Lei Wang

A synchronization modulation electric field reduces early ischemic kidney injury in pigs and suppresses a stress and inflammatory program without detectable leukocyte accumulation

Modified 3rd Generation Synchronization Modulation Electric Field (3m-SMEF) Prevents Ischemia-Reperfusion-Induced Acute Kidney Injury via Targeting Na⁺/K⁺-ATPase

The paper demonstrates that modified 3rd-generation SMEF (3m-SMEF) protects against ischemia-reperfusion-induced AKI by preserving Na/K-ATPase function and tubular integrity in vitro and in vivo.

Cross-species multi-omics integration reveals lung alterations in acute kidney injury across mouse, pig, and human airway organoid models

This paper integrates multi-omics data across species to show AKI-induced lung alterations, identifying conserved pathways and human-validated targets for distant organ failure.

Niche-dependent epistasis within the cancer genome shapes metastatic fitness

The paper maps gene-by-gene-by-environment epistasis in cancer metastasis using CRISPR perturbations in mice, showing niche-dependent fitness and transcriptome convergence.

Systematic CRISPRi perturbation of 1,408 autism risk genes maps multilevel transcriptional convergence in human cortical neurons

This study uses CRISPRi Perturb-seq to analyze 1,408 autism risk genes in human cortical neurons, identifying 215 genes that cause global transcriptomic dysregulation and uncovering shared transcriptional pathways.

Attenuated Salmonella-Mediated Delivery of GSDMD Potentiates PD-1 Blockade Therapy against Melanoma

This study combines attenuated Salmonella-delivered GSDMD with anti-PD-1 therapy to enhance anti-melanoma immune responses in mice.

Architectural trade-offs between environmental stability and genomic redundancy reveal divergent pneumoviral entry strategies

The paper compares RSV and hMPV entry strategies, showing RSV uses structural organization for thermotolerance while hMPV uses genomic redundancy for stability.

Selective GRAB sensors reveal distinct endocannabinoid dynamics in vivo

The paper develops selective fluorescent sensors to detect endocannabinoid dynamics in vivo and shows that Δ9-THC induces sustained 2-AG signaling in the nucleus accumbens.

PROTAC-Mediated Targeting of IKKβ and NR4A1 for AML Therapy

This paper identifies IKKβ and NR4A1 as drivers of AML and develops a PROTAC to degrade them, showing potent cytotoxicity and reduced leukemia progression in mice.

A hierarchical activation threshold shapes RSV F antibody repertoires from toddler vulnerability to adult protection

This study identifies a hierarchical activation threshold in RSV F antibody repertoires, explaining pediatric vulnerability and adult protection through distinct antibody subclasses and epitope targeting.

Gut Microbiota-derived Acetate Safeguards the Colonic Epithelial Acetyl-CoA Reserve to Avert Colonic Senescence

The paper shows that acetate from gut microbiota maintains acetyl-CoA levels in colonic epithelial cells, preventing age-independent senescence and inflammation.

Olfactomedin 4 marks luminal progenitor cells that give rise to a secretory cell lineage in the mouse cervix

The paper identifies Olfm4 as a marker for luminal progenitor cells in the mouse cervix that give rise to secretory goblet cells, revealing a species similarity with humans.

Experimental SARS-CoV-2 infection using horseshoe bats

This study experimentally infects horseshoe bats with SARS-CoV-2, showing low-level persistent viral replication without disease, and identifies host mechanisms that enable viral coexistence.

Damaging the conical morphology of HIV-1 capsid by targeting the FG-binding pocket and disfavoring pentameric subunits needed for core closure

This study investigates how ZW-1261, an FGBP-targeting inhibitor, disrupts HIV-1 capsid morphology by affecting pentameric subunits and core closure.

pertTF: context-aware AI modeling for genome-scale and cross-system perturbation prediction

The paper introduces pertTF, a transformer-based model for predicting genetic perturbation responses, showing improved performance in expression prediction and cell identity shifts across cellular contexts.

A genetically encoded fluorescent sensor for monitoring spatiotemporal prostaglandin E2 dynamics in vivo

This paper develops a genetically encoded fluorescent sensor, GRABPGE2-1.0, to monitor spatiotemporal PGE2 dynamics in vivo with high resolution.

Computational nanobody design using graph neural networks and Metropolis Monte Carlo sampling

This paper develops a computational method using graph neural networks and Metropolis Monte Carlo sampling to design high-affinity nanobodies, achieving a Pearson correlation of 0.62 on benchmark.

Induction of meningioma stem signature via hydrogel reprogramming and application of meningioma stem cell marker CXCR4 to pathological diagnosis and treatment.

The paper identifies CXCR4 as a meningioma stem cell marker induced by hydrogel reprogramming and demonstrates its potential for diagnostic and therapeutic applications.

Harmonized Protocol for Subfield Segmentation in the Hippocampal Body on High-Resolution in vivo MRI from the Hippocampal Subfields Group (HSG)

The paper develops a harmonized segmentation protocol for hippocampal subfields in MRI, improving cross-study comparisons and understanding of subfield structure and function.

Structural insights into the recognition and catalysis of tRNA by human NSUN2

This study determines the structure of human NSUN2 in complex with tRNA substrates, revealing its mechanism of substrate recognition and catalytic specificity, and identifies a small-molecule inhibitor.

Proteolysis targeting chimera extracellular vesicles for therapeutic development treating triple negative breast cancer

This paper demonstrates that microfluidic droplet-based electroporation (microDES) enhances PROTAC drug delivery via extracellular vesicles, improving therapeutic efficacy and safety in a TNBC mouse model.

Process optimization for enhanced enzymatic nylon deconstruction

This study optimizes reaction conditions for three nylon-degrading enzymes, achieving high product titers for PA6 and PA66 without enzyme engineering or substrate pretreatment, and demonstrates scalability for complex PA-based materials.

KiF1a-regulated neuronal infrastructures in sensory and prefrontal cortices essential for fear-relevant anxiety

The paper investigates how psychological stress induces fear and anxiety by altering neuronal infrastructures in sensory and prefrontal cortices, with KiF1a-mediated transport playing a critical role.

Considerations for the use of contrast agents with diffuse in vivo flow cytometry to detect circulating cancer cell populations

This paper evaluates contrast agents for in vivo fluorescence detection of circulating tumor cells, finding that while they show high uptake, they also label non-cancer cells, reducing specificity.

Enhancer regulatory networks globally connect non-coding breast cancer loci to cancer genes

The paper uses single-cell CRISPRi screens to show variant-associated enhancers regulate cancer genes in breast cancer, revealing network motifs and subtype-specific functions.

Upcycling Polyethylene into Poly(3-hydroxybutyrate) via a Chemo-Enzymatic-Microbial Cascade

This paper develops a chemo-enzymatic-microbial system to convert polyethylene into poly(3-hydroxybutyrate), achieving 71.19% weight loss and 16.75 mg/L PHB concentration.

Caveolin-1 regulates context-dependent signaling and survival in Ewing Sarcoma.

This study identifies Caveolin-1 as a context-dependent regulator of survival signaling in Ewing Sarcoma, revealing a reversible CD99High state with enhanced survival.

Chromosome-level Genome Assembly and Annotation of Petunia hybrida

This paper presents a chromosome-level genome assembly for Petunia hybrida using hybrid sequencing technologies, revealing syntenic relationships and providing a resource for functional genomics.

Fast Targeted Metabolomics for Analyzing Metabolic Diversity of Bacterial Indole Derivatives in ME/CFS Gut Microbiome

This paper develops a fast targeted metabolomics method to analyze indole derivatives in ME/CFS gut microbiome samples, revealing metabolic differences that may serve as biomarkers.

Longevity or Well-being? A Dual-Dimension Structure of Neuroticism

The paper proposes that neuroticism variability can be structured into two dimensions, one linked to longevity and the other to well-being, with distinct neural and genetic underpinnings.