J. Frank

Time resolution in cryo-EM using a novel PDMS-based microfluidic chip assembly and its application to the study of HflX-mediated ribosome recycling

This paper introduces a microfluidic chip for time-resolved cryo-EM to study HflX-mediated ribosome recycling, capturing reaction intermediates at high resolution.

Structural insight into translation initiation of the ????cl leaderless mRNA

This study uses cryo-EM to reveal structural insights into the translation initiation of leaderless mRNA by examining the uS2-deficient ribosome and its interaction with the lambda cI lmRNA.

Cryo-EM studies of the four E. coli paralogs establish ABCF proteins as master plumbers of the peptidyl-transferase center of the ribosome

This study presents cryo-EM structures of four E. coli ABCF paralogs bound to the ribosome, revealing their role in modulating tRNA geometry and catalytic function at the peptidyl-transferase center.

Expanding Interdisciplinarity: A bibliometric study of medical education using the MEJ-24

The paper uses bibliometric analysis of 31,283 articles to assess the interdisciplinary nature of medical education research, finding limited field diversity but increasing category diversity over time.

Mechanism of dual pharmacological correction and potentiation of human CFTR

This paper uses cryo-EM to reveal how VX445 corrects CFTR folding and gating defects by altering allosteric communication pathways.

Microneurography as a Minimally Invasive Method to Assess Target Engagement During Neuromodulation

This study compares microneurography electrodes to cuff and LIFE electrodes in measuring neural signals during neuromodulation, finding microneurography can detect activation thresholds and sensory activity.

The Voices of Medical Education Science: Describing the Published Landscape

This paper analyzes authorship patterns in medical education literature from 2000-2020, finding gender and geographic disparities in publication.

Geometric machine learning informed by ground truth: Recovery of conformational continuum from single-particle cryo-EM data of biomolecules

This paper introduces an unsupervised geometric machine learning approach to recover conformational continua from single-particle cryo-EM data using simulated ground-truth datasets, showing improved results over previous methods.

Divergent epigenetic profiles from two differentially impacted wild populationsof estuarine cordgrass (Sporobolus alterniflorus)

This study examines how two populations of estuarine cordgrass show opposite DNA methylation patterns under heat stress, suggesting epigenetic mechanisms may enable rapid adaptation to urban environmental challenges.

A glycan gate controls opening of the SARS-CoV-2 spike protein

This study identifies a glycan gate and key residues that facilitate SARS-CoV-2 spike protein RBD opening through simulations and experimental validation.

An activity-based labelling method for the detection of ammonia and methane-oxidizing bacteria.

This paper introduces an activity-based labelling method to detect and identify ammonia- and methane-oxidizing bacteria in complex environments.

Enrichment and physiological characterization of a novel comammox Nitrospira indicates ammonium inhibition of complete nitrification

This study enriches and characterizes a novel comammox Nitrospira species, revealing its high ammonia affinity and ammonium inhibition, suggesting niche differentiation based on ammonium tolerance.

Metagenomic profiling of ammonia- and methane-oxidizing microorganisms in a Dutch drinking water treatment plant

This study profiles ammonia- and methane-oxidizing microorganisms in a Dutch drinking water treatment plant using metagenomic sequencing, identifying key microbial taxa and their distribution across different filtration stages.

POLARIS: path of least action analysis on energy landscapes

The paper introduces POLARIS, a tool for automatically finding the path of least action on 2D energy landscapes and provides visualization and analysis features.

Simulation of cryo-EM ensembles from atomic models of molecules exhibiting continuous conformations

This paper simulates cryo-EM ensembles from atomic models of molecules with continuous conformations to test machine learning methods for free energy landscape reconstruction.

The Israeli Acute Paralysis Virus IRES captures host ribosomes by mimicking a ribosomal state with hybrid tRNAs.

The paper characterizes the IAPV IRES structure using cryo-EM to show how it captures host ribosomes and redirects them to viral mRNA, providing insights for RNA-interference strategies against CCD.

Metagenomic analysis reveals large potential for carbon, nitrogen and sulfur cycling in coastal methanic sediments of the Bothnian Sea

The paper analyzes metagenomic data from coastal sediments in the Bothnian Sea to reveal metabolic capabilities related to carbon, nitrogen, and sulfur cycling.

Functional Pathways of Biomolecules Retrieved from Single-particle Snapshots

The paper introduces a method to analyze conformational changes in biomolecules using single-particle cryo-EM data, revealing functional pathways in RyR1 that differ from discrete structure analyses.

The structural basis for release factor activation during translation termination revealed by time-resolved cryogenic electron microscopy

This paper uses time-resolved cryo-EM to directly observe the transition of bacterial release factors RF1 and RF2 from a compact to extended conformation during translation termination.

Identification of changing ribosome protein compositions using cryo-EM and mass spectrometry

The paper uses cryo-EM and mass spectrometry to show that yeast ribosomes change their protein composition in response to growth medium shifts.

Conformational Dynamics and Energy Landscapes of Ligand Binding in RyR1

The paper uses cryo-EM data to analyze conformational dynamics and energy landscapes of ligand binding in RyR1, revealing multiple binding routes and structural changes.