M. Fernandez

Engineering oncogenic hotspot mutations on SF3B1 via CRISPR-directed PRECIS mutagenesis

This paper develops PRECIS, a method combining prime editing and a synthetic intron reporter to efficiently generate SF3B1 mutant cell lines that recapitulate cancer-related splicing and CNV events.

The Origin And Speciation Of Orchids

This study reconstructs the phylogeny of orchids using genomic data and infers their geographic origin and speciation patterns, finding their origin in Laurasia and recent speciation in Central America.

Ubiquitous purine sensor modulates diverse signal transduction pathways in bacteria

The paper identifies a purine-binding motif in bacterial receptors and demonstrates its role in modulating second messenger signaling.

Biodiversity monitoring in Europe: user and policy needs

The paper identifies gaps in biodiversity monitoring in Europe and recommends establishing a coordinated network to improve data harmonization and policy response to biodiversity loss.

Tailoring evidence into action: using a codesign approach for biodiversity information in the Tropical Andes

This study develops a codesign approach to improve biodiversity information flow in the Tropical Andes by engaging stakeholders to create tailored, FAIR-compliant indicators.

AFM-based force spectroscopy unravels the stepwise-formation of a DNA transposition complex driving multi-drug resistance dissemination

The paper uses atomic force microscopy to study the stepwise formation of a DNA transposition complex involved in antibiotic resistance dissemination.

Aldosterone mediates potassium-induced kidney fibrosis through tubular and mesenchymal mineralocorticoid receptor activation

This study shows high dietary potassium worsens kidney fibrosis in mice by activating MR in tubular and mesenchymal cells, mediated by aldosterone.

Wolbachia interacts with the microbiome to shape fitness-associated traits during seasonal adaptation in Drosophila melanogaster

This study shows Wolbachia limits microbial diversity and interacts with the microbiome to influence fitness traits in Drosophila during seasonal adaptation.

B-cell epitope mapping of TprC and TprD variants of Treponema pallidum subspecies

This study identifies B-cell epitopes in TprC and TprD variants of Treponema pallidum using pepscan mapping and antisera, showing cross-reactivity and surface-exposed loop targeting.

Transcriptional and immunological analysis of the putative outer membrane protein and vaccine candidate TprL of Treponema pallidum

This paper investigates the transcriptional regulation of the TprL gene in Treponema pallidum, showing that phase variation through homopolymeric G sequences affects its expression and immune response.

Host evolutionary history and ecological context modulate the adaptive potential of the microbiome

This study shows that host genotype, microbiome, and environment interact to influence host fitness, with mismatches causing fitness costs in a high-sugar diet.

BATF3-dependent induction of IL-27 by B cells bridges the innate and adaptive stages of the antibody response

B cells integrate innate TLR and adaptive CD40 signals to induce BATF3 transcription factor for production of IL-27, which together with INFg optimizes antibody responses.

Antagonistic pathogen-mediated selection favours the maintenance of innate immune gene polymorphism in a widespread wild ungulate

The study investigates how pathogen-mediated selection maintains innate immune gene polymorphism in roe deer, finding antagonistic effects of Toxoplasma and Chlamydia on Tlr2 polymorphism.

Altered frequency of CD24highCD38high transitional B cells in patients with cardiac involvement of chronic Chagas disease.

The paper examines B cell subpopulations in chronic Chagas disease patients with and without cardiac involvement, finding increased transitional CD24highCD38high B cells and IL-10-producing B cells in those with cardiac disease.

CD4+ T cells from chronic Chagas disease patients with different degrees of cardiac compromise exhibit distinct expression patterns of inhibitory receptors TIGIT, Tim-3 and Lag-3.

Finding the signal in the noise of Citizen Science Observations

The paper evaluates how citizen science projects can improve biodiversity monitoring by collecting contextual information to reduce noise and enhance signal accuracy, using eBird as a case study.