J. Rae

Proteostatic regulation of caveolins avoids premature oligomerisation and preserves ER homeostasis

This study investigates proteostatic regulation of caveolin-1 and caveolin-3, showing how their trafficking and oligomerisation are controlled to prevent ER stress and disease.

Macrophages only sense infectious SARS-CoV-2 when they express sufficient ACE2 to permit viral entry, where rapid cytokine responses then limit viral replication.

The paper shows that ACE2-expressing macrophages allow SARS-CoV-2 entry and replication, triggering anti-viral responses, while ACE2-negative macrophages do not support viral entry or cytokine release.

YhcB coordinates peptidoglycan and LPS biogenesis with phospholipid synthesis during Escherichia coli cell growth.

This study identifies YhcB as a coordinator of peptidoglycan, LPS, and phospholipid synthesis in E. coli, revealing its role in envelope stability and cell size control.

Cavin1 intrinsically disordered domains are essential for fuzzy electrostatic interactions and caveola formation

This study shows that Cavin1's intrinsically disordered domains are essential for caveola formation and trafficking through electrostatic interactions and phase separation.

Muscle-specific Cavin4 interacts with Bin1 to promote T-tubule formation and stability in developing skeletal muscle

This study identifies Cavin4's role in T-tubule formation and stability in skeletal muscle by showing its interaction with Bin1 and effects on caveolae remodeling.

Nanoscape, a data-driven 3D real-time interactive virtual cell environment

Nanoscape provides a 3D interactive virtual cell environment using scientific data and artistic design for real-time exploration of cellular structures.

APEX-Gold: A genetically-encoded particulate marker for robust 3D electron microscopy

APEX-Gold converts APEX reaction products into silver/gold particles for improved EM detection of tagged proteins.

Cargo-specific recruitment in clathrin and dynamin-independent endocytosis

The paper identifies swiprosin-1 as a cargo-specific adaptor for the CG-pathway, showing its role in integrin internalization and breast cancer progression.

An inverted Caveolin-1 topology defines a novel exosome secreted from prostate cancer cells

The paper identifies an inverted Cav1 topology in exosomes secreted by prostate cancer cells, revealing a non-canonical autophagy pathway involved in disease progression.

An Endocytic Capture Model for Skeletal Muscle T-tubule Formation

This study develops a four-dimensional model of T-tubule formation in zebrafish using live imaging and electron microscopy, proposing an endocytic capture mechanism involving dynamic endocytic tubules, myofibrils, and membrane delivery from recycling endosomes and Golgi.

Dissecting the nanoscale lipid profile of caveolae

This study defines the caveolar lipid profile using quantitative lipid mapping and simulations, showing how CAV1 and cavin1 sort distinct lipids and create a unique lipid environment.

Piezo1 Induces Local Curvature in a Mammalian Membrane and Forms Specific Protein-Lipid Interactions

This study shows Piezo1 induces membrane curvature and forms specific lipid interactions, influencing both membrane morphology and lipid composition.

Small GTPases and BAR domain proteins regulate branched actin to make clathrin and dynamin independent endocytic vesicles

This study identifies the molecular machinery regulating clathrin and dynamin-independent endocytic vesicles, showing the sequential recruitment of ARF1/GBF1 and CDC42, and the roles of BAR domain proteins and actin polymerization.