D. Qian

Temperature-induced changes in protein interactions control RNA recruitment to G3BP1 condensates

The paper investigates how temperature affects G3BP1-RNA phase separation, revealing that while the phase boundary remains stable, stoichiometry and energetics shift, driven by entropic processes.

Linking modulation of bio-molecular phase behaviour with collective interactions

This paper introduces a dominance analysis framework to determine the molecular mechanism of condensate modulation, demonstrating its application to G3BP1/RNA condensates disrupted by suramin.

The crystal and cryo-EM structures of PLCγ2 reveal dynamic inter-domain recognitions in autoinhibition

The paper determines crystal and cryo-EM structures of PLCγ2 in autoinhibited states and bound to FGFR1 kinase domain, revealing dynamic inter-domain interactions in autoinhibition.

Dominance Analysis: A formalism to uncover dominant energetic contributions to biomolecular condensate formation in multicomponent systems

This paper introduces a formalism to identify dominant energetic contributions to biomolecular condensate formation in multicomponent systems by analyzing phase separation data.

Quantifying collective interactions in biomolecular phase separation

This study quantifies collective interactions in biomolecular phase separation by analyzing dilute phase concentrations and free energy, revealing how salts and small molecules modulate condensate formation.

3D organoid modeling identified that targeting IGF1R signaling may overcome drug resistance in breast cancer

The paper develops a 3D organoid model to identify drug combinations that overcome breast cancer resistance, finding that IGF1R inhibition enhances treatment efficacy.

Modulating Nucleic Acid Phase Transitions as a Mechanism of Action for Cell-Penetrating Antimicrobial Peptides

This study identifies that antimicrobial peptides (AMPs) induce nucleic acid phase separation, which may be a novel mechanism for their antibacterial activity.

Adsorption of RNA to interfaces of biomolecular condensates enables wetting transitions

This study shows that pyrimidine-rich RNAs act as adsorbents enabling wetting transitions in condensates formed by purine-rich RNAs, revealing orientation differences between scaffolds and adsorbents.

Multi-dimensional protein solubility optimization with an ultra-high-throughput microfluidic platform

This paper introduces a high-throughput microfluidic platform for protein solubility screening, enabling rapid and efficient optimization of formulation additives and mutations.

Analytical solution to the Flory-Huggins model

The paper derives an analytical solution for the Flory-Huggins model, showing its ability to extend phase space validity and reveal exponential scaling laws for protein phase separation.

Tie-lines reveal interactions driving heteromolecular condensate formation

The paper develops a method to determine tie-line gradients in ternary LLPS systems using dilute phase concentration measurements, revealing interaction types and compositions in biomolecular condensates.

Multiphase condensates from a kinetically arrested phase transition

This paper shows that multiphase biomolecular condensates form due to kinetic trapping during phase transitions, not thermodynamic stability, and explains how this leads to internal sub-compartments.

LMO2 is critical for early metastatic events in breast cancer

The paper identifies LMO2-expressing tumor cells as metastasis-initiating cells and shows LMO2-STAT3 signaling promotes breast cancer metastasis.

Supraphysiological testosterone induces ferroptosis and activates NF-kappaB mediated immune pathways in prostate cancer through nucleophagy