M. Schmitz

optiPRM: A targeted immunopeptidomics LC-MS workflow with ultra-high sensitivity for the detection of mutation-derived tumor neoepitopes from limited input material

The paper introduces optiPRM, a targeted LC-MS workflow that enhances sensitivity for detecting mutation-derived neoepitopes from limited tumor samples.

Structural basis of Cdk7 activation by dual T-loop phosphorylation

This study reports the crystal structure of fully activated Cdk7/Cyclin H/Mat1 complex with dual T-loop phosphorylations, revealing their distinct roles in kinase activity and substrate recognition.

The gut microbiome-linked long chain fatty acid stearate suppresses colorectal cancer

This study shows that the ketogenic diet reduces colorectal tumors in mice by altering the gut microbiome to increase stearic acid, which suppresses cancer cell growth and immune responses.

Actin associates with actively elongating genes and binds directly to the Cdk9 subunit of P-TEFb

This study identifies a direct interaction between actin and Cdk9 in P-TEFb, showing actin binds to actively elongating genes and inhibits polymerization during transcription.

CD38 promotes hematopoietic stem cell dormancy via c-Fos

This study identifies CD38 as a marker for dormant hematopoietic stem cells and shows it promotes dormancy through the cADPR/Ca2+/c-Fos/p57Kip2 pathway.

The genomic basis of hybrid male sterility in Ficedula flycatchers

The paper identifies candidate genes for hybrid male sterility in birds using genomic and transcriptomic data, revealing chromosomal and chromatin-related issues in hybrid spermatogenesis.

An Alpha-helical Lid Guides the Target DNA toward Catalysis in CRISPR-Cas12a

This study reveals how the alpha-helical lid in CRISPR-Cas12a guides the DNA target strand toward the RuvC catalytic core through molecular dynamics simulations and experiments.

Structural basis for RNA-mediated assembly of type V CRISPR-associated transposons

This study presents a cryo-EM structure of a CRISPR-associated transposon complex, revealing RNA-mediated assembly mechanisms and the role of ribosomal protein S15 in transposition.

cGAS/STING-DEPENDENT SENSING OF ENDOGENOUS RNA

The paper shows that loss of SAMHD1 leads to chronic DNA damage and cGAS/STING-dependent type I interferon production in mice, linking it to autoimmunity and cancer.

Appetite regulating genes in zebrafish gut; a gene expression study

This study investigates appetite-regulating gene expression in zebrafish gut under different feeding conditions, identifying leptin-dependent differential expression of anorexigenic genes.

Molecular mechanism of target site selection and remodeling by type V CRISPR-associated transposons

This study elucidates the molecular mechanism of RNA-directed DNA transposition by type V-K CRISPR-associated transposons using cryo-EM structures and biochemical assays.

The active repertoire of Escherichia coli peptidoglycan amidases varies with physiochemical environment

This study identifies pH-dependent activity of E. coli amidases AmiB and AmiC, mediated by EnvC, NlpD, and YgeR, revealing non-redundant roles in cell separation.

Immune Landscapes Predict Chemotherapy Resistance And Immunotherapy Response In Acute Myeloid Leukemia

This study identifies immune subtypes in AML and shows that IFN-gamma profiles predict chemotherapy resistance and immunotherapy response.

Spheroid culture of mesenchymal stromal cells results in morpho-rheological properties appropriate for improved microcirculation

This study shows that culturing mesenchymal stromal cells in 3D spheroids improves their morpho-rheological properties, enhancing their ability to circulate through microvascular networks.