Author

J.-F. Trempe

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biochemistry· 8 Cell Biology· 2 Biophysics· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 10 Organism:human· 6 Research Focus:cell type annotation· 5 Research Focus:cell signaling & trafficking· 4 Assay:proteomics· 4 Research Focus:chromatin & epigenetic regulation· 2 Research Focus:drug response / drug discovery· 2 Organism:mouse· 2

Frequent Co-Authors

E. A. Fon· 5 shared N. Croteau· 4 shared S. Rasool· 4 shared S. Veyron· 4 shared A. A. Z. Khalifa· 2 shared C. Black· 2 shared K. H. Bui· 2 shared M. Eldeeb· 2 shared

Activity by Year

2019-2023 · peak 4/yr

Co-Author Collaboration Network

10 top collaborators · 25 mutual co-author links

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5 shared papersBiochemistry

E. A. Fon

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Author profile →

11 papers

Recent·Most cited

Identification and structural characterization of small-molecule inhibitors of PINK1

S. Rasool, T. Shomali, L. Truong, N. Croteau, S. Veyron, J.-F. Trempe

This study identifies and characterizes small-molecule inhibitors of PINK1, revealing their mechanism of action and potential for developing PD therapeutics.

bioRxiv · Oct 2, 2023Biochemistry

A genome-wide CRISPR/Cas9 screen identifies genes that regulate the cellular uptake of α-synuclein fibrils by modulating heparan sulfate proteoglycans

B. Vanderperre, A. Muraleedharan, M.-F. Dorion, F. Larroquette, E. Del Cid Pellitero, N. Rajakulendran, C. X.- Q. Chen, R. Lariviere, C. Michaud-Tardif, R. Chidiac, D. Lipuma, G. Macleod, R. Thomas, Z. Wang, W. E. Reintsch, , E. A. Fon

This study uses CRISPR/Cas9 screening to identify genes regulating α-synuclein fibril uptake via heparan sulfate proteoglycans, revealing SLC39A9 and C3orf58 as key modulators.

bioRxiv · Sep 29, 2023 · PreprintCell Biology

Eta-secretase-like processing of the amyloid precursor protein (APP) by RHBDL4

Y. C. M. Penalva, S. Paschkowsky, S. J. Recinto, A. Duchesne, C. Levet, F. Charron, M. Freeman, R. A. McKinney, J.-F. Trempe, L. M. Munter

This study identifies RHBDL4 as an enzyme that processes APP similarly to {eta}-secretase, generating A{eta}-like fragments in the ER without requiring other proteases.

bioRxiv · Sep 4, 2023 · PreprintBiochemistry

TOM20 Gates PINK1 Activity And Mediates Its Tethering Of The TOM And TIM23 Translocases Upon Mitochondrial Stress

M. Eldeeb, A. Bayne, A. Fallahi, T. Goiran, E. McDougall, A. Soumbasis, C. Zorca, J.-J. Tabah, R. Thomas, N. Karpilovsky, M. Mathur, T. Durcan, J.-F. Trempe, E. Fon

This study shows PINK1 forms a supercomplex with TOM and TIM23 during mitochondrial stress and is required for tethering TOM to TIM23, which is essential for mitophagy.

bioRxiv · Aug 7, 2023Biochemistry

Structure-based design and characterization of Parkin activating mutations

J.-F. Trempe, M. U. Stevens, N. Croteau, M. A. Eldeeb, Z. W. Zeng, R. Toth, T. M. Durcan, W. Springer, E. A. Fon, M. M. K. Muqit

The paper identifies three Parkin activating mutations that rescue mitophagy defects in cell-based studies, suggesting therapeutic potential for Parkinson's disease.

bioRxiv · Feb 22, 2022Biochemistry

Dissecting the interactions of PINK1 with the TOM complex in depolarized mitochondria

K. Maruszczak, M. Jung, S. Rasool, J.-F. Trempe, D. Rapaport

This study investigates how PINK1 interacts with the TOM complex in depolarized mitochondria, revealing the role of TOM70 and TOM40 in its association with the mitochondrial outer membrane.

bioRxiv · Jan 13, 2022 · PreprintBiochemistry

Selective localization of Mfn2 near PINK1 enable its preferential ubiquitination by Parkin on mitochondria

M. Vranas, Y. Lu, S. Rasool, N. Croteau, J. D. Krett, V. Sauve, K. Gehring, E. A. Fon, T. M. Durcan, J.-F. Trempe

The paper identifies Mfn2 as a preferred Parkin substrate due to its proximity to PINK1, which facilitates Parkin activation and ubiquitination on mitochondria.

bioRxiv · Aug 25, 2021Biochemistry

Structure of PINK1 reveals autophosphorylation dimer and provides insights into binding to the TOM complex

S. Rasool, S. Veyron, N. Soya, M. Eldeeb, G. Lukacs, E. A. Fon, J.-F. Trempe

The paper reports crystal structures of insect PINK1, revealing a dimeric autophosphorylation complex and insights into its binding to the TOM complex.

bioRxiv · Aug 5, 2021Biochemistry

Proteomic profiling of mitochondrial-derived vesicles in brain reveals enrichment of respiratory complex sub-assemblies and small TIM chaperones

R. F. Roberts, A. N. Bayne, T. Goiran, D. Levesque, F. M. Boisvert, J.-F. Trempe, E. A. Fon

The paper identifies proteins enriched in brain mitochondrial-derived vesicles (MDVs), revealing OXPHOS proteins and small TIM chaperones, and shows no significant difference in MDV proteomes between wild-type and PINK1-/- or Parkin-/- mice.

bioRxiv · Jul 6, 2020Biochemistry

The inner junction complex of the cilia is an interaction hub that involves tubulin post-translational modifications

A. A. Z. Khalifa, M. Ichikawa, D. Dai, C. Black, K. Peri, T. S. McAlear, S. Kubo, S. Veyron, S. K. Yang, J. Vargas, J.-F. Trempe, S. Bechstedt, K. H. Bui

The paper identifies new inner junction proteins in cilia and shows they form an interaction hub involving tubulin post-translational modifications.

bioRxiv · Oct 10, 2019Biophysics

Crystal structure of human PACRG in complex with MEIG1

N. Khan, D. Pelletier, S. Veyron, N. Croteau, M. Ichikawa, C. Black, A. A. Z. Khalifa, S. Chaaban, I. Kurinov, G. Brouhard, K. H. Bui, J.-F. Trempe

The paper reports the crystal structure of human PACRG with MEIG1 and models its role in microtubule junctions, revealing interactions with tubulin and evolutionary insights.

bioRxiv · Sep 27, 2019Cell Biology