M. Kirchner

Intestinal interstitial fluid isolation provides novel insight into the human host-microbiome interface

The paper develops methods to collect intestinal interstitial fluid from different segments and shows segment-specific differences in microbiota-derived metabolites, cytokines, and proteins compared to plasma and serum.

Human ADA2 deficiency is characterized by the absence of an intracellular hypoglycosylated form of adenosine deaminase 2

The paper identifies an intracellular hypoglycosylated form of ADA2 absent in DADA2 patients, linking its absence to reduced deaminase activity and variant pathogenicity.

Prdm16 mutation determines sex-specific cardiac metabolism and identifies two novel cardiac metabolic regulators

This study investigates monoallelic Prdm16 mutation in mice, revealing sex-specific cardiac metabolic dysregulation and identifying Pyroxd2 and Pbxip1 as novel metabolic regulators.

Amplicon structure creates collateral therapeutic vulnerability in cancer

The paper identifies DDX1 as a passenger gene co-amplified with MYCN in cancer, showing that its aberrant expression creates a therapeutic vulnerability through mTORC1 dependency.

Single organoid RNA-sequencing reveals high organoid-to-organoid variability

This study uses RNA-seq to show that organoids vary significantly between individuals within a culture, but not between batches, suggesting microenvironmental effects drive this variability.

Heterogeneity in Proline Hydroxylation of Fibrillar Collagens Observed by Mass Spectrometry

The paper uses mass spectrometry to analyze proline hydroxylation in collagens, finding heterogeneity in hydroxylation patterns across different collagen types and sources.

Protective immune trajectories in early viral containment of non-pneumonic SARS-CoV-2 infection

This study identifies an early interferon-stimulated gene (ISG) upregulation as a protective immune signature in non-pneumonic SARS-CoV-2 infection, preventing progression to pneumonia.

Exploiting a PAX3-FOXO1-induced synthetic lethal ATR dependency for rhabdomyosarcoma therapy

This study identifies ATR as a synthetic lethal target in PAX3-FOXO1-driven rhabdomyosarcoma, showing ATR inhibition enhances PARP inhibitor efficacy and is tolerated in patients.

Soluble tubulin is locally enriched at mitotic centrosomes in C. elegans

The paper measures tubulin concentrations at mitotic centrosomes in C. elegans embryos, showing localized enrichment of soluble β-tubulin.

Protein Interaction Screen on Peptide Matrix (PRISMA) reveals interaction footprints and the PTM-dependent interactome of intrinsically disordered C/EBPβ

The paper uses PRISMA to map C/EBPβ's PTM-dependent interactome, revealing interaction footprints and functional links to chromatin and RNA processing.

Picky: a simple method designer for targeted proteomics

Picky is a tool that automatically selects informative peptides for targeted proteomics methods, addressing the challenge of optimizing SRM and PRM assays.

Mutations In Disordered Regions Cause Disease By Creating Endocytosis Motifs

The paper identifies mutations in disordered regions that create dileucine motifs, leading to clathrin-dependent trafficking and disease.