I. Dikic

Intrinsically disordered region amplifies membrane remodeling to augment selective ER-phagy

This study investigates the role of intrinsically disordered regions (IDRs) in ER-phagy by modeling and simulating their membrane interactions and remodeling effects.

Development of covalent probes to capture Legionella pneumophila effector enzymes

The paper develops covalent probes to target Legionella pneumophila effector enzymes, demonstrating their effectiveness in capturing DupA through in vitro testing and structural analysis.

Engineering CRBN for rapid identification of next generation binders

This paper introduces a new CRBN construct for efficient binder discovery, showing it outperforms existing constructs in binding and production, and applies it to identify potent CRBN binders from a large compound library.

Serine ubiquitination of p62 regulates Nrf2 dependent redox homeostasis

This study shows that serine ubiquitination of p62 by Legionella pneumophila SidE effectors inhibits Nrf2 signaling, altering redox homeostasis and host metabolism during infection.

Targeted protein degradation via intramolecular bivalent glues

This paper introduces intramolecular bivalent glues (IBGs) as a new method for targeted protein degradation, showing they enhance ubiquitination by bridging protein domains and improving degradation efficiency.

Stabilisation of β-Catenin-WNT signalling by USP10 in APC-truncated colorectal cancer drives cancer stemness and enables super-competitor signalling

This study identifies USP10 as a deubiquitylase that stabilizes β-Catenin in APC-truncated colorectal cancer, enhancing WNT signaling and cancer stemness.

TBC1D15 potentiates lysosomal regeneration from damaged membranes

The paper identifies TBC1D15 as a key regulator of lysosomal regeneration from damaged membranes through a pathway involving LIMP2, ATG8, and ALR components.

An mTOR-independent Macroautophagy Activator Ameliorates Tauopathy and Prionopathy Neurodegeneration Phenotypes

The paper identifies CCT020312, an mTOR-independent autophagy activator, which reduces tauopathy and prionopathy neurodegeneration phenotypes by enhancing autophagy flux and clearing protein aggregates.

USP28 enables oncogenic transformation of respiratory cells and its inhibition potentiates molecular therapy targeting mutant EGFR, BRAF and PI3K.

This study shows USP28 promotes lung cancer by stabilizing oncogenes and its inhibition enhances therapy for EGFR, BRAF, and PI3K mutant tumors.

Simeprevir suppresses SARS-CoV-2 replication and synergizes with remdesivir

The paper identifies simeprevir as a potent inhibitor of SARS-CoV-2 replication that synergizes with remdesivir in vitro.

FAM134B-RHD Protein Clustering Drives Spontaneous Budding of Asymmetric Membranes

The paper uses molecular dynamics simulations to show that FAM134B-RHD clusters drive membrane budding in the ER, influenced by curvature and bilayer asymmetry.

Characterization of protease activity of Nsp3 from SARS-CoV-2 and its in vitro inhibition by nanobodies

This study characterizes Nsp3 protease activity from SARS-CoV-2 and identifies nanobodies that inhibit PLpro with nanomolar affinity.

Proteomics of autophagy deficient macrophages reveals enhanced antimicrobial immunity via the oxidative stress response

This study shows that loss of Atg16l1 in macrophages enhances antimicrobial immunity against Shigella flexneri via the oxidative stress response.

Inhibition of USP28 overcomes Cisplatin-Resistance of Squamous Tumors by Suppression of the Fanconi Anemia Pathway.

This study identifies USP28 inhibition as a strategy to overcome cisplatin resistance in squamous cell carcinomas by suppressing the Fanconi Anemia DNA repair pathway.

Combinatorial CRISPR screening reveals functional buffering in autophagy

This study uses combinatorial CRISPR screening to identify gene interactions in autophagy, revealing functional buffering and redundant gene circuits.

Serine-ubiquitination regulates Golgi morphology and the secretory pathway upon Legionella infection

This study shows that Legionella's SdeA effector causes Golgi disruption via serine ubiquitination of GRASP55 and GRASP65, affecting the secretory pathway and cytokine release.

The Kinase Chemogenomic Set (KCGS): An open science resource for kinase vulnerability identification

The paper introduces the Kinase Chemogenomic Set (KCGS), a curated collection of 187 selective kinase inhibitors covering 215 human kinases, for use in cell-based screens.

Wss1 promotes replication stress tolerance by degrading histones.

This study identifies Wss1 as a protease that degrades histones to promote replication stress tolerance in yeast.

TBK1-mediated phosphorylation of LC3C and GABARAP-L2 controls autophagosome shedding by ATG4 protease

The paper shows TBK1 phosphorylates LC3C and GABARAP-L2, preventing their removal by ATG4 and ensuring proper autophagosome formation.

Molecular recognition of M1-linked ubiquitin chains by native and phosphorylated UBAN domains

This study investigates how phosphorylation affects UBAN domain binding to M1-linked ubiquitin chains, revealing structural and functional differences among related proteins.

Structural and Functional analysis of the GABARAP Interaction Motif (GIM).

The paper identifies a GABARAP-specific interaction motif (GIM) and demonstrates its selectivity over LC3 using biophysical and structural analyses.