structure investigation on the architecture of human Importin4_histone H3/H4_Asf1a complex and its histone H3 tail binding

This study investigates the structure of the Importin4_histone H3/H4_Asf1a complex and identifies key interactions between Importin4 and the histone H3 tail.