B. Huang

Molecular Architecture of Monkeypox Mature Virus

This study uses cryo-ET and STA to reveal the architecture of mature MPXV particles and identifies irregular virions with distorted core walls.

De Novo Design of Integrin α5β1 Modulating Proteins for Regenerative Medicine

The paper designs de novo proteins called NeoNectins that specifically bind integrin α5β1, enhancing cell attachment and tissue integration in biomaterials.

Multispectral live-cell imaging with uncompromised spatiotemporal resolution

The paper introduces a multispectral imaging approach for live-cell imaging, enabling accurate unmixing of low signal-to-noise ratio data at video rates.

INSIHGT: Accessible multimodal systems biology with quantitative molecular phenotyping in 3D

The paper introduces INSIHGT, a 3D spatial biology method for multi-modal molecular phenotyping, and demonstrates its application in mapping cellular structures and identifying novel cell types in human and mouse tissues.

Adipocyte microRNA-802 promotes adipose tissue inflammation and insulin resistance by modulating macrophages in obesity

This study shows that miR-802 in adipose tissue promotes inflammation and insulin resistance by modulating macrophage recruitment and polarization in obesity.

Design of High Affinity Binders to Convex Protein Target Sites

This paper designs high-affinity proteins that bind to convex protein sites using geometrically matching concave scaffolds, achieving low nanomolar to picomolar affinities.

Atomically accurate de novo design of antibodies with RFdiffusion

This paper designs antibodies de novo with atomic precision using RFdiffusion and yeast display, validated by cryo-EM and biophysical methods.

Bottlebrush polyethylene glycol nanocarriers translocate across human airway epithelium via molecular architecture enhanced endocytosis

This paper demonstrates that bottlebrush PEG nanocarriers can translocate across human airway epithelium via enhanced endocytosis due to their unique molecular architecture.

A Germinal Center Checkpoint of AIRE in B Cells Limits Antibody Diversification

This study identifies AIRE as a checkpoint in germinal center B cells that limits antibody diversification by inhibiting AID function, leading to autoimmunity and impaired immune responses.

Mantis: high-throughput 4D imaging and analysis of the molecular and physical architecture of cells

The paper introduces Mantis, a high-throughput 4D microscope, and shrimPy, software for analyzing 4D data, to study cellular dynamics and responses to perturbations like viral infection.

HybriSeq: Probe-based Device-free Single-cell RNA Profiling

HybriSeq enables sensitive single-cell RNA profiling by combining in situ hybridization, split-pool barcoding, and sequencing to detect transcripts without end bias.

De novo designed Hsp70 activator dissolves intracellular condensates

The paper designs Hsp70 activators that dissolve intracellular condensates by modulating Hsp70 ATPase activity and promoting protein refolding.

Biodecomposing Spirulina platensis by a de novo designed Bacillus subtilis-based method to develop a medium for the high cell-density cultivation of Escherichia coli in batch mode

This paper develops a method to biodecompose Spirulina platensis using Bacillus subtilis to create a medium for high cell-density cultivation of Escherichia coli, achieving higher growth rates and optical density than traditional LB broth.

Alopecosa nagpag acts on cardiac ventricular myocytes to kill prey

This study identifies the venom of Alopecosa nagpag and shows it affects cardiac ion currents in neonatal rat ventricular myocytes, potentially causing prey death by altering cardiac rhythm.

Long COVID manifests with T cell dysregulation, inflammation, and an uncoordinated adaptive immune response to SARS-CoV-2

This study identifies T cell dysregulation, inflammation, and mis-coordinated adaptive immune responses in Long COVID patients compared to non-LC individuals.

Structure of human NaV1.6 channel reveals Na+ selectivity and pore blockade by 4,9-anhydro-tetrodotoxin

The paper presents cryo-EM structures of human NaV1.6 channels with and without 4,9-anhydro-tetrodotoxin, revealing Na+ selectivity and inhibition mechanisms.

Deep Learning Assisted Smartphone-based Quantitative Microscopy for Label-free Hematological Analysis

This paper introduces a smartphone-based autofluorescence microscopy system enhanced with deep learning for label-free hematological analysis, enabling rapid and accurate blood cell classification.

Label-free and non-destructive pathology of human lung adenocarcinomas with ultraviolet single-plane illumination microscopy

This paper introduces MUSI, a rapid, label-free imaging method for lung adenocarcinomas, showing it can differentiate subtypes with resolution comparable to FFPE histology.

Differences in gene expression in field populations of Wolbachia-infected Aedes aegypti mosquitoes with varying release histories in northern Australia

This study examines gene expression changes in Wolbachia-infected Aedes aegypti mosquitoes with different release histories, finding persistent immune-related gene upregulation despite reduced gene expression changes over time.

Comprehensive structural analysis reveals broad-spectrum neutralizing antibodies against Omicron

This study identifies antibodies effective against Omicron by analyzing epitope changes and binding patterns, showing their ability to neutralize the virus through a conserved mechanism.

Improving de novo Protein Binder Design with Deep Learning

This paper improves de novo protein binder design by using deep learning models to assess design success rates and computational efficiency.

A Chitin-Binding Protein Ultra-highly expressed in the Outer Fold of Mantle is Related to Shell Colour in Pacific Oyster Crassostrea gigas

This study identifies a chitin-binding protein, CgCBP, highly expressed in the outer fold of the mantle of Pacific oyster Crassostrea gigas, which is linked to shell pigmentation through its role in periostracum structure.

Randomized gates eliminate bias in sort-seq assays

The paper introduces randomized sorting in sort-seq assays to eliminate systematic bias, validated through simulation and experiments, and extends the method for variance estimation and multi-bin sorting.

Oncogenic protein condensates suppress growth factor perception and modulate drug tolerance

This study shows that oncogenic protein condensates suppress RTK signaling and modulate drug tolerance in ALK-driven cancers by sequestering adapter proteins and enabling paracrine survival signals.

Observing noncovalent interactions in experimental electron density for macromolecular systems: A novel perspective for protein-ligand interaction research

The paper introduces a database of non-covalent interactions in protein-ligand complexes using experimental electron density data and demonstrates its application in studying amide-pi interactions.

Potential inhibitors for blocking the interaction of the coronavirus SARS-CoV-2 spike protein and its host cell receptor ACE2

This paper identifies TS-984 as a potent inhibitor of the SARS-CoV-2 spike protein-ACE2 interaction through a high-throughput screening platform and in vitro validation.

De Novo Pyrimidine Synthesis is a Targetable Vulnerability in IDH Mutant Glioma

This study identifies de novo pyrimidine synthesis as a therapeutic target in IDH mutant gliomas, showing that DHODH inhibitors are effective in preclinical models.

A focal adhesion kinase-YAP signaling axis drives drug tolerant persister cells and residual disease in lung cancer

This study identifies a FAK-YAP signaling axis that drives drug-tolerant persister cells and residual disease in lung cancer, showing that inhibiting this axis improves tumor response to targeted therapies.

Short-term instantaneous prophylaxis and efficient treatment against SARS-CoV-2 in hACE2 mice conferred by an intranasal nanobody (Nb22)

This paper demonstrates that intranasal nanobody Nb22 provides short-term instantaneous prophylaxis and effective treatment against SARS-CoV-2 in hACE2 mice, with ultrahigh potency and long-lasting retention in the respiratory system.

Single-shot rAAV5-based Vaccine Provides Long-term Protective Immunity against SARS-CoV-2 and Its Variants

This study develops a single-dose rAAV5-based vaccine that provides long-term immunity against SARS-CoV-2 and its variants with high neutralizing antibody titers and safety.