P. Joshi

Cell-surface glycans are quantitative reporters of Golgi dysfunction in single cells

The paper quantifies how surface glycans reflect Golgi dysfunction in single cells using fluorescent lectin probes and a mathematical model.

Regenerative human liver organoids (HLOs) in a pillar/perfusion plate for hepatotoxicity assays

This paper develops a bioprinted liver organoid system for high-throughput hepatotoxicity assays, showing improved maturity, reproducibility, and regenerative capacity after drug exposure.

Dynamic culture of cerebral organoids using a pillar/perfusion plate for the assessment of developmental neurotoxicity

This study evaluates developmental neurotoxicity using dynamic cerebral organoid cultures in a pillar/perfusion plate, showing methylmercury induces changes in morphology and gene expression under dynamic conditions but not static ones.

Cell-state plasticity drives heterogeneity in Group 3/4 medulloblastoma

This study identifies cell-state plasticity as a driver of heterogeneity in Group 3/4 medulloblastomas, showing that transcription factor perturbation can redirect tumor cells along different differentiation trajectories.

Medulloblastoma oncogene aberrations are not involved in tumor initiation, but essential for disease progression and therapy resistance

The paper uses single-cell technologies to show that large-scale chromosomal aberrations drive medulloblastoma initiation, while single-gene oncogenic events like MYC contribute to progression and therapy resistance.

Orientation Single Molecule Localization Microscopy (oSMLM) for Decoding Orientation of Single Molecule

This paper introduces oSMLM to decode the orientation of single molecules from PSF shape and direction, achieving 20 nm localization precision and 5-degree orientation precision.

A mechanistically novel peptide agonist of the IL-7 receptor that addresses limitations of IL-7 cytokine therapy

This paper introduces a novel peptide agonist of the IL-7 receptor that mimics IL-7's biological activity but has improved therapeutic properties and reduced immunogenicity.

Host inducible-HSP70A1A is an irresistible drug target to combat SARS-CoV-2 infection and pathogenesis

This study shows that inhibiting host Hsp70 reduces SARS-CoV-2 infection more effectively than Remdesivir by blocking viral entry and autophagy.

Uniform cerebral organoid culture on a pillar plate by simple and reproducible spheroid transfer from an ultralow attachment well plate

This paper introduces a method to generate reproducible cerebral organoids from iPSCs using a pillar plate platform, improving throughput and reducing variability compared to traditional methods.

Identification of merozoite secreted repertoire and immuno-pharmacological inhibition of a novel host-parasite interaction to block malarial infection

This study identifies the secretory repertoire of Plasmodium merozoites and discovers a peptide that disrupts a key host-parasite interaction, blocking malarial infection.

A Pillar and Perfusion Plate Platform for Robust Human Organoid Culture and Analysis

This paper introduces a microarray 3D bioprinting platform with pillar and perfusion plates for high-throughput human organoid culture and analysis.

A pillar/perfusion plate enhances cell growth, reproducibility, throughput, and user friendliness in dynamic 3D cell culture

The paper introduces a pillar/perfusion plate for dynamic 3D cell culture, improving growth, reproducibility, throughput, and user friendliness compared to static methods.

Cardiac sympathetic denervation prevents sudden cardiac arrest and improves cardiac function by enhancing mitochondrial-antioxidant capacity

This study investigates how cardiac sympathetic denervation prevents sudden cardiac arrest and improves cardiac function by enhancing mitochondrial-antioxidant capacity in a pressure-overload heart failure model.

Ryanodine receptor 2 inhibition reduces dispersion of cardiac repolarization, improves contractile function and prevents sudden arrhythmic death in failing hearts

Single-Molecule Super-Resolution Microscopy Reveals Formation of NS2B3 Protein Clusters on Mitochondrial Network Leading to its Fragmentation during the Onset of Dengue (Denv-2) Viral Infection

This paper uses single-molecule super-resolution microscopy to study NS2B3 protein cluster formation on mitochondria during dengue virus infection, revealing a correlation with mitochondrial fragmentation.

Comparative analysis of drug-salt-polymer interactions by experiment and molecular simulation improves biopharmaceutical performance

The paper combines experiments and molecular simulations to identify optimal drug-salt-polymer combinations for improving biopharmaceutical performance of poorly water-soluble drugs.

Simultaneous Multisheet Multichannel Imaging Cytometry (SMMIC) System Powered by Adaptive Vertically-Aligned Multi-Sheet Array (VAMSA) PSF

The paper introduces a high-throughput imaging cytometry system (SMMIC) that enables volumetric interrogation of live specimens with high resolution and flow rate.

A two-step in vivo CRISPR screen unveils pervasive RNA binding protein dependencies for leukemic stem cells and identifies ELAVL1 as a therapeutic target

This study identifies RNA binding proteins essential for leukemic stem cell survival using an in vivo CRISPR screen and highlights ELAVL1 as a therapeutic target in AML.

Scanning Single Molecule Localization Microscopy (scanSMLM) for super-resolution optical volume imaging

This paper introduces scanSMLM, a scanning SMLM system for rapid 3D volume imaging, demonstrating its ability to visualize HA clustering in 3D cells and quantify biophysical parameters.

Detection of Fortunate Molecules Induce PArticle Resolution Shift (PAR-shift) towards Single-molecule Limit in SMLM: A Technique for Resolving Molecular Clusters in Cellular System

The paper introduces leSMLM, a technique that detects fortunate molecules to improve spatial resolution in SMLM, achieving a PAR-shift of 6 nm and 11 nm with longer exposure times.

Cellular development and evolution of the mammalian cerebellum

This study uses single-nucleus RNA-sequencing to compare cerebellar development across human, mouse, and opossum, revealing conserved and species-specific gene expression patterns.

Mapping pediatric brain tumors to their origins in the developing cerebellum

The paper maps the cellular origins of three common pediatric brain tumors using a single-nucleus atlas and transcriptome data, identifying lineage-specific origins and potential therapeutic targets.

Vitamin A and vitamin E metabolites comodulate amyloid-β aggregation

This study identifies retinoic acid and tocopherol as vitamin metabolites that modulate amyloid-β aggregation in vitro and in a C. elegans model, with combined effects that may cancel each other.

Lightsheet Optical Tweezer (LOT) for Optical Manipulation of Microscopic Particles and Live Cells

The paper develops a light sheet optical tweezer (LOT) to trap and pattern microscopic particles and live cells, showing increased stiffness and single-degree-of-freedom movement.

Multi-omics profiling of U2AF1 mutants dissects pathogenic mechanisms affecting RNA granules in myeloid malignancies

This study uses multi-omics profiling to analyze U2AF1 mutations in myeloid malignancies, revealing altered RNA binding, splicing, and stress granule formation.

High Throughput Volume Flow Cytometry (parallel-iLIFE) Resolves Mitochondrial Network On the Go

This paper introduces parallel-iLIFE, a high-throughput volume flow cytometry method that enables real-time analysis of mitochondrial networks in HeLa cells with sub-cellular resolution.

In silico analysis of long non-coding RNAs in medulloblastoma and its subgroups

The paper performs an in silico analysis of long non-coding RNAs in medulloblastoma, identifying subgroup-specific lncRNAs and developing diagnostic and prognostic models.

Transcriptome stability profiling identifies novel and functional MIRNA targets in melanoma cells

The paper uses RNA stability profiling to identify novel miR-211 targets in melanoma cells and validates their functional role in cell invasion and colony formation.

Quantifying the mechanics of locomotion of the schistosome pathogen with respect to changes in its physical environment

This study quantifies the biomechanics of schistosome locomotion in different physical environments and identifies key motility parameters that could inform drug development.

Integrated RNA and metabolite profiling of urine liquid biopsies for prostate cancer biomarker discovery

The paper identifies urine-enriched RNAs and metabolites in prostate cancer patients and normal individuals, revealing altered glutamate metabolism and TCA cycle in cancer cells.