K. Murakami

Structure and function of the Si3 insertion integrated into the trigger loop/helix of cyanobacterial RNA polymerase

This study presents the structure of Si3, a unique insertion in cyanobacterial RNA polymerase, and shows it does not affect catalytic activity despite large motion.

Chemical inhibition of stomatal differentiation by perturbation of the master-regulatory bHLH heterodimer via an ACT-Like domain

This study identifies Stomidazolone as a chemical inhibitor of the bHLH heterodimer MUTE-SCREAM, disrupting stomatal differentiation in plants.

Assessing climate change risks associated with precipitation at unfavorable times in winter wheat using an improved crop calendar model incorporating vernalization and winter survival

This study improves a crop phenology model for winter wheat by incorporating vernalization and winter survival, then assesses climate change risks under warming scenarios.

Homologous recombination deficiency in cell line libraries does not correlate with in vitro sensitivity to platinum agents or PARP inhibitors

This study finds that homologous recombination deficiency (HRD) in cancer cell lines is not associated with sensitivity to platinum agents or PARP inhibitors, contradicting clinical expectations.

Context-Dependent Modification of PFKFB3 in Hematopoietic Stem Cells Promotes Anaerobic Glycolysis and Ensures Stress Hematopoiesis

This study identifies PFKFB3 as a key regulator of glycolytic metabolism in HSCs under stress, showing its context-dependent activation promotes ATP production and hematopoiesis.

Inhibitory effect of Ephedra herba on human norovirus infection in human intestinal organoids

This study screens Kampo crude drugs for anti-HuNoV effects using human intestinal organoids, identifying Ephedra herba as a novel inhibitor targeting the post-entry step of infection.

Trade-off between grain yield and grain protein concentration is modulated by canopy photosynthesis in Japanese wheat cultivars

The paper examines the trade-off between grain yield and protein concentration in Japanese wheat cultivars, finding that stomatal behavior and canopy photosynthesis contribute to the inverse relationship.

Diagnosis and early prediction of lymphoma using high-throughput clonality analysis of bovine leukemia virus-infected cells

This study evaluates RAISING-CLOVA's ability to detect clonality in BLV-infected cells, showing high sensitivity and specificity in distinguishing EBL from non-EBL cattle and confirming its utility for early detection.

Evaluation of heat inactivation of human norovirus in freshwater clams using human intestinal enteroids

This study evaluates heat inactivation of human norovirus in freshwater clams using human intestinal enteroids, finding that 1 minute at 90°C inactivates the virus.

Dasabuvir inhibits human norovirus infection in human intestinal enteroids

This study identifies dasabuvir as an anti-HuNoV inhibitor using human intestinal enteroids, showing activity against HuNoV, SARS-CoV-2, and RVA.

Genomic differentiation within East Asian Helicobacter pylori

This study identifies genomic differentiation in Helicobacter pylori across East Asia, linking specific SNPs to cancer risk and geographic adaptation.

Structural visualization of de novo initiation of RNA polymerase II transcription

This study reports structures of RNA polymerase II initiation complexes showing TFIIH's role in promoter melting and transcription initiation.

Discovery of bactericides as an acute mitochondrial membrane damage inducer

The paper identifies Chlorhexidine and Alexidine as bactericides that induce acute mitochondrial membrane damage by altering inner mitochondrial membrane integrity and evoking a unique stress response.

Genome-wide association study of gastric cancer- and duodenal ulcer-derived Helicobacter pylori strains reveals discriminatory amino acid differences and novel oncoprotein candidates

This study uses GWAS to identify genetic variations in H. pylori strains associated with gastric cancer and duodenal ulcers, revealing amino acid differences and novel oncoprotein candidates.

Frequent PIK3CA mutations in eutopic endometrium of patients with ovarian clear cell carcinoma

This study identifies frequent PIK3CA mutations in eutopic endometrium of ovarian clear cell carcinoma patients, showing high mutation rates and intratumor heterogeneity.

Atomic structure of human sapovirus capsid by single particle cryo-electron microscopy

The paper reports the atomic structure of human sapovirus capsid using cryo-EM, revealing domain interactions and hypervariable regions important for immune response and receptor binding.

Structure of TFIIH/Rad4-Rad23-Rad33 in damaged DNA opening in Nucleotide Excision Repair

The paper presents a cryo-EM structure of an NER initiation complex involving Rad4-Rad23-Rad33 and TFIIH bound to a DNA lesion, revealing DNA unwinding and translocation mechanisms.

Genome-Scale CRISPR Screening reveals novel factors regulating Wnt-dependent regeneration of mouse gastric organoids

The paper uses CRISPR screening to identify novel factors regulating Wnt-dependent regeneration of mouse gastric organoids, finding that Alk, Bclaf3, and Prkra suppress Wnt-driven self-renewal.

Cryo-EM Structures of Respiratory bc1-cbb3 type CIII2CIV Super-complex and Electronic Communication Between the Complexes

This paper reports the first cryo-EM structure of a bacterial respiratory bc1-cbb3 super-complex and native CIII2 conformers, revealing structural and electronic communication features between complexes.

Dynamic rotation of the protruding domain enhances the infectivity of norovirus

This study shows that the protruding domain of norovirus capsids dynamically rotates between rising and resting conformations in response to pH and metal ions, enhancing infection efficiency.

Adaptive thermogenesis in mice requires adipocyte light-sensing via Opsin 3

This study shows that blue light sensed by OPN3 in adipocytes regulates thermogenesis and lipolysis in mice.

Structural basis of HEAT-kleisin interactions in the human condensin I subcomplex

This study reports the crystal structure of the human condensin I subcomplex hCAP-G and hCAP-H, revealing their interaction and DNA binding capabilities essential for mitotic chromosome assembly.