M. Fitzgerald

Genome-wide CRISPRa screens nominate modulators of CAR T cell survival within distinct tumor cytokine milieus

This paper develops a CRISPRa-based method to identify genes enhancing CAR T cell survival in different tumor environments.

Multiplexed screen identifies a Pseudomonas aeruginosa-specific small molecule targeting the outer membrane protein OprH and its interaction with LPS

The paper identifies a Pseudomonas aeruginosa-specific small molecule, BRD1401, that targets OprH and disrupts its interaction with LPS, increasing membrane fluidity.

Extracellular vesicles carrying HIV-1 Nef induce trained immunity in myeloid cells

The paper shows that HIV-1 Nef-containing extracellular vesicles induce trained immunity in myeloid cells through epigenetic changes and lipid raft upregulation, leading to persistent inflammation.

Chemical-genetic interaction mapping links carbon metabolism and cell wall structure to tuberculosis drug efficacy

This study maps chemical-genetic interactions in Mycobacterium tuberculosis to identify how carbon metabolism and cell wall structure affect drug efficacy, revealing environment-dependent and -independent interactions.

Ultra-deep sequencing differentiates patterns of skin clonal mutations associated with sun-exposure status and skin cancer burden

This study uses ultra-deep sequencing to analyze clonal mutations in sun-exposed and non-sun-exposed skin, finding higher UV-signature mutations and mutation burden in sun-exposed samples.

A porcine ex vivo lung perfusion model to investigate bacterial pathogenesis

This paper develops a porcine ex vivo lung perfusion model to study Klebsiella pneumoniae-induced pneumonia, showing it recapitulates lung injury and immune responses similar to mouse models.

Large-scale chemical-genetics yields new Mycobacterium tuberculosis inhibitor classes

This study uses a novel chemical-genetics screening strategy to identify new inhibitors of Mycobacterium tuberculosis, revealing novel compound classes targeting previously unexplored bacterial targets.