C. Lee

A protein interactome for the last eukaryotic common ancestor illuminates the biochemical basis of modern genetic diseases

The paper reconstructs the protein interactome of the Last Eukaryotic Common Ancestor and identifies new human gene-disease associations for bone density and congenital birth defects.

Hidden route of protein damage through confined oxygen gas

The paper identifies a hidden oxidative damage pathway in proteins through O2 confinement and photoexcitation, bypassing antioxidant defenses.

In a nutshell: pistachio genome and kernel development

This study generates a chromosome-scale pistachio genome and analyzes kernel development stages to understand molecular pathways affecting nutritional quality.

Sympathetic neuron-derived NPY protects from obesity by sustaining the mural progenitors of thermogenic adipocytes.

This study shows that sympathetic neuron-derived NPY sustains mural progenitors of thermogenic adipocytes, protecting against obesity by maintaining thermogenic capacity.

Glycogen phase separation drives macromolecular rearrangement and asymmetric division in Escherichia coli

The paper shows glycogen phase separation in E. coli causes asymmetric division and macromolecular rearrangement during growth phase transition.

Spatial proteomic analysis of a lung cancer model reveals regulatory T cells attenuate KRAS-G12C inhibitor-induced immune responses

The paper identifies regulatory T cells as mediators of resistance to KRAS-G12C inhibitors and anti-PD-1 therapy through spatial proteomic analysis of lung cancer models.

How individual P. aeruginosa cells with diverse stator distributions collectively form a heterogeneous macroscopic swarming population

The paper shows that P. aeruginosa cells with different stator distributions create a heterogeneous swarming population through dynamic stator recruitment and motor intermittency.

Global cellular proteo-lipidomic profiling of diverse lysosomal storage disease mutants using nMOST

This study uses nMOST to profile proteo-lipidomics in lysosomal storage disease mutants, revealing autophagy defects and mitochondrial dysfunction.

Kilohertz volumetric imaging of in-vivo dynamics using squeezed light field microscopy

This paper introduces squeezed light field microscopy (SLIM), a method for high-speed 3D volumetric imaging, and demonstrates its application in tracking blood cell motion and neural activity in vivo.

The interplay between electron transport chain function and iron regulatory factors influences melanin formation in Cryptococcus neoformans

This paper investigates how mitochondrial electron transport chain function and iron regulatory factors influence melanin formation in Cryptococcus neoformans, revealing a link between mitochondrial dysfunction, redox homeostasis, and virulence.

Human vascular organoids with a mosaic AKT1 mutation recapitulate Proteus syndrome

This paper creates a vascular organoid model with an AKT1 mutation to study Proteus syndrome and finds AKT inhibitors reverse vascular malformations.

DomainFit: Identification of Protein Domains in cryo-EM maps at Intermediate Resolution using AlphaFold2-predicted Models

DomainFit identifies protein domains in low-resolution cryo-EM maps using AlphaFold2 models, revealing new proteins in cellular complexes.

Paleolithic Gene Duplications Primed Adaptive Evolution of Human Amylase Locus Upon Agriculture

The paper resolves the amylase locus at nucleotide resolution in modern and ancient humans, showing AMY1 copy number variation predated agriculture and was driven by recombination.

Structural basis of aggregate binding by the AAA+ disaggregase ClpG

The paper determines the structure of the ClpG disaggregase's N1 domain, revealing how it recognizes and binds protein aggregates through hydrophobic interactions and Zn2+ coordination.

MiDAS 5: Global diversity of bacteria and archaea in anaerobic digesters

The paper expands the MiDAS database with global anaerobic digester microbiome data, revealing environmental influences on microbial communities and identifying core taxa.

Comparison of SARS-CoV-2 variants of concern in primary human nasal cultures demonstrates Delta as most cytopathic and Omicron as fastest replicating

The paper compares SARS-CoV-2 variants of concern in nasal and lung cultures, finding Omicron replicates fastest and Delta causes most cellular damage.

Heart-on-a-chip model of immune-induced cardiac dysfunction reveals the involvement of free mitochondrial DNA and therapeutic effects of endothelial exosomes

This paper develops a heart-on-a-chip model to study immune-induced cardiac dysfunction, identifying mitochondrial DNA as a key factor and showing therapeutic potential of endothelial exosomes.

Structure of Orthoreovirus RNA Chaperone σNS, a Component of Viral Replication Factories

This paper determines the structure of the reovirus σNS RNA chaperone and shows how its oligomeric state and N-terminal arm regulate RNA binding and chaperone activity.

An amino acid-resolution interactome for motile cilia illuminates the structure and function of ciliopathy protein complexes

The paper defines an amino acid-resolution interactome of motile cilia using XL/MS in Tetrahymena thermophila, revealing structural and functional insights into ciliary machines and ciliopathy proteins.

Identification of small molecule inhibitors of G3BP-driven stress granule formation

This study identifies two small molecules that inhibit G3BP-driven stress granule formation and dissolve pre-existing granules, showing broad efficacy across cell types and stressors.

Non-specific recognition of histone modifications by H3K9bhb antibody

The paper shows that the H3K9bhb antibody also recognizes other modifications, including acetylations, which can affect interpretations of gene regulation data.

Label-free proteomic comparison reveals ciliary and non- ciliary phenotypes of IFT-A mutants

The paper applies DIFFRAC to study proteomic changes in Ift122 mutants of Tetrahymena thermophila, revealing both ciliary and non-ciliary effects of IFT-A loss.

The Human Mitochondrial Genome Encodes for an Interferon-Responsive Host Defense Peptide

This study identifies MOTS-c, a mitochondrial-encoded host defense peptide with antibacterial and immunomodulatory functions, showing that the immune system is encoded by both nuclear and mitochondrial genomes.

Humoral and T-cell-mediated responses to a pre-clinical Zika vaccine candidate that utilizes a unique insect-specific flavivirus platform

This study evaluates immune responses to an ISFV-based Zika vaccine, showing neutralizing antibodies and T-cells contribute to protection.

High-resolution assessment of multidimensional cellular mechanics using label-free refractive-index traction force microscopy

This paper introduces RI-TFM, a high-speed label-free technique for 3D cellular mechanics, and demonstrates its ability to measure traction forces and morphology in cells.

Age-dependent structural and morphological changes of the stem cell niche disrupt spatiotemporal regulation of stem cells and drive tissue disintegration

This study investigates age-related structural and morphological changes in the stem cell niche in C. elegans, showing how these changes disrupt Notch signaling and lead to tissue disintegration.

Identification of novel PGRP3 protein -protein interactions using yeast two hybrid system

This study identifies and validates two novel PGRP3-interacting proteins, MBNL3 and RBP5, using yeast two-hybrid screening and co-immunoprecipitation.

Baculoviral COVID-19 Delta DNA vaccine cross-protects against SARS-CoV2 variants in K18-ACE2 transgenic mice

This paper develops a baculoviral DNA vaccine targeting the SARS-CoV2 Delta variant, showing 100% survival against Delta and Omicron in mice, compared to 50% survival with the original vaccine.

PERIOD phosphorylation leads to feedback inhibition of CK1 activity to control circadian period

This study shows that PER phosphorylation inhibits CK1 activity, stabilizing PER and extending circadian period through feedback inhibition.

Endogenous circadian reporter cell lines as an efficient platform for studying circadian mechanisms

The paper develops Per1-luc and Per2-luc reporter cell lines to study circadian mechanisms and uncover differences between Per and Cry paralogs.