D. Smith

Transcriptional regulation in the absence of inositol trisphosphate receptor calcium signaling

This study examines how the loss of IP3R calcium signaling affects transcriptional regulation and identifies three key adaptations in IP3R TKO cells.

Mapping the Cellular Biogeography of Human Bone Marrow Niches Using Single-Cell Transcriptomics and Proteomic Imaging

This study maps human bone marrow niches using single-cell transcriptomics and proteomic imaging, identifying distinct cellular environments and their roles in hematopoiesis and AML.

Lipid A double bond position determination using ozone and laser-induced dissociation

This study determines the double bond position in Lipid A using ozone treatment on MALDI plates combined with tandem MS.

Fitting a square beam in a square camera: novel condensor apertures for low-dose transmission electron microscopy

This paper introduces square and rectangular condenser apertures for TEM to improve sample area efficiency in low-dose applications, demonstrating compatibility with cryo-TEM.

Impact of angiotensin-receptor blockers on intrarenal renin-angiotensin system activity in hypertension: A PK/PD modelling study

This study uses PK/PD modeling to show ARBs like Losartan more effectively inhibit intrarenal RAS by blocking Ang II uptake, reducing downstream signaling and normalizing blood pressure in rats.

Structural Elucidation of Intact Rough-Type Lipopolysaccharides using Field Asymmetric Ion Mobility Spectrometry and Kendrick Mass Defect Plots

This paper uses FAIMS and Kendrick mass defect plots to characterize the structural diversity of R-type lipopolysaccharides from E. coli J5.

The long and short of it: Benchmarking viromics using Illumina, Nanopore and PacBio sequencing technologies

This paper benchmarks viromics using Illumina, Nanopore, and PacBio sequencing technologies to assess their impact on viral community reconstruction and error rates.

A Single Laccase Acts as a Key Component of Environmental Sensing in a Broad Host Range Fungal Pathogen

This study identifies Sslac2, a laccase in Sclerotinia sclerotiorum, as critical for environmental sensing and virulence, showing its role in pathogenesis and host interaction.

A single-administration therapeutic interfering particle reduces SARS-CoV-2 viral shedding and pathogenesis in hamsters

A single dose of therapeutic interfering particles reduces SARS-CoV-2 shedding and transmission in hamsters.

A unified peptide array platform for antibody epitope binning, mapping, specificity and predictive off-target binding

This paper introduces a unified peptide array platform for high-resolution antibody epitope binning, mapping, and off-target binding prediction, demonstrating its application with anti-HER2 monoclonal antibodies.

Persistent Immune and Clotting Dysfunction Detected in Saliva and Blood Plasma after COVID-19

The paper identifies persistent immune and clotting dysfunction in saliva and plasma of COVID-19 patients 4-6 weeks post-symptom resolution, highlighting saliva as a viable alternative to plasma for monitoring immune and inflammatory sequelae.

The intrarenal renin-angiotensin system in hypertension: Insights from mathematical modelling

This study uses computational modeling to show that AT1R-mediated uptake, not increased local Ang II production, drives intrarenal Ang II accumulation during Ang II infusion, contributing to hypertension.

A Useful Guide to Lectin Binding: Machine-Learning Directed Annotation of 57 Unique Lectin Specificities

The paper uses machine learning and manual annotation to define the glycan-binding specificities of 57 lectins based on microarray data.

Peptide Scanning of SARS-CoV and SARS-CoV-2 Spike Protein Subunit 1 Reveals Potential Additional Receptor Binding Sites

This paper identifies additional receptor binding sites on SARS-CoV and SARS-CoV-2 spike protein subunit 1 through peptide scanning and validates their ACE2 binding affinity.

The development of ovine gastric and intestinal organoids for studying ruminant host-pathogen interactions

This paper develops ovine gastric and intestinal organoids to study host-pathogen interactions in ruminants, showing they maintain tissue-like transcriptomes and can be infected by pathogens.

AgMESH, a peritrophic matrix-associated protein embedded in Anopheles gambiaemelanotic capsules modulates malaria parasite infection

The paper identifies AgMESH, a mosquito protein associated with melanin-based immunity, and shows its role in peritrophic matrix integrity and malaria parasite infection.

Toxoplasma TgATG9 is critical for autophagy and long-term persistence in tissue cysts

This study shows that TgATG9 is essential for autophagy and long-term survival of Toxoplasma gondii bradyzoites in tissue cysts.

High-affinity P2Y2 and low-affinity P2X7 receptor interaction modulates ATP-mediated calcium signaling in murine osteoblasts

This study investigates how P2Y2 and P2X7 receptors interact to modulate ATP-induced calcium signaling in osteoblasts, revealing biphasic responses and dynamic interplay between receptor subtypes.

Benzofuran sulfonates and small self-lipid antigens activate type II NKT cells via CD1d

This study identifies benzofuran sulfonates and small self-lipid antigens as activators of type II NKT cells via CD1d, revealing non-lipidic small molecules that trigger CD1d-restricted NKT cell responses.

Perivascular Space Semi-Automatic Segmentation (PVSSAS): A Tool for Segmenting, Viewing and Editing Perivascular Spaces

The paper introduces PVSSAS, a tool for semi-automated segmentation of perivascular spaces, showing high agreement with manual methods.

Immunological memory to SARS-CoV-2 assessed for up to eight months after infection

The paper assesses SARS-CoV-2 immune memory components over eight months post-infection, finding varying kinetics in antibodies, B cells, and T cells.

Toxoplasma Cathepsin Protease B and Aspartyl Protease 1 play recessive roles in endolysosomal protein digestion during infection

This study shows that CPL is the dominant protease in Toxoplasma gondii's VAC, while CPB and ASP1 have dispensable roles in protein digestion.

Evolutionary origins of epidemic potential among human RNA viruses

The paper investigates the evolutionary origins of epidemic potential in human RNA viruses, finding that most transmissible lineages evolved from non-human viruses.

Alcohol increases aggression in flies

The paper identifies two pathways by which alcohol increases aggression in male flies: one through enhancing an olfactory pheromone and the other through systemic alcohol effects.

Activation loop dynamics are controlled by conformation-selective inhibitors of ERK2

This paper shows how different ERK2 inhibitors shift conformational states by exploiting activation loop dynamics, affecting dephosphorylation rates.

Data from an electronic health informatics pipeline to describe clearance dynamics of Hepatitis B surface antigen (HBsAg) and e-Antigen (HBeAg) in chronic HBV infection

The paper describes an electronic health informatics pipeline that collects longitudinal clinical data on HBsAg and HBeAg clearance in chronic HBV infection, revealing demographic and treatment-related factors associated with clearance.

Drug Repurposing: The Anthelmintics Niclosamide and Nitazoxanide are Potent TMEM16A Antagonists that Fully Bronchodilate Airways

This paper identifies niclosamide and nitazoxanide as potent TMEM16A antagonists that fully bronchodilate airways, offering new treatment options for severe asthma and COPD.

Motile curved bacteria are Pareto-optimal

The paper uses in silico experiments to show that curved bacterial shapes are Pareto-optimal, balancing swimming efficiency, chemotaxis, and cell construction cost.

One of the gang: social group dynamics in a juvenile passerine bird

The paper studies social group dynamics in juvenile hihi birds, finding that groups are composed mainly of juveniles who stay at sites longer than adults and associate primarily with other juveniles.

Gene cluster conservation identifies melanin and perylenequinone biosynthesis pathways in multiple plant pathogenic fungi

The paper identifies perylenequinone and melanin biosynthesis pathways in plant pathogenic fungi by analyzing gene cluster conservation and confirming roles through mutagenesis and metabolite analysis.