flow cytometry

This study shows that intestinal helminth infection reduces T cell responses to an mRNA SARS-CoV-2 vaccine and weakens protection against Omicron but not the ancestral strain.

The paper identifies three immunologically distinct groups in lupus nephritis patients based on blood immunophenotyping, correlating with histopathology, treatment response, and renal outcomes.

This study shows that Parp14 deficiency in mice worsens colitis by impairing epithelial barrier integrity and altering inflammation-related gene expression.

This study shows that injecting MOC1 cells orthotopically in the buccal mucosa of mice leads to tumor growth, lymph node enlargement, and changes in immune cell composition over time.

The paper demonstrates the presence of strain-transcending antibodies against rosetting Plasmodium falciparum strains in humans using flow cytometry and antibody elution experiments.

The paper identifies a distinct cluster of GDF15high macrophages with anti-inflammatory properties using single-cell RNA sequencing and functional assays.

The paper develops and validates the tviblindi algorithm to identify branching developmental trajectories in human B cell development using mass cytometry data.

The paper develops a mass cytometry method to track T cell receptor repertoires and differentiation states during infection and immunotherapy, showing how antigen recognition influences T cell responses.

The paper identifies novel therapeutic targets for AIEC infection by stratifying macrophages based on bacterial load and showing that targeting these pathways reduces AIEC replication.

This study shows that combining radiotherapy with CpG, a TLR9 agonist, enhances tumor response in a mouse sarcoma model by boosting CD8 T cell activity.

This paper develops a deep learning pipeline for automated AML diagnosis and molecular characterization using flow cytometry data, achieving high accuracy in classification and variant prediction.

The paper compares methods for simultaneously quantifying yeast and bacterial cells in mixed suspensions, finding that flow cytometry, Coulter counter, and Spread-plate methods are statistically similar for yeast, while microscopy and Drop-plate are similar for bacteria.

The paper shows cancer causes systemic CD8+ T cell homeostatic dysregulation, leading to uncontrolled differentiation and poor immune checkpoint inhibitor response.

This study identifies a novel subclonal population of proliferating CLL cells characterized by high CXCR4 and CD5 expression, which correlates with disease progression and clinical relapse.

This paper shows that AXL inhibits cholesterol mobilization in dendritic cells, which is necessary for their maturation and immunogenic response to cancer.

The paper identifies CD8+FOXP3+ Tregs enriched in human tissues, expressing intracellular CD25 and tissue residency markers, and demonstrates their therapeutic potential and association with disease states.

This study examines T cell exhaustion markers in SLE, finding that CXCR5+ CD8 T cells express high IFN-gamma and Tim3, suggesting a role in autoimmune progression and tolerance.

The paper evaluates the depletion of T cell subsets in nonhuman primates using monoclonal antibodies, finding substantial depletion in blood and tissue with residual cells often remaining in tissue.

The paper develops an improved workflow for quantifying orthohantavirus infection using automated imaging and flow cytometry, with an open-source software tool.

This study shows that Fat1 is not required for normal murine haematopoiesis, as knockout mice show no significant differences in blood cell populations or function.

The paper shows that CD4+ T cells reduce inflammation and disease severity during Mtb reinfection by reprogramming CD8+ T cells and innate immune cells.

The paper identifies CD4+CD57+PD1+ T cells in lung transplant recipients that predict allograft dysfunction and are associated with fibrosis in both human and mouse models.

This study shows antibiotic treatment after primary Mtb infection reduces lung inflammation but does not eliminate protection against reinfection in macaques.

The paper identifies novel stages in IgG memory B cell and antibody-secreting cell differentiation by analyzing antigen-specific B cells from different pathogens using high-dimensional flow cytometry.

This study shows that chemotherapy, specifically CarboTaxol, synergizes with cancer vaccines and anti-PD-1 to improve tumor control and survival by expanding stem-like TCF1+CD8+ T cells.

This study investigates immune dynamics during Neisseria musculi colonization in mice, identifying key genes and pathways for immune homeostasis and offering insights for vaccine development.

The paper develops a 50-color flow cytometry panel for comprehensive immune cell phenotyping, including T cells and dendritic cells, and provides a 45-color version for broader use.

This study uses Bac-CyTOF to analyze immune cell interactions with Klebsiella pneumoniae in mice, revealing changes in immune cell populations and the role of T6SS and STING in infection outcomes.

This paper uses multi-parameter flow cytometry and machine learning to characterize platelet sub-populations and their responses to agonists, achieving high accuracy in distinguishing platelet age and activation states.