N. W. Snyder

This study shows that chemotherapy-induced senescence-associated secretome promotes metastasis in ovarian cancer by reprogramming metabolism and increasing cell detachment.

This study shows ATR enhances mTORC1 activity through cholesterol synthesis via LSS, independent of CHK1 and TSC, and this effect is amplified in p16-deficient cells.

This study shows that lactoylglutathione (LGSH) promotes inflammatory signaling in macrophages by increasing histone lactoylation and chromatin accessibility.

This study identifies ANO6-mediated trogocytosis as a mechanism by which CAFs transfer lipids to PDAC cells, promoting tumor growth and immune suppression.

This study identifies de novo purine metabolism as a vulnerability in cancers with low p16 expression, showing sensitivity to anti-folate drugs like methotrexate.

This study shows ACOT2 regulates acyl-CoA levels in glycolytic muscle, promoting β-oxidation when lipid supply is low but causing lipid accumulation and poor glucose homeostasis when lipid supply is high.

The paper compares colorimetric, fluorometric, and LC-MS-based assays for measuring acetyl-CoA, finding that LC-MS/MS and LC-HRMS provide reliable results with internal standards, while colorimetric ELISA fails even with standards.

This study shows that dynamic protein deacetylation in Acly-/- MEFs contributes carbons to acetyl-CoA but only transiently supplies about 9% of cellular acetyl-CoA.

This study identifies the carnitine shuttle and CrAT as a pathway for mitochondrial-to-cytosolic acetyl-CoA transfer, independent of ACLY, in cancer cells.

The paper shows that reducing coenzyme A biosynthesis increases cancer cells' sensitivity to puromycin, a selection antibiotic used in research.

This study shows that exogenous CoA enhances TLR-driven proinflammatory and antimicrobial responses in macrophages by promoting oxidative metabolism and histone acetylation.

This study shows that ketones like beta-hydroxybutyrate reduce premature aging features in Cockayne syndrome models by enhancing transcriptional readthrough of DNA secondary structures through histone acetylation.

This study shows that thymidine rescues ATR kinase inhibitor-induced deoxyuridine contamination, cell death, and Type 1 interferon expression in CD8+ T cells.

This study shows persistent DNA damage alters lipid metabolism and histone acetylation via MYS-1/Tip60, leading to immune-like responses and age-related decline.

This study identifies OGT's role in regulating GBM acetate metabolism through CDK5-dependent ACSS2 phosphorylation, showing that targeting this pathway inhibits tumor growth.

This study investigates the electrophilicity of saturated oxo-fatty acids and finds that mono-saturation retains electrophilicity, while double saturation abolishes it, implicating PTGR1 in their metabolism.

This study identifies SIRT5 as essential for melanoma cell survival and tumor formation, showing it regulates chromatin dynamics and gene expression through histone modifications.

The paper introduces SILEC-SF, a method for sub-cellular metabolomic analysis, and finds distinct nuclear acyl-CoA profiles with notable propionyl-CoA enrichment and a new metabolic pathway for histone propionylation.

The paper confirms the in vivo synthesis of a six-carbon metabolite from propionate in human heart and mouse tissues through non-oxidative pathways.

This study shows that ATM inhibition promotes cancer cell survival by increasing macropinocytosis, which can be suppressed by BCAA supplementation.

This paper quantifies lactoyl-CoA in mammalian cells and tissues using LC-HRMS and provides a biosynthetic method for isotopically labeled lactoyl-CoA.

This study shows that acetate metabolism in cumulus cells decreases with maternal age and correlates with oocyte maturity.

The paper corrects for artifactual post-harvest metabolism in subcellular isotope tracing to reveal compartment-specific metabolic kinetics.

This study shows that p16 suppression increases nucleotide synthesis through mTORC1 activation, promoting tumorigenesis by bypassing senescence.

This study identifies IDH1 as a therapeutic target in HGSC by showing its role in promoting metabolic adaptation and epigenetic repression, leading to senescence when inhibited.

This paper develops a method to simultaneously quantify and trace dNTP metabolites using isotope dilution and high-resolution mass spectrometry, showing improved accuracy and metabolic pathway insights.

The paper identifies an artefactual pyruvate formation from lactate during LC-MS analysis, highlighting the need for improved resolution or quantification in metabolomics studies.

This paper introduces bioorthogonal pro-metabolites to profile short chain fatty acylation in cells, identifying novel protein targets and characterizing their metabolic and enzymatic effects.