J. Hennig

Intrinsically disordered RNA-binding motifs cooperate to catalyze RNA folding and drive phase separation

This study identifies PUN motifs in yeast Loc1p that cooperate to catalyze RNA folding and drive phase separation, suggesting a role in RNA and DNA-related processes across species.

The Drosophila RNA binding protein Hrp48 binds a specificRNA sequence of the msl-2 mRNA 3' UTR to regulatetranslation

This paper identifies the RNA binding specificity of Hrp48, showing it binds the msl-2 mRNA 3' UTR independently of Sxl and Unr downstream of their binding sites.

Structural Insights into The Role of MAGEA4 in RAD18 Regulation: Implications for Ubiquitin Ligase-Binding across the MAGE Protein Family

This study identifies the interaction between MAGEA4 and RAD18 using NMR and AlphaFold2, revealing structural insights into how MAGEA4 regulates RAD18's ubiquitination activity and its implications for cancer.

The Listeria monocytogenes persistence factor ClpL is a potent stand-alone disaggregase

This study identifies ClpL from Listeria monocytogenes as a potent AAA+ disaggregase that enhances heat resistance by efficiently solubilizing protein aggregates under severe stress.

Structural basis of aggregate binding by the AAA+ disaggregase ClpG

The paper determines the structure of the ClpG disaggregase's N1 domain, revealing how it recognizes and binds protein aggregates through hydrophobic interactions and Zn2+ coordination.

Tropomyosin 1-I/C co-ordinates kinesin-1 and dynein motors during oskar mRNA transport

This study shows Tm1-I/C coordinates kinesin-1 and dynein during oskar mRNA transport by inhibiting kinesin-1 until it is needed in the oocyte.

Structural basis of PETISCO complex assembly during piRNA biogenesis in C. elegans

The paper determines the structure of the PETISCO complex in C. elegans and elucidates its role in piRNA biogenesis and early embryogenesis.

The immune Rysto receptor recognizes a broadly conserved feature of potyviral coat proteins

The paper identifies the minimal CP fragment recognized by the Rysto receptor and shows its broad conservation across potyviruses, demonstrating its utility in crop resistance.

Arabidopsis annexin 5 controls plasma membrane properties in mature pollen grains

This study investigates the role of Arabidopsis annexin 5 in maintaining plasma membrane integrity during hydration stress in pollen grains.

Validation and classification of RNA binding proteins identified by mRNA interactome capture

This study validates and classifies RNA binding proteins using NMR and biochemical methods, finding that some novel RBPs interact weakly with RNA and may bind non-specifically.

Mechanistic insights into RNA binding and RNA-regulated RIG-I ubiquitination by TRIM25

This study identifies RNA binding sites on TRIM25 and shows that RNA binding enhances its E3 ligase activity and affects RIG-I ubiquitination.

Transcriptional regulation of the Nε-fructoselysine metabolism in Escherichia coli by global and substrate-specific cues

The paper shows that Escherichia coli regulates fructoselysine metabolism through global and substrate-specific transcriptional regulators to efficiently utilize this compound in the gut.

Spatial accumulation of salicylic acid is regulated by RBOHD in potato immunity against viruses

The paper investigates how RBOHD regulates spatial salicylic acid accumulation in potato immunity against viruses, showing its role in restricting virus spread.

Structure-based screening of binding affinities via small-angle X-ray scattering

This paper demonstrates a method using SAXS to quantify ligand binding affinities without prior structural knowledge, showing its potential for drug discovery.

Structure, dynamics and roX2-lncRNA binding of tandem double-stranded RNA binding domains dsRBD1,2 of Drosophila helicase Maleless

This study determines the NMR structure of MLE's dsRBD1 and dsRBD2 and shows they bind dsRNA independently, but dsRBD1 mutants do not affect roX2 binding or MLE localization.

Extreme resistance to Potato Virus Y in potato carrying the Rysto gene is mediated by a TIR-NLR immune receptor

This study identifies Rysto, a TIR-NLR receptor conferring extreme resistance to PVY in potato, and shows its function requires EDS1 and NRG1 proteins.

Nucleus- and plastid-targeted annexin 5 promotes reproductive development in Arabidopsis and is essential for pollen and embryo formation

This study shows that ANN5 promotes reproductive development in Arabidopsis by regulating pollen and embryo formation through its localization in nuclei, plastids, and interactions with RABE1b.

Structural basis for EarP-mediated arginine glycosylation of translation elongation factor EF-P

The paper determines the crystal structure of EarP, revealing its mechanism for arginine glycosylation of EF-P and its role in bacterial pathogenicity.