T. Ou

In vivo affinity maturation of the HIV-1 Env-binding domain of CD4

This study demonstrates that non-antibody protein biologics can undergo affinity maturation in vivo, leading to improved binding affinity and neutralization potency against HIV-1.

In vivo affinity maturation of murine B cells reprogrammed to express human antibodies

This study demonstrates that CRISPR-edited murine B cells expressing human antibodies can undergo affinity maturation, generating potent neutralizing antibodies against HIV-1 and SARS-CoV-2.

Heavy-chain CDR3-engineered B cells facilitate in vivo evaluation of HIV-1 vaccine candidates

The paper evaluates HIV-1 vaccine candidates using HCDR3-engineered B cells, finding that SOSIP-TM mRNA-lipid nanoparticles induce stronger anti-apex responses than multimerized SOSIP proteins.

Efficient reprogramming of the heavy-chain CDR3 regions of a human antibody repertoire

The paper reprograms human B cells to express HCDR3 regions of HIV-1 bNAbs, enabling diverse and physiologic repertoires that efficiently bind HIV-1 antigens.

Infection and chronic disease activate a brain-muscle signaling axis that regulates muscle performance

This study identifies a brain-muscle signaling axis activated by infections and chronic diseases that reduces muscle performance through cytokine-dependent mechanisms.

Hydroxychloroquine-mediated inhibition of SARS-CoV-2 entry is attenuated by TMPRSS2

This paper shows hydroxychloroquine inhibits SARS-CoV-2 entry via cathepsin L but not TMPRSS2, and that combining it with a TMPRSS2 inhibitor enhances antiviral effects.

Co-translational protein targeting facilitates centrosomal recruitment of PCNT during centrosome maturation

The paper shows PCNT is co-translationally targeted to centrosomes during mitosis by cytoplasmic dynein, with ASPM also showing similar targeting to spindle poles.